Analysis of the insecticidal activity of methylisothiocyanate on Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) and its parasitoid Dinarmus basalis (Rondani) (Hymenoptera: Pteromalidae)

2002 ◽  
Vol 38 (2) ◽  
pp. 129-138 ◽  
Author(s):  
A. Sanon ◽  
M. Garba ◽  
J. Auger ◽  
J. Huignard
Keyword(s):  
Insecticidal Activity ◽  
Callosobruchus Maculatus ◽  
Dinarmus Basalis

scholarly journals Insecticidal and Antifungal Activities of Chemically-Characterized Essential Oils from the Leaves of Withania frutescens L.

Life ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 88
Author(s):  
El Moussaoui Abdelfattah ◽  
Allali Aimad ◽  
Mohammed Bourhia ◽  
Khalid Chebbac ◽  
Ahmad Mohammad Salamatullah ◽  
...  
Keyword(s):  
Insecticidal Activity ◽  
Mycelial Growth ◽  
Callosobruchus Maculatus ◽  
Mortality Rates ◽  
Inhibitory Effect ◽  
Gas Chromatography Mass Spectrometry ◽  
Contact Toxicity ◽  
Inhalation Toxicity ◽  
Phytochemical Constituents ◽  
Significant Mortality

The current study was conducted to investigate antifungal and insecticidal activities of essential oil extracted from the Moroccan Withania frutescens L. (EOW), and their chemical composition was profiled. To achieve this goal, EOW was extracted by the hydro-distillation method and their phytochemical constituents were characterized by gas chromatography-mass spectrometry analyses (GC-MS). Insecticidal activity was evaluated by use of four tests: contact toxicity, inhalation toxicity, and repellent tests. Antifungal activity was evaluated on Fusarium oxysporum f. sp. Ciceris (F. oxysporum) using different concentrations of EOW. GC/MS analysis revealed that EOW was rich in carvacrol (31.87%), thymol (30.08%), and camphor (9.13%). At a 1-µL/L dose, EOW exhibited mortality rates of 23.13 ± 1.07% and 24.41 ± 1.21% against Callosobruchus maculatus (C.maculatus) by inhalation and contact, respectively. Notably, EOW dose of 20 μL/L caused significant mortality rates of 95.1 ± 3.5% and 76.69 ± 1.71% by inhalation and contact, respectively. EOW exhibited an inhibitory effect on mycelial growth against the tested fungi F. oxysporum of 100% and 93.5 ± 1.1% for the 9 and 4.5 mg/mL doses, respectively. The reduced mycelial growth rate for F. oxysporum was recorded to be 0.3 ± 0.1 and 0.6 ± 0.1 mm/h for the EOW doses of 2.25 and 4.5 mg/mL, respectively. The outcome of the present work showed that EOW has a promising antifungal and insecticidal activity, and it can therefore be employed as a natural alternative insecticidal and mycocidal agent to replace the chemically-synthesized ones.

scholarly journals Competition in the Parasitization of Callosobruchus maculatus between Dinarmus basalis and Choetospila elegans on Vigna unguiculata (Walp.) Seeds

2012 ◽  
Vol 2 (1) ◽  
pp. 92
Author(s):  
Umoetok S. B. A. ◽  
Ukeh D. A. ◽  
Udo I. A. ◽  
Ekanem M. ◽  
Iloba B. N
Keyword(s):  
Vigna Unguiculata ◽  
Callosobruchus Maculatus ◽  
Pearson Correlation ◽  
Management Strategies ◽  
Negative Relationship ◽  
Positive Association ◽  
Storage Pests ◽  
Early Introduction ◽  
C Elegans ◽  
Dinarmus Basalis

