Cloning of cDNA encoding a soybean allergen, Gly m Bd 28K

2001 ◽  
Vol 1518 (1-2) ◽  
pp. 178-182 ◽  
Author(s):  
Hideaki Tsuji ◽  
Miki Hiemori ◽  
Masumi Kimoto ◽  
Hiromi Yamashita ◽  
Ryoko Kobatake ◽  
...  
Keyword(s):  
Soybean Allergen

scholarly journals Levels of Airborne Soybean Allergen (Gly m 1) in a Brazilian Soybean Production City: A Pilot Study

2020 ◽  
Vol 17 (15) ◽  
pp. 5381
Author(s):  
Cinthya Covessi Thom de Souza ◽  
Nelson Augusto Rosário Filho ◽  
Juliana Francis de Camargo ◽  
Ricardo Henrique Moreton Godoi
Keyword(s):  
Pilot Study ◽  
Immunosorbent Assay ◽  
Detection Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Soybean Production ◽  
Soy Production ◽  
Assay Detection ◽  
Soybean Allergen

Asthma epidemics have been shown to be related to where soybeans are loaded and handled, but data are scarce in the literature. This pilot study evaluated the levels of Gly m 1 in dust samples collected in Maringá, Brazil, a city with high soy production and processing. A dust impactor was used to collect seven isolated samples during 2015 and 2016. Samples were analyzed by an ELISA (enzyme-linked immunosorbent assay) detection method. Gly m 1 was found in all samples, ranging from 0.82–24.38 ng/m3 (median 2.41), regardless of the month or year evaluated. The levels of Gly m 1 were considered low, but the concentrations required to cause sensitization and symptoms are uncertain.

scholarly journals Analysis of the IgE epitope profile of soybean allergen Gly m 4

2014 ◽  
Vol 4 (S2) ◽  
Author(s):  
Felix Husslik ◽  
Diana Mittag ◽  
Christian Seutter von Loetzen ◽  
Maximilian Hartl ◽  
Lothar Vogel ◽  
...  
Keyword(s):  
Ige Epitope ◽  
Soybean Allergen

Fructooligosaccharide Inhibits the Absorption of β-conglycinin (A Major Soybean Allergen) in IPEC-J2

2018 ◽  
Vol 15 (1-2) ◽  
Author(s):  
Yuan Zhao ◽  
Shiyao Zhang ◽  
Xiaodong Zhang ◽  
Li Pan ◽  
Nan Bao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Enzymatic Hydrolysis ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Time Dependent ◽  
Dependent Manner ◽  
Intestinal Epithelial ◽  
Intracellular Accumulation ◽  
Novel Therapeutic ◽  
Soybean Allergen

AbstractDissecting the inhibited variation of allergen absorption could contribute to the development of novel therapeutic or preventive treatments for food/feed allergies. This study investigated the effects of fructooligosaccharide (FOS) on the absorption, intracellular accumulation of intact or hydrolysed β-conglycinin in porcine intestinal epithelial cells (IPEC-J2). As demonstrated by ELISA and immunoblotting, β-conglycinin was absorbed in a dose- and time-dependent manner (p < 0.05). Actually, β-conglycinin was easily transported and absorbed after enzymatic hydrolysis. Three peptides (52 kDa, 30 kDa and 25 kDa) were produced during transcellular absorption of intact or hydrolysed β-conglycinin. FOS inhibited the absorption of β-conglycinin, especially the 52 and 30 kDa peptides. The immunoreactive peptides derived from the 52, 35 or 22 kDa peptides were the substrings of the known epitopes determined by mass spectrometry and bioinformatic analyses. These results indicate that FOS can efficiently inhibit the absorption of 52 and 30 kDa peptides derived from β-conglycinin.

Occurrence of IgE Antibody-Recognizing N-Linked Glycan Moiety of a Soybean Allergen, Gly m Bd 28K

2000 ◽  
Vol 122 (4) ◽  
pp. 238-245 ◽  
Author(s):  
Miki Hiemori ◽  
Noriko Bando ◽  
Tadashi Ogawa ◽  
Hisao Shimada ◽  
Hideaki Tsuji ◽  
...  
Keyword(s):  
Ige Antibody ◽  
Soybean Allergen

scholarly journals Degradation and removal of soybean allergen in Japanese soy sauce

2017 ◽  
Vol 16 (2) ◽  
pp. 2264-2268 ◽  
Author(s):  
Norihiro Magishi ◽  
Naoya Yuikawa ◽  
Makio Kobayashi ◽  
Shoichiro Taniuchi
Keyword(s):  
Soy Sauce ◽  
Soybean Allergen

Micro-assay Method for Evaluating the Allergenicity of the Major Soybean Allergen,Gly mBd 30K, with Mouse Antiserum and RBL-2H3 Cells

1997 ◽  
Vol 61 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Rintaro Yamanishi ◽  
Hideaki Tsuji ◽  
Noriko Bando ◽  
Izumi Yoshimoto ◽  
Tadashi Ogawa
Keyword(s):  
Assay Method ◽  
Soybean Allergen

Identification of an IgE epitope of soybean allergen Gly m Bd 60K

LWT ◽  
2020 ◽  
Vol 133 ◽  
pp. 110131 ◽  
Author(s):  
MengXue He ◽  
Jun Xi
Keyword(s):  
Ige Epitope ◽  
Soybean Allergen

scholarly journals Analysis of Gly m Bd 28K, Major Soybean Allergen, in Several Leading Varieties in Japan.

2001 ◽  
Vol 48 (5) ◽  
pp. 344-348 ◽  
Author(s):  
Ryota KANEGAE ◽  
Akio OBATA ◽  
Ryoichi MATSUNAGA ◽  
Masakazu TAKASHI ◽  
Kunihiko KOMATSU
Keyword(s):  
Soybean Allergen
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