Citrate as an in vivo marker to discriminate prostate cancer from benign prostatic hyperplasia and normal prostate peripheral zone: Detection via localized proton spectroscopy

Urology ◽  
1995 ◽  
Vol 45 (3) ◽  
pp. 459-466 ◽  
Author(s):  
John Kurhanewicz ◽  
Daniel B. Vigneron ◽  
Sarah J. Nelson ◽  
Hedvig Hricak ◽  
Jeffrey M. MacDonald ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Benign Prostatic Hyperplasia ◽  
Peripheral Zone ◽  
Prostatic Hyperplasia ◽  
Proton Spectroscopy ◽  
Normal Prostate

scholarly journals Ki-67 and PCNA expression in prostate cancer and benign prostatic hyperplasia

2008 ◽  
Vol 31 (1) ◽  
pp. 8 ◽  
Author(s):  
WeiDe Zhong ◽  
Jinyu Peng ◽  
HuiChan He ◽  
Dinglan Wu ◽  
ZhaoDong Han ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Benign Prostatic Hyperplasia ◽  
Early Diagnosis ◽  
Prostatic Hyperplasia ◽  
Nuclear Antigen ◽  
Growth Fraction ◽  
Resting Cells ◽  
Ki 67 ◽  
Normal Prostate ◽  
Expression Levels

Objective: Ki-67 is a proliferation-associated nuclear antigen and is expressed in all cycling cells except for resting cells in the G0-phase. PCNA is an acidic nuclear protein and has been recognized as a histologic marker for the G1/S phase in the cell cycle. Ki-67and PCNA labeling indices are considered to reflect cell proliferation, particularly, growth fraction. The purpose of this study is to investigate the expression levels of Ki-67 and PCNA in prostate cancer (PCa) and benign prostatic hyperplasia (BPH) and their potential on the early diagnosis of PCa. Methods: Human prostate cancer cell lines LNCaP and PC-3, human normal prostate epithelial cell line HuPEC, tissues from patients with PCa (121 cases) and BPH (45) and 36 normal cases were examined for the expression of Ki-67 and PCNA by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Then, the association of Ki-67 and PCNA expression with clinical grading of PCa was analyzed by immunohistochemistry staining. Results: The ratios of PCNA and Ki-67 expression levels in LNCaP and PC-3 were higher (P < 0.05, P < 0.001) than that in HuPEC. The two markers were differentially expressed in three tissues and showed increased expression in PCa (P < 0.05) and BPH (P < 0.05), relative to human normal prostate tissues. Compared with BPH, the ratio of Ki-67 and PCNA expressed in tumour tissue was increased (P < 0.05). The increase of Ki-67 was greater than that of PCNA. Expression of the two markers increased after different grading of PCa cases. The values of Ki-67/PCNA were: 0.073 in grade I PCa tissues, 0.119 in grade IIa PCa tissues, 0.141 in grade IIa PCa tissues, 0.234 in grade III PCa tissues. Conclusion: The combination of Ki-67 and PCNA, specific proliferative markers of PCa, may improve the accuracy of early diagnosis of prostatic cancer.

In Vivo Quantification of Water Diffusion, Stiffness, and Tissue Fluidity in Benign Prostatic Hyperplasia and Prostate Cancer

2020 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Patrick Asbach ◽  
Sa-Ra Ro ◽  
Nader Aldoj ◽  
Joachim Snellings ◽  
Rolf Reiter ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Benign Prostatic Hyperplasia ◽  
Water Diffusion ◽  
Prostatic Hyperplasia

scholarly journals Pomegranate Extracts in the Management of Men's Urologic Health: Scientific Rationale and Preclinical and Clinical Data

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Kroeger N. ◽  
Belldegrun A. S. ◽  
Pantuck A. J.
Keyword(s):  
Prostate Cancer ◽  
Erectile Dysfunction ◽  
Benign Prostatic Hyperplasia ◽  
Fruits And Vegetables ◽  
Evidence Base ◽  
Prostatic Hyperplasia ◽  
Urological Diseases ◽  
Scientific Rationale

Multiple strands of research provide growing evidence that diet, nutrition, and life style play a role in the development and the course of urological diseases. Numerous micronutrients and polyphenols found in soy, green tea, and many fruits and vegetables have been described to impact diseases including erectile dysfunction, benign prostatic hyperplasia, and prostate cancer. However, oftentimes these reports lack both a scientific rationale and supportive evidence base. The efficacy of pomegranate, on the other hand, in the modulation of central biological processes like inflammation, hypoxia, and oxidative stress that are important in the pathogenesis of urological maladies has been robustly demonstrated in preclinicalin vitroandin vivostudies. Moreover, clinical trials have further supported its use in the treatment of several diseases, in particular in the management of prostate cancer. Herein, we critically review the scientific knowledge about the current role and future prospects for the use of pomegranate extracts in the therapy of erectile dysfunction, benign prostatic hyperplasia, and prostate cancer.

Accumulation of [ 11 C]acetate in normal prostate and benign prostatic hyperplasia: comparison with prostate cancer

2002 ◽  
Vol 29 (11) ◽  
pp. 1492-1495 ◽  
Author(s):  
Takashi Kato ◽  
Eriko Tsukamoto ◽  
Yuji Kuge ◽  
Toshiki Takei ◽  
Tohru Shiga ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Benign Prostatic Hyperplasia ◽  
Prostatic Hyperplasia ◽  
Normal Prostate

scholarly journals A System for Studying Epithelial-Stromal Interactions Reveals Distinct Inductive Abilities of Stromal Cells from Benign Prostatic Hyperplasia and Prostate Cancer

Endocrinology ◽  
2005 ◽  
Vol 146 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Wendy W. Barclay ◽  
Ralph D. Woodruff ◽  
M. Craig Hall ◽  
Scott D. Cramer
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Epithelial Cell ◽  
Efficient Method ◽  
Stromal Cells ◽  
Peripheral Zone ◽  
Renal Tissue ◽  
Prostatic Hyperplasia ◽  
Epithelial Cell Line ◽  
Blue Dye

The development of normal and abnormal glandular structures in the prostate is controlled at the endocrine and paracrine levels by reciprocal interactions between epithelium and stroma. To study these processes, it is useful to have an efficient method of tissue acquisition for reproducible isolation of cells from defined histologies. Here we assessed the utility of a standardized system for acquisition and growth of prostatic cells from different regions of the prostate with different pathologies, and we compared the abilities of stromal cells from normal peripheral zone, benign prostatic hyperplasia (BPH-S), and cancer to induce the growth of a human prostatic epithelial cell line (BPH-1) in vivo. Using the tissue recombination method, we showed that grafting stromal cells (from any histology) alone or BPH-1 epithelial cells alone produced no visible grafts. Recombining stromal cells from normal peripheral zone with BPH-1 cells also produced no visible grafts (n = 15). Recombining BPH-S with BPH-1 cells generated small, well-organized, and sharply demarcated grafts approximately 3–4 mm in diameter (n = 9), demonstrating a moderate inductive ability of BPH-S. Recombining stromal cells from cancer with BPH-1 cells generated highly disorganized grafts that completely surrounded the host kidney and invaded into adjacent renal tissue, demonstrating induction of an aggressive phenotype. We conclude that acquisition of tissue from toluidine blue dye-stained specimens is an efficient method to generate high-quality epithelial and/or stromal cultures. Stromal cells derived by this method from areas of BPH and cancer induce epithelial cell growth in vivo, which mimics the natural history of these diseases.

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