PO-1273 Title: Impact of HPV infection in anal carcinoma patients treated with definitive chemoradiation

412 Background: Squamous cell anal carcinoma (SCAC) treatment remains unchanged since the institution of chemoradiation over 4 decades ago. Epidermal growth factor receptor (EGFR) is a protein often expressed in aggressive cancers and that is the target of the monoclonal antibody: cetuximab. Concurrent cetuximab and radiation has been particularly effective treating squamous cell carcinoma of the head and neck. Like head and neck cancer, anal cancer is an epithelial tumor of the alimentary tract that is radioresponsive and is associated with HPV infection. The rarity of this cancer limits its evaluation for biological markers. This study set out to thoroughly characterize EGFR expression by immunohistochemistry in 101 invasive SCAC tissue samples. Methods: One hundred and one pretreatment paraffin embedded invasive SCAC biopsies, obtained from the Montreal area between 1999 and 2009, were tested for EGFR expression by immunohistochemistry. All samples were confirmed to harbor invasive anal carcinoma on H&E slide preparations. Corresponding cancerous areas were identified on paraffin tissue blocks and cut out for tissue microarray analysis. Samples were immunostained with an EGFR antibody (clone SPM 341) on the Discovery XT Autostainer (Ventana), and staining was assessed by light microscopy by two pathologists. A semiquantitative combination score combining staining intensity with the percent of cells staining gave a final score: just detectable or weak (1+); moderate (2+); strong/intense (3+). Results: Of 101 patient biopsies, 82 samples had sufficient material for interpretation. Of these samples, 72/82 (90%) stained positive for EGFR, while 41/82 (50%) samples displayed at least moderate to strong staining. Conclusions: This is the largest cohort of SCAC tissue samples tested to date for EGFR expression and it confirms that the vast majority of invasive SCAC overexpress EGFR. EGFR likely plays a role in anal cancer tumor-genesis and progression. Testing of EGFR inhibitors in this patient population is justified. No significant financial relationships to disclose.

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Survival prediction models for patients with anal carcinoma receiving definitive chemoradiation: A population‑based study

Oncology Letters ◽

10.3892/ol.2019.11238 ◽

2019 ◽

Cited By ~ 2

Author(s):

Yinhang Wu ◽

Xiaoyang Han ◽

Yan Li ◽

Kunli Zhu ◽

Jinming Yu

Keyword(s):

Prediction Models ◽

Anal Carcinoma ◽

Population Based ◽

Survival Prediction ◽

Population Based Study ◽

Definitive Chemoradiation

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Anal Carcinoma: A Series of Cases with a Literature Review

Acta Facultatis Medicae Naissensis ◽

10.1515/afmnai-2016-0031 ◽

2016 ◽

Vol 33 (4) ◽

pp. 295-306

Author(s):

Momčilo Stošić ◽

Igor Stojanović ◽

Svetlana Mihajlović ◽

Kosta Zdravković

Keyword(s):

Surgical Treatment ◽

Literature Review ◽

Precancerous Lesions ◽

Anal Carcinoma ◽

Hpv Infection ◽

Review Of The Literature ◽

High Similarity ◽

Anal Adenocarcinoma ◽

Number Of Patients ◽

Digestive Cancers

Abstract The aim of this study was to analyze the cases of five patients with squamous cell carcinoma (SCC) and to draw attention to the dilemma in the treatment of incontinence on admission of these patients and to present, through a literature review, locations of the surgical treatment. The anal carcinoma is a rare disease which involves different histopathological (HP) types of cancer. They account for about 2.5% of all digestive cancers. The treatment of the disease is multimodal. There are dilemmas about screening of precancerous lesions and treatment of diagnosed incontinence. There is a high similarity between HPV infection and SCC. Our methods and results were compared with the data from the literature. In the period of three years (from 2013 to 2015), five cases of the anal carcinoma were diagnosed and treated in the regional hospital. In all of our cases, the disease was histopathologically confirmed as SCC of the anal and perianal areas. It excluded the cases of anal adenocarcinoma (AAC). Nearly half of the patients (40%) were incontinent and the rectum excision was one of the possible solutions. Distinction in histopathological structure created the confusion in the treatment of these patients. The dilemma we had was whether to do an amputation of the rectum in patients with incontinence before the standardized Chemoradiation therapy (CRT). The review of the literature sums up certain conclusions. The diagnosis of SCC includes the perianal "ulcer" that does not heal, as well as atypical anoperianal growths. Surgical treatment of SCC is indicated when the diameter of lesion is small (2 cm), or the disease is persistent or recurrent. In the case of anal incontinence because of the tumor changes, the abdominoperineal resection of the rectum (APR) is an alternative to performing bipolar colostomy and CRT. The review of the literature using MEDLINE/PubMed databases did not find the case with restoring anal continence after this treatment. Testing a larger number of patients with incontinence on admission would provide a more clear answer to the question of the treatment of these patients.

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External validation of leukocytosis and neutrophilia as a prognostic marker in anal carcinoma treated with definitive chemoradiation

Radiotherapy and Oncology ◽

10.1016/j.radonc.2017.06.009 ◽

2017 ◽

Vol 124 (1) ◽

pp. 110-117 ◽

Cited By ~ 6

Author(s):

Antoine Schernberg ◽

Florence Huguet ◽

Laurence Moureau-Zabotto ◽

Cyrus Chargari ◽

Eleonor Rivin Del Campo ◽

...

Keyword(s):

Prognostic Marker ◽

External Validation ◽

Anal Carcinoma ◽

Definitive Chemoradiation

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A combined ultrastructural and gene molecular research for the relationship between human uterine cervical carcinoma and human papillomavirus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010015856x ◽

1990 ◽

Vol 48 (3) ◽

pp. 204-205

Author(s):

Kun Lee ◽

Jingyi Si ◽

Ricai Han ◽

Wei Zhang ◽

Bingbing Tan ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Intraepithelial Neoplasia ◽

Hpv Infection ◽

Dot Blot ◽

Homologous Sequences ◽

Normal Cervical Epithelium ◽

The Relationship ◽

Chronic Cervicitis

There are more supports for the view that human papillomavirus (HPV) infection might be an etiological factor in the development of cervical cancer when the association of persistent condylomata is considered. Biopsies from 318 cases with squamous cell carcinoma of uterine cervix, 48 with cervical and vulvar condylomata, 14 with cervical intraepithelial neoplasia (CIN), 34 with chronic cervicitis and 24 normal cervical epithelium were collected from 5 geographic regions of China with different cervical cancer mortalities. All specimens were prepared for Dot blot, Southern blot and in situ DNA-DNA hybridizations by using HPV-11, 16, 18 DNA labelled with 32P and 3H as probes to detect viral homologous sequences in samples. Among them, 32 cases with cervical cancer, 27 with condyloma and 10 normal cervical epitheliums were randomly chosen for comparative EM observation. The results showed that: 1), 192 out of 318 (60.4%) cases of cervical cancer were positive for HPV-16 DNA probe (Table I)

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