Procedure 31 Rapid electrochemical verification of PCR amplification of Salmonella spp. based on m-GEC electrodes

2007 ◽  
pp. e221-e226 ◽  
Author(s):  
María Isabel Pividori ◽  
Anabel Lermo ◽  
Susana Campoy ◽  
Jordi Barbé ◽  
Salvador Alegret
Keyword(s):  
Pcr Amplification ◽  
Salmonella Spp

scholarly journals Rapid Detection of Salmonella spp. by PCR Amplification of Salmonella Specific Region in gatD gene.

1999 ◽  
Vol 16 (2) ◽  
pp. 99-109 ◽  
Author(s):  
Takahisa MIYAMOTO ◽  
Sudsai TREVANICH ◽  
Ken-ichi HONJOH ◽  
Shoji HATANO
Keyword(s):  
Rapid Detection ◽  
Pcr Amplification ◽  
Specific Region ◽  
Salmonella Spp

scholarly journals A Simple Gold Nanoprobe Assay for the Identification of Staphylococcus Aureus, Listeria Monocytogenes and Salmonella Enteritidis in Food Specimens

2017 ◽  
Vol 6 (4) ◽  
pp. 134 ◽  
Author(s):  
Dimitra Panagiotis Houhoula ◽  
Ekatherina Charvalos ◽  
Spyros Konteles ◽  
Stamatis Koussissis ◽  
Vladimiros Lougovois ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Listeria Monocytogenes ◽  
Dna Sequences ◽  
Salmonella Enteritidis ◽  
Screening Method ◽  
Pcr Amplification ◽  
Reference Method ◽  
Direct Pcr ◽  
Salmonella Spp ◽  
Gold Nanoprobe

In the present study, we developed a gold nanoprobe assay, which relies on the colorimetric differentiation of specific DNA sequences, based on different aggregation profiles. We evaluated the assay on DNA extracted from pathogen cultures and from contaminated food specimens. The targets used were the femA gene for the identification of Staphylococcus aureus, the hly gene of the Listeria monocytogenes listeriolysin and the invA sequence for Salmonella spp. Comparison was performed with the reference assay, as described in the respective ISO guidelines for each pathogen, and a direct PCR amplification and detection. The minimum detection limit of the assay was defined at 123 fg/μL DNA, for all species, lower than PCR detection. Specificity was 100%. Sensitivity was 100%, as compared το the reference method, whereas the sensitivity of PCR was 93.3%. The evaluated assay could be used as a sensitive screening method, which does not require major infrastructure, for the detection and identification of pathogens in food specimens. In addition, it can accommodate detection of multiplespecies,thus allowing multiplexingand expedite turnaround time. 

scholarly journals Prevalence and Relative Risk of Cronobacter spp., Salmonella spp., and Listeria monocytogenes Associated with the Body Surfaces and Guts of Individual Filth Flies

2012 ◽  
Vol 78 (22) ◽  
pp. 7891-7902 ◽  
Author(s):  
Monica Pava-Ripoll ◽  
Rachel E. Goeriz Pearson ◽  
Amy K. Miller ◽  
George C. Ziobro
Keyword(s):  
Listeria Monocytogenes ◽  
Relative Risk ◽  
Urban Areas ◽  
Pcr Amplification ◽  
Body Part ◽  
The Body ◽  
Salmonella Spp ◽  
Content Type ◽  
Food Borne Pathogens ◽  
Food Borne

ABSTRACTAlthough flies are important vectors of food-borne pathogens, there is little information to accurately assess the food-related health risk of the presence of individual flies, especially in urban areas. This study quantifies the prevalence and the relative risk of food-borne pathogens associated with the body surfaces and guts of individual wild flies. One hundred flies were collected from the dumpsters of 10 randomly selected urban restaurants. Flies were identified using taxonomic keys before being individually dissected.Cronobacterspp.,Salmonellaspp., andListeria monocytogeneswere detected using the PCR-based BAX system Q7. Positive samples were confirmed by culture on specific media and through PCR amplification and sequencing or ribotyping. Among collected flies were the housefly,Musca domestica(47%), the blowflies,Lucilia cuprina(33%) andLucilia sericata(14%), and others (6%).Cronobacterspecies were detected in 14% of flies, includingC. sakazakii,C. turicensis, andC. universalis, leading to the proposal of flies as a natural reservoir of this food-borne pathogen. Six percent of flies carriedSalmonella enterica, including the serovars Poona, Hadar, Schwarzengrund, Senftenberg, and Brackenridge.L. monocytogeneswas detected in 3% of flies. Overall, the prevalence of food-borne pathogens was three times greater in the guts than on the body surfaces of the flies. The relative risk of flies carrying any of the three pathogens was associated with the type of pathogen, the body part of the fly, and the ambient temperature. These data enhance the ability to predict the microbiological risk associated with the presence of individual flies in food and food facilities.

