A System for Studying Epithelial-Stromal Interactions Reveals Distinct Inductive Abilities of Stromal Cells from Benign Prostatic Hyperplasia and Prostate Cancer

The development of normal and abnormal glandular structures in the prostate is controlled at the endocrine and paracrine levels by reciprocal interactions between epithelium and stroma. To study these processes, it is useful to have an efficient method of tissue acquisition for reproducible isolation of cells from defined histologies. Here we assessed the utility of a standardized system for acquisition and growth of prostatic cells from different regions of the prostate with different pathologies, and we compared the abilities of stromal cells from normal peripheral zone, benign prostatic hyperplasia (BPH-S), and cancer to induce the growth of a human prostatic epithelial cell line (BPH-1) in vivo. Using the tissue recombination method, we showed that grafting stromal cells (from any histology) alone or BPH-1 epithelial cells alone produced no visible grafts. Recombining stromal cells from normal peripheral zone with BPH-1 cells also produced no visible grafts (n = 15). Recombining BPH-S with BPH-1 cells generated small, well-organized, and sharply demarcated grafts approximately 3–4 mm in diameter (n = 9), demonstrating a moderate inductive ability of BPH-S. Recombining stromal cells from cancer with BPH-1 cells generated highly disorganized grafts that completely surrounded the host kidney and invaded into adjacent renal tissue, demonstrating induction of an aggressive phenotype. We conclude that acquisition of tissue from toluidine blue dye-stained specimens is an efficient method to generate high-quality epithelial and/or stromal cultures. Stromal cells derived by this method from areas of BPH and cancer induce epithelial cell growth in vivo, which mimics the natural history of these diseases.

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Benign prostatic hyperplasia (BPH) epithelial cell line BPH-1 induces aromatase expression in prostatic stromal cells via prostaglandin E2

Journal of Endocrinology ◽

10.1677/joe-06-0181 ◽

2007 ◽

Vol 195 (1) ◽

pp. 89-94 ◽

Cited By ~ 13

Author(s):

Quan Wu ◽

Ying Zhou ◽

Linfeng Chen ◽

Jiandang Shi ◽

Chun-Yu Wang ◽

...

Keyword(s):

Prostate Cancer ◽

Benign Prostatic Hyperplasia ◽

Epithelial Cell ◽

Stromal Cells ◽

Prostate Cancer Cell ◽

Prostatic Hyperplasia ◽

Epithelial Cell Line ◽

Prostate Cancer Cell Lines ◽

Aromatase Expression ◽

Cox 2

Estradiol (E2) level in stroma of benign prostatic hyperplasia (BPH) increases with age, and this increase was associated with an elevated expression of aromatase in prostatic stromal cells (PrSCs). Here, we showed that conditioned medium (CM) of BPH-1 (a benign hyperplastic prostatic epithelial cell line), but not of prostate cancer cell lines (LNCaP, DU-145, and PC-3), stimulates aromatase expression in PrSCs. Cyclooxygenase-2 (COX-2) mRNA level in BPH-1, as well as prostaglandin E2 (PGE2) concentration in BPH-1 CM, was significantly higher than that of prostate cancer cell lines. CM of BPH-1 treated with NS-398 (a specific inhibitor of COX-2) failed to stimulate aromatase expression in PrSCs. And PGE2 can stimulate aromatase expression in PrSCs. Our data suggested that BPH-1 induced aromatase expression in PrSCs through the production of PGE2 in a paracrine mechanism.

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Citrate as an in vivo marker to discriminate prostate cancer from benign prostatic hyperplasia and normal prostate peripheral zone: Detection via localized proton spectroscopy

Urology ◽

10.1016/s0090-4295(99)80016-8 ◽

1995 ◽

Vol 45 (3) ◽

pp. 459-466 ◽

Cited By ~ 157

Author(s):

John Kurhanewicz ◽

Daniel B. Vigneron ◽

Sarah J. Nelson ◽

Hedvig Hricak ◽

Jeffrey M. MacDonald ◽

...

Keyword(s):

Prostate Cancer ◽

Benign Prostatic Hyperplasia ◽

Peripheral Zone ◽

Prostatic Hyperplasia ◽

Proton Spectroscopy ◽

Normal Prostate

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Inflammatory Responses in a Benign Prostatic Hyperplasia Epithelial Cell Line (BPH-1) Infected with Trichomonas vaginalis

The Korean Journal of Parasitology ◽

10.3347/kjp.2016.54.2.123 ◽

2016 ◽

Vol 54 (2) ◽

pp. 123-132 ◽

Cited By ~ 10

Author(s):

Sang-Su Kim ◽

Jung-Hyun Kim ◽

Ik-Hwan Han ◽

Myoung-Hee Ahn ◽

Jae-Sook Ryu

Keyword(s):

Benign Prostatic Hyperplasia ◽

Epithelial Cell ◽

Cell Line ◽

Trichomonas Vaginalis ◽

Inflammatory Responses ◽

Prostatic Hyperplasia ◽

Epithelial Cell Line

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Zone-Dependent Expression of Estrogen Receptors α and β in Human Benign Prostatic Hyperplasia

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jc.2002-021015 ◽

2003 ◽

Vol 88 (3) ◽

pp. 1333-1340 ◽

Cited By ~ 60

Author(s):

Toshifumi Tsurusaki ◽

Daiyu Aoki ◽

Hiroshi Kanetake ◽

Satoshi Inoue ◽

Masami Muramatsu ◽

...

