IDENTIFICATION OF HUMAN T AND B LYMPHOCYTES IN NORMAL PERIPHERAL BLOOD AND IN CHRONIC LYMPHOCYTIC LEUKÆMIA

Background:Wnt signalling has recently been implicated in the pathogenesis of cancer.Methods:This study investigated the activity of Wnt signalling in peripheral blood chronic lymphocytic leukaemia (CLL) lymphocytes, and the methylation status of seven soluble Wnt antagonist genes, including WIF1, DKK3, APC, SFRP1, SFRP2, SFRP4 and SFRP5, by using methylation-specific PCR in the peripheral blood CLL lymphocytes and bone marrow samples of patients with CLL at diagnosis.Results:In the peripheral blood CLL lymphocytes, constitutive activation of Wnt signalling was detected, associated with hypermethylation of the soluble Wnt inhibitor genes. In the diagnostic CLL marrow samples, methylation of the seven genes was detected in up to 36.4% of samples. Moreover, 23 (52.3%) patients had methylation of at least one of the seven genes, of whom 14 (60.8%) had methylation of two or more Wnt inhibitor genes. Apart from an association of advanced age with DKK3 methylation, there was no association of gene hypermethylation with either clinical characteristics (including age, gender, lymphocyte count at diagnosis, Rai stage and poor-risk karyotype) or survival.Conclusion:Wnt signalling is constitutively activated in CLL B lymphocytes in association with methylation of multiple soluble Wnt antagonist genes. Methylation of these soluble Wnt antagonist genes, occasionally multiple genes, in primary CLL marrow samples suggests an important role in CLL pathogenesis. Moreover, this study underscored the importance of studying methylation of a panel of, but not individual, genes regulating a cellular pathway.

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Induction of B-cell chronic lymphocytic leukaemia and hairy cell leukaemia like phenotypes by phorbol ester treatment of normal peripheral blood B-cells

British Journal of Haematology ◽

10.1111/j.1365-2141.1990.tb04349.x ◽

1990 ◽

Vol 75 (3) ◽

pp. 359-365 ◽

Cited By ~ 12

Author(s):

Lydia Visser ◽

Sibrand Poppema

Keyword(s):

B Cells ◽

Chronic Lymphocytic Leukaemia ◽

B Cell ◽

Phorbol Ester ◽

Peripheral Blood ◽

Lymphocytic Leukaemia ◽

Cell Leukaemia ◽

Hairy Cell ◽

Normal Peripheral Blood ◽

Cell Chronic Lymphocytic Leukaemia

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The Local Sanarelli-Shwartzman Phenomenon in Rats Induced by Human Leukaemic Cells

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647777 ◽

1973 ◽

Vol 29 (02) ◽

pp. 353-362

Author(s):

J Lisiewicz ◽

A Pituch ◽

J. A Litwin

Keyword(s):

Chronic Lymphocytic Leukaemia ◽

Intravenous Injection ◽

Peripheral Blood ◽

Lymphocytic Leukaemia ◽

Acute Myeloblastic Leukaemia ◽

Demarcation Line ◽

E Coli ◽

Leukaemic Cells ◽

Shwartzman Phenomenon

SummaryThe local Sanarelli-Shwartzman phenomenon (SSP-L) in the skin of 30 rats was induced by an intr a cutaneous sensitizing injection of leukaemic leucocytes isolated from the peripheral blood of patients with chronic lymphocytic leukaemia (CLL), acute myeloblastic leukaemia (AL) and chronic granulocytic leukaemia (CGL) and challenged by an intravenous injection of 100(μ of E. coli endotoxin. SSP-L was observed in 7 rats after injection of CLL lymphocytes and in 6 and 2 rats after AL myeloblasts and the CGL granulocytes, respectively. The lesions in the skin after AL myeloblasts appeared in a shorter time and were of longer duration compared with those observed after CLL lymphocytes and CGL granulocytes. Histologically, the lesions consisted of areas of destruction in the superficial layers of the skin ; the demarcation line showed the presence of neutrophils, macrophages and erythrocytes. Haemorrhages and fibrin deposits near the demarcation line were larger after injection of CLL lymphocytes and AL myeloblasts than after CGL granulocytes. The possible role of leucocyte procoagulative substances in the differences observed have been discussed.

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