562 TUMOR NECROSIS FACTOR-ALPHA PROMOTER GENE POLYMORPHISMS AFFECT THE OCCURRENCE OF HEPATOCELLULAR CARCINOMA IN PATIENTS WITH CHRONIC HEPATITIS B

Introduction: data about influence of intradermal vaccination with native autoleukocytes on activity level of pro-inflammatory cytokine tumor necrosis factor alpha in patients with chronic hepatitis B have been presented in the article. The aim: Based on positive results, obtained from autoleukocyte immunization in patients with psoriasis [14], the aim of our research was to use and study such therapy for reducing the synthesis of pro-inflammatory cytokine TNF-α in patients with chronic hepatitis B (chronic hepatitis B). Materials and methods: Patients with chronic hepatitis B with high level of tumor necrosis factor alpha (≥30pg/ml) were vaccinated with native autoleukocytes (23); simultaneously, the same procedure was performed to patients (11) with low level of this cytokine (5pg/ml). Leukocytes were isolated from heparinized peripheral venous blood of a patient with hepatitis B by centrifuging plasma, obtained after blood precipitation for 140-160 minutes at temperature 370 С. The suspension was resuspended in 1-1.5 ml of a patient’s blood serum and injected into the skin of the back in the dose 0.1 ml. Results: in 30 days after immunization, reduction of tumor necrosis factor alpha was observed in all patients with its high level (100%), in 65.25% of individuals – to 5 pg/ml; in some patients, who had low or average level of pro-inflammatory cytokine, the level individually increased (41.67%). Conclusions: The elaborated method of influence on activity of tumor necrosis factor alpha in patients with chronic hepatitis B is effective and worth implementing into clinical practice.

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Association of tumor necrosis factor alpha -238G/A and -308G/A promotor polymorphisms with clearance of Hepatitis B virus infection in Turkish population

Acta Medica ◽

10.32552/2019.actamedica.341 ◽

2019 ◽

Vol 50 (1) ◽

pp. 1-6

Author(s):

Ahmet Çağkan İnkaya ◽

Emel Türk Arıbaş ◽

İbrahim Erayman ◽

Hasan Acar ◽

Bahar Kandemir ◽

...

Keyword(s):

Chronic Hepatitis ◽

Tumor Necrosis Factor ◽

Hepatitis B ◽

Chronic Hepatitis B ◽

Tumor Necrosis Factor Alpha ◽

Tumor Necrosis ◽

Natural Immunity ◽

Necrosis Factor Alpha ◽

Factor Alpha ◽

Necrosis Factor

Aim: Acute viral hepatitis B may lead to chronic hepatitis in 6% of adult population. We compared the frequency of Tumor necrosis factor alpha promotor polymorphisms in chronic hepatitis B patients and people with natural immunity against hepatitis B. Material and Methodology: Chronic hepatitis B patients and age matched control cases with natural immunity to hepatitis B virus were recruited 1:1 in this study. Tumor necrosis factor alpha -238G/A and -308G/A polymorphisms were studied with PCR-RFLP. χ2 test was performed in statistical analysis. Results: A total of 101 volunteers enrolled in two study groups. Thirty-eight men and 12 women constituted the chronic hepatitis B patient group and 40 men and 11 women recruited in natural immunity group. Frequency of -238G allele was 87.5% and 97% in chronic hepatitis B and natural immunity groups, respectively. Frequency of -308G allel was 93% and 92.1% in chronic hepatitis B and natural immunity groups, respectively. Frequencies of polymorphisms at positions -238 and -308 in the promotor of tumor necrosis factor alpha gene were not different between chronic hepatitis B and natural immunity groups. Conclusion: Tumor necrosis factor alpha promoter polymorphisms at -238 and -308 positions do not effect the outcome hepatitis B infection in Turkish population. Clearance of hepatitis B virus infection is multifactorial. Thus, further studies needed to identify genetic predisposition to chronic hepatitis B infection.

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Association of tumor necrosis factor-α and -β gene polymorphisms in inflammatory bowel disease

Journal of Inflammation Research ◽

10.2147/jir.s101225 ◽

2016 ◽

pp. 133 ◽

Cited By ~ 3

Author(s):

Misbahul Arfin ◽

Ebtissam Al-Meghaiseeb ◽

Abdulrahman Al-Robayan ◽

Mulfi Al-Otaibi ◽

Abdulrahman Al-Asmari

Keyword(s):

Inflammatory Bowel Disease ◽

Tumor Necrosis Factor ◽

Tumor Necrosis Factor Alpha ◽

Gene Polymorphisms ◽

Tumor Necrosis ◽

Necrosis Factor Alpha ◽

Inflammatory Bowel ◽

Factor Alpha ◽

Beta Gene ◽

Necrosis Factor

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DETEKSI GEN IL-6 DAN TNF-α DENGAN METODE PCR PADA PENDERITA HEPATITIS B DI LABORATORIUM KLINIK MAXIMA KOTA KENDARI

BIOMA JURNAL BIOLOGI MAKASSAR ◽

10.20956/bioma.v7i1.18627 ◽

2021 ◽

Vol 7 (1) ◽

pp. 21-28

Author(s):

Sanatang Abbas ◽

Sri Anggarini Rasyid ◽

Tiara Mayang Pratiwi Lio

Keyword(s):

Tumor Necrosis Factor ◽

Hepatitis B ◽

Interleukin 6 ◽

Tumor Necrosis ◽

Virus Hepatitis ◽

Necrosis Factor Alpha ◽

Tnf Α ◽

Factor Alpha ◽

Polymerase Chain ◽

Necrosis Factor

Penyakit Hepatitis B adalah inflamasi yang terjadi pada organ hati yang dapat disebabkan oleh virus hepatitis B. Pada saat terjadi inflamasi sitokin yang ada dalam tubuh akan merespon atau mengenali jenis patogen berupa virus yang masuk ke dalam tubuh. Tumor Necrosis Factor (TNF-α) adalah salah satu sitokin pro-inflamasi yang berperan dalam proses inflamasi hati, dan Interleukin-6 (IL-6) adalah sitokin yang disekresikan dari jaringan tubuh pada fase infeksi akut atau kronik. Tujuan dari penelitian ini adalah untuk mendeteksi gen TNF-α dan IL-6 pada penderita hepatitis B dengan metode polymerase chain reaction (PCR). Jenis penelitian yang digunakan dalam penelitian ini adalah semi kuantitatif, dengan desain penelitian eksperimental. Populasi pada penelitian adalah seluruh penderita suspek yang melakukan pemeriksaan rapid Hepatitis B (HbsAg) di Laboratorium Klinik Maxima Kota Kendari sebanyak 7 orang. Teknik penarikan sampel menggunakan total sampling dengan kriteria inklusi sampel yaitu pasien yang tidak memiliki riwayat penyakit lain selain hepatitis B. Berdasarkan hasil penelitian diketahui bahwa dari ketujuh sampel penderita hepatitis B yang diperiksa menggunakan metode PCR 3 sampel dengan hasil positif (45%) terhadap gen TNF-α dan 7 (100%) hasil negative terhadap gen Interleukin 6 (IL-6). Sehingga dapat di simpulkan bahwa jenis sitokin yang berperan saat terjadi inflamasi ketika seseorang terinfeksi Virus Hepatitis B adalah Tumor Necrosis Factor Alpha (TNF-α).

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