Dicyanopyrazine studies. Syntheses and characterization of new bis-styryl fluorescent dyes from DAMN. Part III

1998 ◽  
Vol 36 (4) ◽  
pp. 395-405 ◽  
Author(s):  
Jae-yun Jaung ◽  
Masaru Matsuoka ◽  
Koushi Fukunishi
Keyword(s):  
Fluorescent Dyes

Characterization of Push–Pull-Type Benzo[X]quinoline Derivatives (X = g or f): Environmentally Responsive Fluorescent Dyes with Multiple Functions

2020 ◽  
Vol 85 (20) ◽  
pp. 13177-13190
Author(s):  
Yasufumi Fuchi ◽  
Tomohiro Umeno ◽  
Yuichiro Abe ◽  
Keita Ikeno ◽  
Ryu Yamasaki ◽  
...  
Keyword(s):  
Fluorescent Dyes ◽  
Quinoline Derivatives ◽  
Multiple Functions ◽  
Environmentally Responsive

scholarly journals Acidocalcisomes and Polyphosphate Granules Are Different Subcellular Structures in Agrobacterium tumefaciens

2020 ◽  
Vol 86 (8) ◽  
Author(s):  
Celina Frank ◽  
Dieter Jendrossek
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Fluorescent Dyes ◽  
Fluorescent Protein ◽  
Ralstonia Eutropha ◽  
Yellow Fluorescent Protein ◽  
Eukaryotic Cells ◽  
Enhanced Yellow Fluorescent Protein ◽  
Content Type ◽  
Polyphosphate Granules

ABSTRACT Acidocalcisomes are membrane-enclosed, polyphosphate-containing acidic organelles in lower Eukaryota but have also been described for Agrobacterium tumefaciens (M. Seufferheld, M. Vieira, A. Ruiz, C. O. Rodrigues, S. Moreno, and R. Docampo, J Biol Chem 278:29971–29978, 2003, https://doi.org/10.1074/jbc.M304548200). This study aimed at the characterization of polyphosphate-containing acidocalcisomes in this alphaproteobacterium. Unexpectedly, fluorescence microscopic investigation of A. tumefaciens cells using fluorescent dyes and localization of constructed fusions of polyphosphate kinases (PPKs) and of vacuolar H+-translocating pyrophosphatase (HppA) with enhanced yellow fluorescent protein (eYFP) suggested that acidocalcisomes and polyphosphate are different subcellular structures. Acidocalcisomes and polyphosphate granules were frequently located close together, near the cell poles. However, they never shared the same position. Mutant strains of A. tumefaciens with deletions of both ppk genes (Δppk1 Δppk2) were unable to form polyphosphate but still showed cell pole-located eYFP-HppA foci and could be stained with MitoTracker. In conclusion, A. tumefaciens forms polyP granules that are free of a surrounding membrane and thus resemble polyP granules of Ralstonia eutropha and other bacteria. The composition, contents, and function of the subcellular structures that are stainable with MitoTracker and harbor eYFP-HppA remain unclear. IMPORTANCE The uptake of alphaproteobacterium-like cells by ancestors of eukaryotic cells and subsequent conversion of these alphaproteobacterium-like cells to mitochondria are thought to be key steps in the evolution of the first eukaryotic cells. The identification of acidocalcisomes in two alphaproteobacterial species some years ago and the presence of homologs of the vacuolar proton-translocating pyrophosphatase HppA, a marker protein of the acidocalcisome membrane in eukaryotes, in virtually all species within the alphaproteobacteria suggest that eukaryotic acidocalcisomes might also originate from related structures in ancestors of alphaproteobacterial species. Accordingly, alphaproteobacterial acidocalcisomes and eukaryotic acidocalcisomes should have similar features. Since hardly any information is available on bacterial acidocalcisomes, this study aimed at the characterization of organelle-like structures in alphaproteobacterial cells, with A. tumefaciens as an example.

Synthesis and Characterization of Ratiometric, pH Sensing Nanoparticles with Covalently Attached Fluorescent Dyes

2006 ◽  
Vol 18 (15) ◽  
pp. 3381-3384 ◽  
Author(s):  
Honghao Sun ◽  
Anne Marie Scharff-Poulsen ◽  
Hong Gu ◽  
Kristoffer Almdal
Keyword(s):  
Fluorescent Dyes ◽  
Synthesis And Characterization ◽  
Ph Sensing ◽  
Ratiometric Ph Sensing

scholarly journals Europium-Doped Ceria Nanocrystals as Nanozyme Fluorescent Probes for Biosensing

2021 ◽  
Vol 5 (1) ◽  
pp. 53
Author(s):  
Ali Othman ◽  
Akhtar Hayat ◽  
Silvana Andreescu
Keyword(s):  
Fluorescent Dyes ◽  
Aqueous Media ◽  
Doped Ceria ◽  
New Wave ◽  
Lactate Oxidase ◽  
Fluorescence Sensing ◽  
Combined Use ◽  
Direct Fluorescence ◽  
Average Size

