How are nucleic acids released in cells from cationic lipid-nucleic acid complexes?

Cationic lipids have been extensively studied for their ability to complex with nucleic acids to condense and consequently deliver them into the cells.

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Re-Evaluating the Spherical-Nucleic-Acid Technology

10.26434/chemrxiv.6633980.v3 ◽

2018 ◽

Author(s):

Maria Czarnek ◽

David Mason ◽

Gal Haimovich ◽

Víctor F. Puntes ◽

Paolo Bergese ◽

...

Keyword(s):

Nucleic Acid ◽

Nucleic Acids ◽

Spherical Nucleic Acid ◽

Spherical Nucleic Acids ◽

In Cells

We re-evaluate the evidence presented for the detection of mRNA in cells and tissues with Spherical Nucleic Acids.<br>

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An Improved 4’-Aminomethyltroxsalen-Based DNA Crosslinker for Biotinylation of DNA

10.1101/2020.02.29.971317 ◽

2020 ◽

Author(s):

Kevin Wielenberg ◽

Miao Wang ◽

Min Yang ◽

Abdullah Ozer ◽

John T. Lis ◽

...

Keyword(s):

Nucleic Acid ◽

Nucleic Acids ◽

Uv Light ◽

Dna And Rna ◽

Double Stranded Dna ◽

Long Wave ◽

Genome Wide ◽

In Cells

AbstractNucleic acid crosslinkers that covalently join commentary strands have applications as both pharmaceuticals and biochemical probes. Psoralen is a popular crosslinker moiety that reacts with double stranded DNA and RNA upon irradiation with long wave UV light. A commercially available compound EZ-Link Psoralen-PEG3-Biotin has been used in many studies to crosslink DNA and double strand RNA for genome-wide investigations. Here we present a novel probe, AP3B, which uses a psoralen derivative, 4’-aminomethyltrioxsalen, to biotinylate nucleic acids. We show that this compound is 8-fold more effective at labeling DNA in cells and several hundred-fold more effective at crosslinking two strands of DNA in vitro than the commercially available compound EZ-Link Psoralen-PEG3-Biotin.

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Photo-Cross-Linkable Artificial Nucleic Acid: Synthesis and Properties of 3-Cyanovinylcarbazole-Modified Nucleic Acids and Its Photo-Induced Gene-Silencing Activity in Cells

Synthesis of Therapeutic Oligonucleotides ◽

10.1007/978-981-13-1912-9_10 ◽

2018 ◽

pp. 171-186

Author(s):

Takashi Sakamoto ◽

Kenzo Fujimoto

Keyword(s):

Nucleic Acid ◽

Nucleic Acids ◽

Gene Silencing ◽

Acid Synthesis ◽

Nucleic Acid Synthesis ◽

Modified Nucleic Acids ◽

In Cells

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Re-Evaluating the Spherical-Nucleic-Acid Technology

10.26434/chemrxiv.6633980.v2 ◽

2018 ◽

Author(s):

Maria Czarnek ◽

David Mason ◽

Gal Haimovich ◽

Víctor F. Puntes ◽

Paolo Bergese ◽

...

Keyword(s):

Nucleic Acid ◽

Nucleic Acids ◽

Spherical Nucleic Acid ◽

Spherical Nucleic Acids ◽

In Cells

We re-evaluate the evidence presented for the detection of mRNA in cells and tissues with Spherical Nucleic Acids.<br>

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Nucleic Acid Molecules Prepared by Monolayer Techniques

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094140 ◽

1972 ◽

Vol 30 ◽

pp. 178-179

Author(s):

Dimitrij Lang

Keyword(s):

Electron Microscopy ◽

Standard Deviation ◽

Nucleic Acid ◽

Cytochrome C ◽

Nucleic Acids ◽

Contour Length ◽

Sample Standard Deviation ◽

Molecular Weights ◽

Length Measurements ◽

Protein Monolayer

The success of the protein monolayer technique for electron microscopy of individual DNA molecules is based on the prevention of aggregation and orientation of the molecules during drying on specimen grids. DNA adsorbs first to a surface-denatured, insoluble cytochrome c monolayer which is then transferred to grids, without major distortion, by touching. Fig. 1 shows three basic procedures which, modified or not, permit the study of various important properties of nucleic acids, either in concert with other methods or exclusively:1) Molecular weights relative to DNA standards as well as number distributions of molecular weights can be obtained from contour length measurements with a sample standard deviation between 1 and 4%.

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Snapshot blotting: The transfer of nucleic acids and nucleoprotein complexes from electrophoresis gels to EM grids

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100124215 ◽

1992 ◽

Vol 50 (1) ◽

pp. 762-763

Author(s):

Stephen D. Jett

Keyword(s):

Nucleic Acid ◽

Nucleic Acids ◽

Nucleic Acid Binding ◽

Mobility Shift ◽

Carbon Coated ◽

Nucleoprotein Complexes ◽

Mobility Shift Assay ◽

Protein Nucleic Acid ◽

Gel Mobility Shift ◽

Nucleic Acid Interactions

The electrophoresis gel mobility shift assay is a popular method for the study of protein-nucleic acid interactions. The binding of proteins to DNA is characterized by a reduction in the electrophoretic mobility of the nucleic acid. Binding affinity, stoichiometry, and kinetics can be obtained from such assays; however, it is often desirable to image the various species in the gel bands using TEM. Present methods for isolation of nucleoproteins from gel bands are inefficient and often destroy the native structure of the complexes. We have developed a technique, called “snapshot blotting,” by which nucleic acids and nucleoprotein complexes in electrophoresis gels can be electrophoretically transferred directly onto carbon-coated grids for TEM imaging.

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