Characterization of a cDNA for Rhesus Monkey Protein C Inhibitor – Evidence for N-Terminal Involvement in Heparin Stimulation

1995 ◽  
Vol 74 (04) ◽  
pp. 1079-1087 ◽  
Author(s):  
Klaus-P Radtke ◽  
José A Fernández ◽  
Bruno O Villoutreix ◽  
Judith S Greengard ◽  
John H Griffin
Keyword(s):  
Rhesus Monkey ◽  
Protein C ◽  
Activated Protein C ◽  
Pcr Amplification ◽  
Amino Acid Sequences ◽  
Heparin Binding ◽  
Reactive Center ◽  
Protein C Inhibitor ◽  
Polymerase Chain

SummarycDNAs for protein C inhibitor (PCI) were cloned from human and rhesus monkey 1 liver RNAs by reverse transcription and polymerase chain reaction (PCR) amplification. Sequencing showed that rhesus monkey and human PCI cDNAs were 93% identical. Predicted amino acid sequences differed at 26 of 387 residues. Pour of these differences (T352M, N359S, R362K, L3631) were in the reactive center loop that is important for inhibitory specificity, and two were in the N-terminal helix (M8T, E13K) that is implicated in glycosaminoglycan binding. PCI in human or rhesus monkey plasma showed comparable inhibitory activity towards human activated protein C in the presence of 10 U/ml heparin. However, maximal acceleration of the inhibition of activated protein C required 5-fold lower heparin concentration for rhesus monkey than for human plasma, consistent with the interpretation that the additional positive charge (E13K) in a putative-heparin binding region increased the affinity for heparin.

Simple and Rapid Detection of Factor V Leiden by Allele-specific PCR Amplification

1996 ◽  
Vol 75 (05) ◽  
pp. 757-759 ◽  
Author(s):  
Rainer Blasczyk ◽  
Markus Ritter ◽  
Christian Thiede ◽  
Jenny Wehling ◽  
Günter Hintz ◽  
...  
Keyword(s):  
Direct Sequencing ◽  
Activated Protein C ◽  
Factor V Leiden ◽  
Pcr Amplification ◽  
Factor V ◽  
Specific Pcr ◽  
Pcr Rflp ◽  
Allele Specific ◽  
Specific Amplification ◽  
Allele Specific Pcr

SummaryResistance to activated protein C is the most common hereditary cause for thrombosis and significantly linked to factor V Leiden. In this study, primers were designed to identify the factor V mutation by allele-specific PCR amplification. 126 patients with thromboembolic events were analysed using this technique, PCR-RFLP and direct sequencing. The concordance between these techniques was 100%. In 27 patients a heterozygous factor VGln506 mutation was detected, whereas one patient with recurrent thromboembolism was homozygous for the point mutation. Due to its time- and cost-saving features allele-specific amplification should be considered for screening of factor VGln506.

EVALUATION OF THE ISOLATION AND DETECTION METHODS FOR SALMONELLA SPP. FROM EGG SHELL CONTAMINATION USING MULTIPLEX PCR.

2015 ◽  
Vol 14 (1) ◽  
pp. 282-288
Author(s):  
Israa Adnan Ibraheam Al-Baghdady ◽  
Ashwak Bassim Jassim ◽  
Zainab Khudher Ahmed
Keyword(s):  
Multiplex Pcr ◽  
Detection Methods ◽  
Salmonella Spp ◽  
Egg Shell

ISOLATION OF SALMONELLA SPP. FROM SLAUGHTERED SHEEP IN BASRA

2012 ◽  
Vol 11 (2) ◽  
pp. 159-166
Author(s):  
Wessam M. Mohammed Saleh
Keyword(s):  
Salmonella Spp

Quintuplex PCR to Detect Antibiotic Resistance Genes in Streptococcus pneumoniae

2016 ◽  
Vol 1 (2) ◽  
pp. 22 ◽  
Author(s):  
Navindra Kumari Palanisamy ◽  
Parasakthi Navaratnam ◽  
Shamala Devi Sekaran
Keyword(s):  
Antibiotic Resistance ◽  
Streptococcus Pneumoniae ◽  
Resistance Genes ◽  
Bacterial Pathogen ◽  
Antibiotic Resistance Genes ◽  
Pcr Amplification ◽  
Penicillin Resistance ◽  
Penicillin Binding Proteins ◽  
Efflux Mechanism ◽  
Mic Value

Introduction: Streptococcus pneumoniae is an important bacterial pathogen, causing respiratory infection. Penicillin resistance in S. pneumoniae is associated with alterations in the penicillin binding proteins, while resistance to macrolides is conferred either by the modification of the ribosomal target site or efflux mechanism. This study aimed to characterize S. pneumoniae and its antibiotic resistance genes using 2 sets of multiplex PCRs. Methods: A quintuplex and triplex PCR was used to characterize the pbp1A, ermB, gyrA, ply, and the mefE genes. Fifty-eight penicillin sensitive strains (PSSP), 36 penicillin intermediate strains (PISP) and 26 penicillin resistance strains (PRSP) were used. Results: Alteration in pbp1A was only observed in PISP and PRSP strains, while PCR amplification of the ermB or mefE was observed only in strains with reduced susceptibility to erythromycin. The assay was found to be sensitive as simulated blood cultures showed the lowest level of detection to be 10cfu. Conclusions: As predicted, the assay was able to differentiate penicillin susceptible from the non-susceptible strains based on the detection of the pbp1A gene, which correlated with the MIC value of the strains.

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