Toxicity and immunogenicity of Crotalus durissus terrificus venom treated with different doses of gamma rays

Toxicon ◽  
1999 ◽  
Vol 37 (8) ◽  
pp. 1131-1141 ◽  
Author(s):  
Patricia Bianca Clissa ◽  
Nanci do Nascimento ◽  
José Roberto Rogero
Keyword(s):  
Gamma Rays ◽  
Crotalus Durissus Terrificus ◽  
Crotalus Durissus ◽  
Different Doses

L-aminoacid oxidase from Crotalus durissus terrificus snake venom and its antibacterial potential

Toxicon ◽  
2019 ◽  
Vol 168 ◽  
pp. S13
Author(s):  
Leonardo Melo ◽  
Lidiane Nunes Barbosa ◽  
Rui Seabra Ferreira Junior ◽  
Benedito Barraviera ◽  
Luciana Curtolo De Barros ◽  
...  
Keyword(s):  
Snake Venom ◽  
Crotalus Durissus Terrificus ◽  
Crotalus Durissus ◽  
Antibacterial Potential

scholarly journals Multiple effects of toxins isolated from Crotalus durissus terrificus on the hepatitis C virus life cycle

PLoS ONE ◽  
2017 ◽  
Vol 12 (11) ◽  
pp. e0187857 ◽  
Author(s):  
Jacqueline Farinha Shimizu ◽  
Carina Machado Pereira ◽  
Cintia Bittar ◽  
Mariana Nogueira Batista ◽  
Guilherme Rodrigues Fernandes Campos ◽  
...  
Keyword(s):  
Life Cycle ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Crotalus Durissus Terrificus ◽  
Virus Life Cycle ◽  
Crotalus Durissus

Semen collection and evaluation in free-ranging Brazilian rattlesnakes (Crotalus durissus terrificus)

Zoo Biology ◽  
2007 ◽  
Vol 26 (2) ◽  
pp. 155-160 ◽  
Author(s):  
Rogério Loesch Zacariotti ◽  
Kathleen Fernandes Grego ◽  
Wilson Fernandes ◽  
Sávio Stefanini Sant'Anna ◽  
Marcelo Alcindo de Barros Vaz Guimarães
Keyword(s):  
Crotalus Durissus Terrificus ◽  
Semen Collection ◽  
Crotalus Durissus ◽  
Free Ranging

scholarly journals Inhibition of Neurotoxic Secretory Phospholipases A2Enzymatic, Edematogenic, and Myotoxic Activities by Harpalycin 2, an Isoflavone Isolated fromHarpalyce brasilianaBenth

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Rafael M. Ximenes ◽  
Marcelo M. Rabello ◽  
Renata M. Araújo ◽  
Edilberto R. Silveira ◽  
Fábio H. R. Fagundes ◽  
...  
Keyword(s):  
Active Site ◽  
Initial Step ◽  
Significant Inhibition ◽  
Lipid Mediators ◽  
Naja Naja ◽  
Crotalus Durissus Terrificus ◽  
Docking Calculations ◽  
Crotalus Durissus ◽  
Bothrops Pirajai ◽  
Phospholipases A

Secretory phospholipases A2(sPLA2) exert proinflammatory actions through lipid mediators. These enzymes have been found to be elevated in many inflammatory disorders such as rheumatoid arthritis, sepsis, and atherosclerosis. The aim of this study was to evaluate the effect of harpalycin 2 (Har2), an isoflavone isolated fromHarpalyce brasilianaBenth., in the enzymatic, edematogenic, and myotoxic activities of sPLA2fromBothrops pirajai, Crotalus durissus terrificus, Apis mellifera,andNaja najavenoms. Har2 inhibits all sPLA2tested. PrTX-III (B. pirajaivenom) was inhibited at about 58.7%, Cdt F15 (C. d. terrificusvenom) at 78.8%, Apis (from bee venom) at 87.7%, and Naja (N. najavenom) at 88.1%. Edema induced by exogenous sPLA2administration performed in mice paws showed significant inhibition by Har2 at the initial step. In addition, Har2 also inhibited the myotoxic activity of these sPLA2s. In order to understand how Har2 interacts with these enzymes, docking calculations were made, indicating that the residues His48 and Asp49 in the active site of these enzymes interacted powerfully with Har2 through hydrogen bonds. These data pointed to a possible anti-inflammatory activity of Har2 through sPLA2inhibition.