Keyword(s):

Prostate Cancer ◽

In Situ Hybridization ◽

Benign Prostatic Hyperplasia ◽

Estrogen Receptors ◽

Stromal Cells ◽

Crucial Role ◽

Peripheral Zone ◽

Prostatic Hyperplasia ◽

Cellular Distribution

Estrogen, which acts through estrogen receptors (ERs) α and β, has been implicated in the pathogenesis of benign and malignant human prostatic tumors, i.e. benign prostatic hyperplasia and prostate cancer, thought to originate from different zones of the prostate [the transition zone (TZ) and peripheral zone (PZ), respectively]. Here, we examined the cellular distribution of ERα and ERβ in human normal and hyperplastic prostate tissues, using in situ hybridization and immunohistochemistry. ERα expression was restricted to stromal cells of PZ. In contrast, ERβ was expressed in the stromal cells of PZ as well as TZ. ERβ-positive epithelial cells were evenly distributed in PZ and TZ of the prostate. Our results suggest that estrogen may play a crucial role in the pathogenesis of benign prostatic hyperplasia through ERβ.

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Resveratrol induces apoptosis of benign prostatic hyperplasia epithelial cell line (BPH-1) through p38 MAPK-FOXO3a pathway

BMC Complementary and Alternative Medicine ◽

10.1186/s12906-019-2648-8 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 5

Author(s):

Chao Li ◽

Wan-Li Hu ◽

Meng-Xin Lu ◽

Guan-Fa Xiao

Keyword(s):

Benign Prostatic Hyperplasia ◽

Epithelial Cell ◽

Cell Line ◽

P38 Mapk ◽

Prostatic Hyperplasia ◽

Epithelial Cell Line

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Three-dimensional Growth of Renal Epithelial Cells in Vitro: A Tool in Toxicity Testing

Alternatives to Laboratory Animals ◽

10.1177/026119299302100212 ◽

1993 ◽

Vol 21 (2) ◽

pp. 191-195 ◽

Cited By ~ 1

Author(s):

Knut-Jan Andersen ◽

Erik Ilsø Christensen ◽

Hogne Vik

Keyword(s):

Epithelial Cells ◽

Three Dimensional ◽

Toxicity Testing ◽

Renal Tissue ◽

Epithelial Cell Line ◽

Multicellular Spheroids ◽

Renal Epithelial Cell ◽

Renal Epithelial Cells

The tissue culture of multicellular spheroids from the renal epithelial cell line LLC-PK1 (proximal tubule) is described. This represents a biological system of intermediate complexity between renal tissue in vivo and simple monolayer cultures. The multicellular structures, which show many similarities to kidney tubules in vivo, including a vectorial water transport, should prove useful for studying the potential nephrotoxicity of drugs and chemicals in vitro. In addition, the propagation of renal epithelial cells as multicellular spheroids in serum-free culture may provide information on the release of specific biological parameters, which may be suppressed or masked in serum-supplemented media.

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Electrophysiological Measurements of a Toad Renal Epithelial Cell Line (A6) as an Assay for Predicting Ocular Eye Irritancy

Alternatives to Laboratory Animals ◽

10.1177/026119299202000206 ◽

1992 ◽

Vol 20 (2) ◽

pp. 218-221

Author(s):

Henning F. Bjerregaard

Keyword(s):

Epithelial Cell ◽

Cell Line ◽

South African ◽

Mdck Cells ◽

Epithelial Cell Line ◽

Renal Epithelial Cell ◽

Electrophysiological Measurements ◽

Renal Epithelial Cell Line ◽

Darby Canine Kidney

An established epithelial cell line (A6) from a South African clawed toad (Xenopus laevis) kidney was used as a model for the corneal epithelium of the eye in order to determine ocular irritancy. When grown on Millipore filter inserts, A6 cells form a monolayer epithelium of high electrical resistance and generate a trans-epithelial potential difference. These two easily-measured electrophysiological endpoints showed a dose-related decrease after exposure for 24 hours to seven selected chemicals of different ocular irritancy potential. It was demonstrated that both trans-epithelial resistance and potential ranked closely with in vivo eye irritancy data and correlated well (r = 0.96) with loss of trans-epithelial impermeability of Madin-Darby canine kidney (MDCK) cells, detected by use of a fluorescein leakage assay.

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