Dried blood spots are a useful tool for hepatitis A screening in the pandemic areas with medical resource-limited condition

2020 ◽  
Vol 73 ◽  
pp. S799
Author(s):  
Chikako Yamamoto ◽  
Junko Tanaka ◽  
Vichit Ork ◽  
Shintaro Nagashima ◽  
Channarena Chuon ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Dried Blood Spots ◽  
Medical Resource ◽  
Blood Spots ◽  
Limited Condition ◽  
Resource Limited

scholarly journals HIV-1 RNA testing of pooled dried blood spots is feasible to diagnose acute HIV infection in resource limited settings

2017 ◽  
Vol 33 (2) ◽  
pp. 50-53
Author(s):  
Wentzel Dowling ◽  
Kirsten Veldsman ◽  
Mary Grace Katusiime ◽  
Jean Maritz ◽  
Peter Bock ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Dried Blood Spots ◽  
Resource Limited Settings ◽  
Acute Hiv Infection ◽  
Blood Spots ◽  
Resource Limited ◽  
Acute Hiv ◽  
Hiv 1

An outbreak of hepatitis A affecting a nursery school and a primary school

2010 ◽  
Vol 139 (3) ◽  
pp. 336-343 ◽  
Author(s):  
N. McFARLAND ◽  
M. DRYDEN ◽  
M. RAMSAY ◽  
R. S. TEDDER ◽  
S. L. NGUI
Keyword(s):  
Primary School ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Nursery School ◽  
Dried Blood Spots ◽  
Asymptomatic Infection ◽  
Maternity Unit ◽  
Subsequent Infection ◽  
Blood Spots ◽  
Direct Linkage

SUMMARYBetween March and June 2008, 12 cases of hepatitis A were notified in Winchester. Cases were from a primary school and a nursery school with no direct linkage. Hepatitis A virus (HAV) RNA sequenced from nine cases confirmed the strain in both schools to be identical. The outbreak could have affected three other schools and a maternity unit and was controlled by immunization and screening of neonates in the maternity unit by dried blood spots. No neonates were infected and no further cases were reported until 5 months later when the index case's mother became infected with same strain of virus associated with the outbreak despite vaccination. Neither the source of the outbreak or the subsequent infection of the index case's mother was identified; however, with the timing of the cases continued transmission in the community by children with asymptomatic infection or a recurrent source cannot be ruled out.

scholarly journals Use of Dried Blood Spots to Elucidate Full-Length Transmitted/Founder HIV-1 Genomes

2016 ◽  
Vol 1 (1) ◽  
pp. 129 ◽  
Author(s):  
Jesus F. Salazar-Gonzalez ◽  
Maria G. Salazar ◽  
Damien C. Tully ◽  
Colin B. Ogilvie ◽  
Gerald H. Learn ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Full Length ◽  
Cold Chain ◽  
Clinical Samples ◽  
Envelope Gene ◽  
Acid Extraction ◽  
Resource Limited Settings ◽  
Blood Spots ◽  
Resource Limited ◽  
Hiv 1

Background: Identification of HIV-1 genomes responsible for establishing clinical infection in newly infected individuals is fundamental to prevention and pathogenesis research. Processing, storage, and transportation of the clinical samples required to perform these virologic assays in resource-limited settings requires challenging venipuncture and cold chain logistics. Here, we validate the use of dried-blood spots (DBS) as a simple and convenient alternative to collecting and storing frozen plasma.Methods: We performed parallel nucleic acid extraction, single genome amplification (SGA), next generation sequencing (NGS), and phylogenetic analyses on plasma and DBS.Results: We demonstrated the capacity to extract viral RNA from DBS and perform SGA to infer the complete nucleotide sequence of the transmitted/founder (TF) HIV-1 envelope gene and full-length genome in two acutely infected individuals. Using both SGA and NGS methodologies, we showed that sequences generated from DBS and plasma display comparable phylogenetic patterns in both acute and chronic infection. SGA was successful on samples with a range of plasma viremia, including samples as low as 1,700 copies/ml and an estimated ~50 viral copies per blood spot. Further, we demonstrated reproducible efficiency in gp160 env sequencing in DBS stored at ambient temperature for up to three weeks or at -20ºC for up to five months.Conclusions: These findings support the use of DBS as a practical and cost-effective alternative to frozen plasma for clinical trials and translational research conducted in resource-limited settings.

scholarly journals Nontargeted Mass Spectrometry of Dried Blood Spots for Interrogation of the Human Circulating Metabolome

2021 ◽  
Author(s):  
Casey Ward ◽  
Shriram Nallamshetty ◽  
Jeramie D. Wartrous ◽  
Eowyn Acres ◽  
Tao Long ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Room Temperature ◽  
Dried Blood Spots ◽  
Sample Collection ◽  
Blood Spots ◽  
Resource Limited ◽  
Metabolomics Study ◽  
Simultaneous Measure ◽  
Analytical Approaches ◽  
And Storage

Advances in high resolution, nontargeted mass spectrometry allow for the simultaneous measure of thousands of metabolites in a single biosample. Application of these analytical approaches to population-scale human studies has been limited by the need for resource-intensive blood sample collection, preparation, and storage. Dried blood spotting, a technique developed decades ago for newborn screening, may offer a simple approach to overcome barriers in human blood acquisition and storage. In this study, we find that over 4,000 spectral features across diverse chemical classes may be efficiently and reproducibly extracted and relatively quantified from human dried blood spots using nontargeted mass spectrometry-based metabolomics. Moreover, over 80% of metabolites were found to be chemically stable in dried blood spots stored at room temperature for up to a week. In direct relation to plasma samples, dried blood spots exhibited comparable representation of the human circulating metabolome, capturing both known and previously uncharacterized metabolites. Dried blood spot approaches provide an opportunity for rapid and facile human biosampling and storage, and will enable widespread metabolomics study of populations, particularly in resource-limited areas.

scholarly journals Nontargeted Mass Spectrometry of Dried Blood Spots for Interrogation of the Human Circulating Metabolome

2021 ◽  
Author(s):  
Casey Ward ◽  
Shriram Nallamshetty ◽  
Jeramie D. Wartrous ◽  
Eowyn Acres ◽  
Tao Long ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Room Temperature ◽  
Dried Blood Spots ◽  
Sample Collection ◽  
Blood Spots ◽  
Resource Limited ◽  
Metabolomics Study ◽  
Simultaneous Measure ◽  
Analytical Approaches ◽  
And Storage

Advances in high resolution, nontargeted mass spectrometry allow for the simultaneous measure of thousands of metabolites in a single biosample. Application of these analytical approaches to population-scale human studies has been limited by the need for resource-intensive blood sample collection, preparation, and storage. Dried blood spotting, a technique developed decades ago for newborn screening, may offer a simple approach to overcome barriers in human blood acquisition and storage. In this study, we find that over 4,000 spectral features across diverse chemical classes may be efficiently and reproducibly extracted and relatively quantified from human dried blood spots using nontargeted mass spectrometry-based metabolomics. Moreover, over 80% of metabolites were found to be chemically stable in dried blood spots stored at room temperature for up to a week. In direct relation to plasma samples, dried blood spots exhibited comparable representation of the human circulating metabolome, capturing both known and previously uncharacterized metabolites. Dried blood spot approaches provide an opportunity for rapid and facile human biosampling and storage, and will enable widespread metabolomics study of populations, particularly in resource-limited areas.

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