The inhibitory effect of bovine rumen fluid on Salmonella typhimurium

1979 ◽  
Vol 26 (3) ◽  
pp. 273-276 ◽  
Author(s):  
P.G. Chambers ◽  
R.J. Lysons
Keyword(s):  
Salmonella Typhimurium ◽  
Rumen Fluid ◽  
Inhibitory Effect ◽  
Bovine Rumen

In Vitro Inactivation of Escherichia coli O157:H7 in Bovine Rumen Fluid by Caprylic Acid

2004 ◽  
Vol 67 (5) ◽  
pp. 884-888 ◽  
Author(s):  
THIRUNAVUKKARASU ANNAMALAI ◽  
MANOJ KUMAR MOHAN NAIR ◽  
PATRICK MAREK ◽  
PRADEEP VASUDEVAN ◽  
DAVID SCHREIBER ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Caprylic Acid ◽  
Rumen Fluid ◽  
Bacterial Load ◽  
Anaerobic Bacteria ◽  
Inhibitory Effect ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Bovine Rumen

The antibacterial effect of caprylic acid (35 and 50 mM) on Escherichia coli O157:H7 and total anaerobic bacteria at 39° C in rumen fluid (pH 5.6 and 6.8) from 12 beef cattle was investigated. The treatments containing caprylic acid at both pHs significantly reduced (P < 0.05) the population of E. coli O157:H7 compared with that in the control samples. At pH 5.6, both levels of caprylic acid killed E. coli O157:H7 rapidly, reducing the pathogen population to undetectable levels at 1 min of incubation (a more than 6.0-log CFU/ml reduction). In buffered rumen fluid at pH 6.8, 50 mM caprylic acid reduced the E. coli O157:H7 population to undetectable levels at 1 min of incubation, whereas 35 mM caprylic acid reduced the pathogen by approximately 3.0 and 5.0 log CFU/ml at 8 and 24 h of incubation, respectively. At both pHs, caprylic acid had a significantly lesser (P < 0.05) and minimal inhibitory effect on the population of total anaerobic bacteria in rumen compared with that on E. coli O157:H7. At 24 h of incubation, caprylic acid (35 and 50 mM) reduced the population of total anaerobic bacteria by approximately 2.0 log CFU/ml at pH 5.6, whereas at pH 6.8, caprylic acid (35 mM) did not have any significant (P > 0.05) inhibitory effect on total bacterial load. Results of this study revealed that caprylic acid was effective in inactivating E. coli O157:H7 in bovine rumen fluid, thereby justifying its potential as a preslaughter dietary supplement for reducing pathogen carriage in cattle.

Comparison of Antimutagenic Effect of Various Tea Extracts (Green, Oolong, Pouchong, and Black Tea)

1994 ◽  
Vol 57 (1) ◽  
pp. 54-58 ◽  
Author(s):  
GOW-CHIN YEN ◽  
HUI-YIN CHEN
Keyword(s):  
Salmonella Typhimurium ◽  
Green Tea ◽  
Aflatoxin B1 ◽  
Black Tea ◽  
Inhibitory Effect ◽  
Manufacturing Processes ◽  
Antimutagenic Effect ◽  
Oolong Tea ◽  
Tea Extract

The antimutagenic effects of various tea extracts prepared from nonfermented tea (green tea), semifermented tea (oolong tea and pouchong tea), and fermented tea (black tea) were investigated by Salmonella/microsome assay. No mutagenicity or toxicity in Salmonella typhimurium TA98 and TA100 was observed with any tea extract. The tea extracts markedly inhibited the mutagenicity of 2-amino-3-methylimidazo(4,5-f)quinoline, 3-amino-1,4-dimethyl-5H-pyridol(4,3-b)indole,2-amino-6-methyldipyrido(l,2-a:3′,2′-d)imidazole, benzo[a]pyrene, and aflatoxin B1 toward S. typhimurium TA98 and TA100 in the presence of S9 mixture, especially those of oolong and pouchong teas inhibited over 90% mutagenicity of these five mutagens at the dosage of 1 mg per plate. Among four tea extracts, black tea exhibited the weakest inhibitory effect on mutagenicity of these five mutagens. The mutagenicity of 4-nitroquinoline-N-oxide, a direct mutagen, was not inhibited by black and oolong tea extracts to S. typhimurium TA98 in the absence of S9 mixture but was increased by the tea extracts at the dose of 1 mg per plate to S. typhimurium TA100. As the antimutagenic effect of semifermented tea was stronger than nonfermented and fermented teas, some antimutagenic substances might be formed during manufacturing processes of tea.