Molecular nanoprobes with intrinsic enzyme-like activity represent a new wave of technology for rapid and sensitive detection of molecular targets. This work reports synthesis and characterization of novel and well-dispersed europium-doped ceria nanocrystals (EuCe NCs) with self-integrated catalytic and fluorescence sensing functions. The NCs have an average size of ∼5 nm and exhibit bright and stable fluorescence for more than 6 months in aqueous media. Their dual cooperative function as both a catalyst and fluorescent probe was explored to develop a universally applicable fluorescence-based biosensing method to monitor enzyme reactions and quantitatively measure clinically relevant molecules. Sensing capabilities are demonstrated for detection of H2O2, glucose/glucose oxidase, lactate/lactate oxidase, phosphatase activity, and the catecholamine neurotransmitter, dopamine. Results indicate that EuCe NCs not only provide high enzyme-mimetic activity, but also impart direct fluorescence sensing ability enabling all-in-one recognition, catalytic amplification, and the detection of biomolecular targets. The EuCe nanozyme offers a stable alternative to the more complex systems based on the combined use of natural enzymes and fluorescent dyes. The high stability and fluorescence detection capabilities demonstrate that EuCe NCs have the potential to be used as a generic platform in chemical and biological sensing and bioimaging applications.

scholarly journals ANALISIS KONEKTIVITAS DAN KARAKTERISTIK LORONG PADA SISTEM HIDROGEOLOGI MATAAIR BETON, KAWASAN KARST GUNUNGSEWU, KABUPATEN GUNUNGKIDUL DENGAN UJI PERUNUTAN

2020 ◽  
Vol 12 (02) ◽  
pp. 105 ◽  
Author(s):  
Ahmad Cahyadi ◽  
Eko Haryono ◽  
Tjahyo Nugroho Adji ◽  
M Widyastuti ◽  
Muhammad Naufal ◽  
...  
Keyword(s):  
Catchment Area ◽  
Fluorescent Dyes ◽  
Groundwater Vulnerability ◽  
Tracer Test ◽  
Karst Area ◽  
Irrigated Rice ◽  
Karst Areas ◽  
Further Development ◽  
Hydrogeological System

Pemahaman tentang sistem hidrogeologi dan wilayah tangkapan air dari sebuah mata air sangatlah penting. Hal ini untuk membantu pengelolaan yang menjaga kelestariannya. Mataair Beton merupakan salah satu mataair yang memiliki peranan yang sangat penting di wilayah Ponjong, Kabupaten Gunungkidul. Suplai air dari mataair ini digunakan untuk memenuhi kebutuhan air bersih, irigasi persawahan dan perikanan. Penelitian ini bertujuan untuk mengetahui konektivitas dan karakterisasi lorong di sistem hidrogeologi Mataair Beton. Metode yang digunakan adalah tracer test dengan menggunakan fluorescent dyes. Hasil analisis menunjukkan bahwa Ponor Seropan memiliki konektivitas dengan Mataair Beton, dan memiliki karakteristik lorong berupa single conduit. Perkembangan lorong yang lanjut menunjukkan bahwa sistem hidrogeologi di lokasi kajian sangat dipengaruhi oleh imbuhan airtanah dari sistem alogenik yang berhulu di wilayah non-karst dan memiliki kerentanan terhadap pencemaran airtanah yang tinggi.Kata Kunci: Karst, Sungai Alogenik, Kawasan Karst Gunung Sewu, Uji Perunutan, Mataair BetonAn understanding of the hydrogeological system and catchment area of spring is very important. This is to help manage that maintains its sustainability. Beton Resurgence is one of the springs that have a very important role in the Ponjong area, Gunungkidul Regency. Water supply from the spring is used to supply clean water, irrigated rice fields, and fisheries. This study aims to determine the connectivity and characterization of Passage in the Beton hydrogeological system. The method used is a tracer test using fluorescent dyes. The results of the analysis of breaktrough curve indicate that Seropan Sinking Stream has connectivity with Beton resurgence, and has passage characteristics in the form of a single conduit. The further development of the passage shows that the hydrogeological system is strongly influenced by groundwater recharge originating from an allogenic system that originates in non-karst areas and has a high groundwater vulnerability to pollution. Keywords: Karst, Allogenic River, Gunungsewu Karst Area, Tracer Test, Beton Resurgence

scholarly journals Fabrication and Characterization of a Low-Cost Microfluidic System for the Manufacture of Alginate–Lacasse Microcapsules

Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1158 ◽  
Author(s):  
Ana Lucia Campaña ◽  
Diana Camila Sotelo ◽  
Hector Alfonso Oliva ◽  
Andres Aranguren ◽  
Nancy Ornelas-Soto ◽  
...  
Keyword(s):  
Fluorescent Dyes ◽  
Low Cost ◽  
Microfluidic System ◽  
Universal Serial Bus ◽  
Final Particle ◽  
Pycnoporus Sanguineus ◽  
Fluid Behavior ◽  
Multiphysics Simulations ◽  
Capsule Size

The development of microfluidics-based systems in the recent years has provided a rapid and controlled method for the generation of monodisperse microencapsulates for multiple applications. Here, we explore the design, manufacture and characterization of a low-cost microsystem for the encapsulation of the fungal laccase from Pycnoporus sanguineus CS43 in alginate microcapsules. Multiphysics simulations were used to overview the fluid behavior within the device and estimate the resulting capsule size. Polymethylmethacrylate (PMMA) sheets were used for final microsystem manufacture. Different flow rates of the continuous (Qc) and discrete (Qd) phases in the ranges of 83–293 mL/h and 1–5 mL/h, respectively, were evaluated for microcapsule fabrication. Universal Serial Bus (USB) microscope and image analysis was used to measure the final particle size. Laccase encapsulation was evaluated using spectrophotometry and with the aid of fluorescent dyes and confocal microscopy. Results showed microcapsule size was in the range of 203.13–716.00 μm and Qc was found as the dominant parameter to control capsule size. There was an effective enzyme encapsulation of 65.94% with respect to the initial laccase solution.

Sign in / Sign up

Export Citation Format

Share Document