scholarly journals Effects of specific inhibition of sterol biosynthesis on the uptake and utilization of low density lipoprotein cholesterol by HepG2 cells.

1991 ◽  
Vol 32 (10) ◽  
pp. 1657-1665
Author(s):  
SR Panini ◽  
GT Everson ◽  
TA Spencer
Keyword(s):  
Hepg2 Cells ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Sterol Biosynthesis ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Specific Inhibition ◽  
Low Density Lipoprotein Cholesterol

scholarly journals Antioxidant potential and low-density lipoprotein cholesterol (LDL-c) uptake of the black seed and honey mixture on human hepatocellular carcinoma (HepG2) cells

Food Research ◽  
2021 ◽  
Vol 5 (6) ◽  
pp. 141-149
Author(s):  
N.S. Mohd Isa ◽  
J.S. Ng ◽  
F. Tufail Ahmad ◽  
M.N.I. Kassim ◽  
Norhayati H. ◽  
...  
Keyword(s):  
Hepg2 Cells ◽  
Antioxidant Activities ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Lipid Lowering ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Black Seed ◽  
Significant Difference

High lipid levels especially low-density-lipoprotein cholesterol (LDL-c) are associated with increased risk of cardiovascular (CVD) and coronary heart disease (CHD). Both black seed (Nigella sativa L.) and honey are well-known in the hypolipidemic potential and have CVD protective effects. In the present study, LDL-c uptake of the black seed and honey mixture was tested on HepG2 cells. Antioxidant activities of black seed and honey mixtures were determined through the 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) assay. The anticancer potential of black seed and honey mixtures in HepG2 cells was performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay. Black seed possessed the highest antioxidant activities with EC50 6.54 mg/mL as compared to honey with EC50 value 9.56 mg/mL while the black seed and honey mixture have EC50 between black seed and honey. From the results obtained, no synergistic effect was observed in the mixtures as the EC50 values were within the range of black seed and honey. Furthermore, no significant difference (p>0.05) among ratios (1:1, 2:1 and 1:2). However, the decrease in cell proliferation was the highest in black seed and honey mixture at 1:1 ratio (p<0.05) than individually treated black seed and honey. Thus, the black seed and honey mixture at ratio 1:1 was the most potent anticancer agent with an IC50 value of 7.44 μg/mL. The present study illustrated that black seed and honey mixtures possess a lipid-lowering effect via LDL-c uptake in HepG2 cells (p<0.05). The highest LDL-c uptake was observed at 15 μg/mL with the treatment of black seed and honey mixture at 1:2 ratio which was 294.4%. Further studies should be conducted on primary human liver cells to further justify the correlation between the antioxidant level and LDLc uptake mechanism of black seed and honey mixtures.

Effect of Honey & Olive Oil Supplemented Bio-Yoghurt Feeding on Lipid Profile, Blood Glucose and Hematological Parameters in Rats

2019 ◽  
Vol 15 (2) ◽  
pp. 140-147
Author(s):  
Magdy M. Ismail ◽  
El-Tahra M. Ammar ◽  
Abd El-Wahab E. Khalil ◽  
Mohamed Z. Eid
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Blood Glucose ◽  
Olive Oil ◽  
Body Weight Gain ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol

Background and Objective: Yoghurt, especially bio-yoghurt has long been recognized as a product with many health benefits for consumers. Also, honey and olive oil have considerable nutritional and health effects. So, the effect of administration of yoghurt made using ABT culture and fortified with honey (2 and 6%), olive oil (1 and 4%) or honey + olive oil (2+1 and 6+4% respectively) on some biological and hematological properties of rats was investigated.Methods:The body weight gain, serum lipid level, blood glucose level, serum creatinine level, Glutamic Oxaloacetic Transaminase (GOT) activity, Glutamic Pyruvic Transaminase (GPT) activity, leukocytes and lymphocytes counts of rats were evaluated.Results:Blending of bio-yoghurt with rats&#039; diet improved body weight gain. Concentrations of Total plasma Cholesterol (TC), High-Density Lipoprotein cholesterol (HDL), Low-Density Lipoprotein cholesterol (LDL), Very Low-Density Lipoprotein cholesterol (VLDL) and Triglycerides (TG) significantly lowered in plasma of rats fed bio-yoghurt. Levels of TC, LDL, VLDL, and TG also decreased in rat groups feed bio-yoghurt supplemented with honey and olive oil. LDL concentrations were reduced by 10.32, 18.51, 34.17, 22.48, 43.30% in plasma of rats fed classic starter yoghurt, ABT yoghurt, ABT yoghurt contained 6% honey, ABT yoghurt contained 4% olive oil and ABT yoghurt contained 6% honey + 4% olive oil respectively. The blood glucose, serum creatinine, GOT and GPT values of rats decreased while white blood cells and lymphocytes counts increased by feeding bioyoghurt contained honey and olive oil.Conclusion:The findings enhanced the multiple therapeutic effects of bio-yoghurt supplemented with honey and olive oil.

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