In Vitro Metabolism of Formononetin and Biochanin a in Bovine Rumen Fluid

1988 ◽  
Vol 66 (8) ◽  
pp. 1969 ◽  
Author(s):  
J. M. Dickinson ◽  
G. R. Smith ◽  
R. D. Randel ◽  
I. J. Pemberton
Keyword(s):  
Rumen Fluid ◽  
In Vitro Metabolism ◽  
Biochanin A ◽  
Bovine Rumen

Inhibitory effect of cold nitrogen plasma on Salmonella Typhimurium biofilm and its application on poultry egg preservation

LWT ◽  
2020 ◽  
Vol 126 ◽  
pp. 109340 ◽  
Author(s):  
Lin Lin ◽  
Xue Liao ◽  
Changzhu Li ◽  
Mohamed A. Abdel-Samie ◽  
Haiying Cui
Keyword(s):  
Salmonella Typhimurium ◽  
Inhibitory Effect ◽  
Nitrogen Plasma

Inhibitory effect of sour orange (Citrus aurantium) juice on Salmonella Typhimurium and Listeria monocytogenes

LWT ◽  
2014 ◽  
Vol 55 (2) ◽  
pp. 421-425 ◽  
Author(s):  
Şeniz Karabıyıklı ◽  
Hüseyin Değirmenci ◽  
Mehmet Karapınar
Keyword(s):  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Inhibitory Effect ◽  
Sour Orange ◽  
Citrus Aurantium

scholarly journals Clostridium lundense sp. nov., a novel anaerobic lipolytic bacterium isolated from bovine rumen

2006 ◽  
Vol 56 (3) ◽  
pp. 625-628 ◽  
Author(s):  
Dores G. Cirne ◽  
Osvaldo D. Delgado ◽  
Sankar Marichamy ◽  
Bo Mattiasson
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Rumen Fluid ◽  
Sequence Similarity ◽  
Carbon Sources ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Bovine Rumen ◽  
Hybridization Data ◽  
Clostridium Tetanomorphum

A strictly anaerobic, mesophilic, endospore-forming, lipolytic bacterium, designated strain R1T, was isolated from bovine rumen fluid and characterized. Cells of this isolate were Gram-positive, non-motile rods that formed spherical terminal spores. The overall biochemical and physiological characteristics indicated that this strain should be placed in the genus Clostridium. The strain grew at temperatures between 25 and 47 °C (optimum, 37 °C), at pH between 5·0 and 8·5 (optimum pH 5·5–7·0) and in NaCl concentrations of 0–3 % (w/v). The isolate was not able to utilize glucose or other carbohydrates as carbon sources. The DNA G+C content was 31·2 mol%. Sequence analysis of the 16S rRNA gene of R1T revealed that it has the closest match (98 % similarity) with Clostridium tetanomorphum DSM 4474T. The highest levels of DNA–DNA relatedness of the isolate were 61·9 and 54·3 % with Clostridium pascui DSM 10365T and C. tetanomorphum DSM 4474T, respectively. Based on 16S rRNA gene sequence similarity, phylogenetic analysis, DNA G+C content, DNA–DNA hybridization data and distinct phenotypic characteristics, strain R1T (=DSM 17049T=CCUG 50446T) was classified in the genus Clostridium, as a member of a novel species, for which the name Clostridium lundense sp. nov. is proposed.

Effects of heavy metals and other trace elements on the fermentative activity of the rumen microflora and growth of functionally important rumen bacteria

1978 ◽  
Vol 24 (3) ◽  
pp. 298-306 ◽  
Author(s):  
C. W. Forsberg
Keyword(s):  
Trace Elements ◽  
Rumen Fluid ◽  
Inhibitory Effect ◽  
Rumen Bacteria ◽  
Inhibitory Effects ◽  
Butyrivibrio Fibrisolvens ◽  
Fermentative Activity ◽  
Rumen Microflora ◽  
High Concentrations

The inhibitory effects of high concentrations of essential and non-essential trace elements were tested on the rumen microflora using the rate of fermentation in vitro as the assay. The elements (and the concentration causing 50% inhibition) in decreasing order of toxicity were Hg2+ (20 μg/ml), Cu2+ (21 μg/ml), Cr6+ (70 μg/ml), Se4+ (73 μg/ml), Ni2+ (160 μg/ml), Cd2+ (175 μg/ml), As3+ (304 μg/ml), and As5+ (1610 μg/ml). The elements tested that were either weak or non-inhibitory at concentrations greater than 400 μg/ml included Zn2+, Cr2+, Fe2+, Mn2+, Pb2+, and Co2+. Methylmercury was as inhibitory as mercuric chloride to the fermentation. When the inhibitory effect of Cd2+ was tested on separated bacterial and protozoal fractions, it was more inhibitory to the bacteria. The inhibitory effects of trace elements were also determined for a number of axenic cultures of rumen bacteria. The bacteria which most frequently exhibited the greatest sensitivity were Bacteroides succinogenes, Ruminococcus albus, Bacteroides amytophilus, and Eubacterium ruminantium. Those often exhibiting intermediate sensitivities included Butyrivibrio fibrisolvens, Selenomonas niminantium, and Megasphera elsdenii, while Streptococcus bovis was very refractory to all elements tested. Rumen fluid provided a modest protective effect for the bacteria.

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