scholarly journals Thermolability of 28S ribosomal ribonucleic acid from the liver of Crotalus durissus terrificus (Ophidia, Reptilia)

1973 ◽  
Vol 135 (1) ◽  
pp. 73-79 ◽  
Author(s):  
J. F. Giorgini ◽  
F. L. De Lucca
Keyword(s):  
Molecular Weight ◽  
Gradient Centrifugation ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sucrose Density Gradient ◽  
Dimethyl Sulphoxide ◽  
Low Molecular Weight ◽  
28S Rrna ◽  
Sucrose Density Gradient Centrifugation ◽  
Crotalus Durissus Terrificus ◽  
Crotalus Durissus

Instability of 28S rRNA of Crotalus durissus terrificus liver was observed during hotphenol extraction: purified 28S rRNA is converted into an 18S RNA component by heat treatment. It was also found that ‘6S’ and ‘8S’ low-molecular-weight RNA species were released during the thermal conversion. This conversion and the release of the low-molecular-weight species were also induced by 8m-urea and 80% (v/v) dimethyl sulphoxide at 0°C. Evidence is presented that this phenomenon is an irreversible process and results from the rupture of hydrogen bonds. The 18S RNA product was shown to be homogeneous by polyacrylamide-gel electrophoresis and by sucrose-density-gradient centrifugation. The base composition of the 18S RNA products obtained by heat, urea or dimethyl sulphoxide treatments was similar. The C+G content of the 18S RNA product was different from that of the native 18S rRNA, but similar to that of 28S rRNA.

scholarly journals Crotoxin-Induced Mice Lung Impairment: Role of Nicotinic Acetylcholine Receptors and COX-Derived Prostanoids

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 794
Author(s):  
Marco Aurelio Sartim ◽  
Camila O. S. Souza ◽  
Cassiano Ricardo A. F. Diniz ◽  
Vanessa M. B. da Fonseca ◽  
Lucas O. Sousa ◽  
...  
Keyword(s):  
Respiratory Failure ◽  
Early Phase ◽  
Late Phase ◽  
Lung Mechanics ◽  
Respiratory Compromise ◽  
Nicotinic Acetylcholine ◽  
Crotalus Durissus Terrificus ◽  
Crotalus Durissus ◽  
The Impact ◽  
Lung Impairment

Respiratory compromise in Crotalus durissus terrificus (C.d.t.) snakebite is an important pathological condition. Considering that crotoxin (CTX), a phospholipase A2 from C.d.t. venom, is the main component of the venom, the present work investigated the toxin effects on respiratory failure. Lung mechanics, morphology and soluble markers were evaluated from Swiss male mice, and mechanism determined using drugs/inhibitors of eicosanoids biosynthesis pathway and autonomic nervous system. Acute respiratory failure was observed, with an early phase (within 2 h) characterized by enhanced presence of eicosanoids, including prostaglandin E2, that accounted for the increased vascular permeability in the lung. The alterations of early phase were inhibited by indomethacin. The late phase (peaked 12 h) was marked by neutrophil infiltration, presence of pro-inflammatory cytokines/chemokines, and morphological alterations characterized by alveolar septal thickening and bronchoconstriction. In addition, lung mechanical function was impaired, with decreased lung compliance and inspiratory capacity. Hexamethonium, a nicotinic acetylcholine receptor antagonist, hampered late phase damages indicating that CTX-induced lung impairment could be associated with cholinergic transmission. The findings reported herein highlight the impact of CTX on respiratory compromise, and introduce the use of nicotinic blockers and prostanoids biosynthesis inhibitors as possible symptomatic therapy to Crotalus durissus terrificus snakebite.

Sign in / Sign up

Export Citation Format

Share Document