<p>Laboratory study was carried out to investigate the efficacy of hymenopteran parasitoids, (<em>Dinarmus basalis</em> and <em>Choetospila elegans)</em> parasitization of <em>Callosobruchus maculatus</em> on cowpea (<em>Vigna unguiculata</em>) seeds in the Department of Crop Science, University of Calabar, Nigeria. Treatments were the inoculation of <em>D. basalis</em> (sole), <em>C. elegans</em> (sole), a mixture of <em>D. basalis</em> and <em>C. elegans</em> into infested cowpea seeds and the control which received no parasitoids. The introduction of parasitoids was repeated at 3 d interval, and all treatments were replicated 3 times. Result indicated that early introduction of the parasitoids caused significantly (P ? 0.05) higher mortality of <em>C.maculatus</em> than late introduction. Mix culture of both parasitoids was significantly more efficacious than sole culture. The emergence of each parasitoid and the mortality of <em>C. maculatus</em> in sole culture showed a highly significant (P ? 0.01) positive relationship (r = 0.487) between <em>C. maculatus</em> and <em>D. basalis</em> but non-significant (P ? 0.05) though positive association (r = 0.289) with <em>C. elegans</em>. A multiple regression analysis between <em>C. maculatus </em>mortality and the emergence of the two parasitoids in mix culture also indicated a highly positive significant (P ? 0.01) relationship (R = 0.751, R<sup>2</sup> = 0.564). Pearson Correlation analysis also showed a significant (P ? 0.05) positive association (r = 0.464, and 0.401) between <em>C. maculatus</em> mortality and emergence of <em>C. elegans</em> and <em>D. basalis</em> in mix culture, respectively. However, a non significant (P ? 0.05) negative relationship (r = -0.336) was observed between the two parasitoids in their emergence in mix culture, but <em>D. basalis</em> contributed more to the mortality of <em>C. maculatus</em> than <em>C. elegans</em>. Data from this study showed that <em>D. basalis</em> and <em>C. elegans</em> have potentials to be deployed as biological control entities in the management of <em>C. maculatus</em> as a part of the integrated pest management strategies of storage pests in the tropics.</p>

The efficacy of essential oil of Alpinia calcarata (Rosc.) and its major constituent, 1,8-cineole, as protectants of cowpea against Callosobruchus maculatus (F.) (Coleoptera: Bruchidae)

2006 ◽  
Vol 86 (3) ◽  
pp. 821-827 ◽  
Author(s):  
Krishanthi Abeywickrama ◽  
A. A. C. K. Adhikari ◽  
Priyani Paranagama ◽  
Chammi S. Palehepitiya Gamage
Keyword(s):  
Essential Oil ◽  
Insecticidal Activity ◽  
Callosobruchus Maculatus ◽  
Adult Emergence ◽  
The Other ◽  
Repellent Activity ◽  
Major Constituent ◽  
Fumigant Toxicity ◽  
Toxicity Assay ◽  
Cowpea Seeds

The bruchid, Callosobruchus maculatus, (F.) causes major losses during the storage of cowpea seeds [Vigna unguiculata (L.) Walp.] in Sri Lanka. Essential oil was extracted from Alpinia calcarata (Rosc.) and 1,8-cineole, identified as the major constituent of the essential oil, was tested for its potential insecticidal activity against C. maculatus. The adults of C. maculatus were susceptible to both fumigant toxicity and contact and fumigant toxicity of 1,8-cineole and A. calcarata oil. In the fumigant toxicity assay, the LC50 (0.685 g L-1) value of 1,8-cineole was similar to that of A. calcarata oil. However, LC50 values of 0.068 g L-1 and 0.141 g L-1 were obtained for the contact and fumigant toxicity of 1,8-cineole and A. calcarata oil, respectively. Oviposition and F1 adult emergence were significantly inhibited by 1,8-cineole and A. calcarata oil at concentrations higher than 0.80 g L-1 in the fumigant toxicity and 0.123 g L-1 in the contact plus fumigant toxicity. Repellent activity of A. calcarata oil was more potent to C. maculatus than 1,8-cineole at doses ranging from 10 to 40 mg. This study reveals that 1,8-cineole is the most toxic component present in A. calcarata oil, and that the repellent activity of A. calcarata oil could be due to 1,8-cineole as well as the other constituents present in the essential oil. Key words: Callosobruchus maculatus (F.), Alpinia calcarata (Rosc.), 1,8-cineole, essential oils, cowpea

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