PNEUMOLYSIN IN URINE: A RAPID ANTIGEN DETECTION METHOD TO DIAGNOSE PNEUMOCOCCAL PNEUMONIA IN CHILDREN

In this research, we have constructed and optimized the colloidal gold labeled lateral flow strip (LFS) for rapid detection of antigen of SARS-CoV-2 and rapid screening of COVID-19. Based on the constructed and optimized colloidal gold lateral flow strip, the parameters of the LFS have been well evaluated with the clinical samples in the professional labs. The screening performance have also been evaluated from the aspects including the CT values, age distribution and onset of symptoms. Finally, based on the detection results of 420 clinical samples, the LFS can achieve the screening of COVID-19 with the positive percentage agreement (PPA, sensitivity), negative percent agreement (NPA, specificity), the positive predictive value (PPV) and the negative predictive value (NPV) of 96.8%, 100%, 100% and 96.6%, respectively, indicating the powerful potential for practical screening applications in pandemic control. Of great significance, this developed SARS-CoV-2 antigen detection method has also been successfully utilized for screening of delta-variant of SARS-CoV-2.

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Multiplex Urinary Antigen Detection for 13 Streptococcus pneumoniae Serotypes Improves Diagnosis of Pneumococcal Pneumonia in South African HIV-Infected Adults

Journal of Clinical Microbiology ◽

10.1128/jcm.01573-16 ◽

2016 ◽

Vol 55 (1) ◽

pp. 302-312 ◽

Cited By ~ 3

Author(s):

Werner C. Albrich ◽

Michael W. Pride ◽

Shabir A. Madhi ◽

Jan Callahan ◽

Peter V. Adrian ◽

...

Keyword(s):

South African ◽

Pneumococcal Pneumonia ◽

Antigen Detection ◽

Urinary Antigen ◽

Content Type ◽

Urinary Antigen Detection ◽

Dominant Serotype ◽

Quellung Reaction ◽

Urine Specimens ◽

Asymptomatic Hiv

ABSTRACT A serotype-specific urinary antigen detection (UAD) assay for 13 serotypes included in the pneumococcal conjugate vaccine (PCV13) was recently reported as a useful diagnostic tool for pneumococcal pneumonia. We aimed to assess the diagnostic accuracy of the UAD in HIV-infected South African adults. Urine specimens from a well-defined cohort of HIV-infected South African adults with pneumonia were evaluated retrospectively in the UAD assay. Pneumonia was considered pneumococcal if either sputum Gram stain, sputum culture, blood culture, or the immunochromatographic (ICT) BinaxNow S. pneumoniae test (composite diagnostic) was positive. Among 235 enrolled pneumonia patients, the UAD assay was more frequently positive (104 [44.3%]) than the composite diagnostic (71 [30.2%]; P < 0.001) and increased the pneumococcal etiology from 30.2% by an additional 22.6% to 52.8%. The UAD assay detected more pneumococcal etiologies (45.0%) than the serotype-independent ICT (23.4%, P < 0.001). UAD identified 6/7 patients with PCV13 serotype bacteremia without misclassification of bacteremia episodes due to non-PCV13 serotypes. UAD was positive for 5.1% of asymptomatic HIV-infected persons, with higher rates among those with nasopharyngeal carriage. Concordance between serotypes identified by UAD and by Quellung reaction and PCR serotyping was 70/86 (81.4%). UAD identified the dominant serotype in multiple serotype carriage. This study confirms the utility of the UAD assay for HIV-infected adults comparing favorably with other diagnostic tests. A highly valent UAD may become a new standard for detection of pneumococcal pneumonia in adults. Prior to PCV introduction, at least 53% of pneumonia cases were due to pneumococci in HIV-infected South African adults.

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Detection of Antigenemia by Enzyme-Linked Immunosorbent Assay in Horses with Experimental Ehrlichia Risticii Infection

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063879300500108 ◽

1993 ◽

Vol 5 (1) ◽

pp. 33-36 ◽

Cited By ~ 1

Author(s):

Richard E. Corstvet ◽

Stephen D. Gaunt ◽

Phillip A. Karns ◽

David Burgermeister ◽

Jere W. McBride ◽

...

Keyword(s):

Monoclonal Antibody ◽

Immunosorbent Assay ◽

Detection Method ◽

Serum Antibody ◽

Clinical Signs ◽

Clinical Findings ◽

Antigen Detection ◽

Enzyme Linked Immunosorbent Assay ◽

Infected Horse ◽

Ehrlichia Risticii

Four horses were inoculated with Ehrlichia risticii contained in either infected murine P388 D1 cells or heparinized blood from an infected horse. All 4 horses produced serum antibody, plasma antigen, and clinical signs of the disease. An enzyme-linked immunosorbent assay was used to detect antibody in the serum and was also used in conjunction with an anti- E. risticii monoclonal antibody to detect antigenemia. These laboratory and clinical findings were correlated to determine the efficiency of the antigen detection method for discerning E. risticii infection.

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Comparison of culture and antigen detection method from cerebrospinal fluid for diagnosis of acute pyogenic meningitis due to Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae type b

Journal of Patient Safety & Infection Control ◽

10.1016/j.jpsic.2015.10.114 ◽

2015 ◽

Vol 3 (2) ◽

pp. 83-84

Author(s):

N. Mehta ◽

P. Bhakare ◽

A. De ◽

A. Sonavane ◽

S. Baveja

Keyword(s):

Cerebrospinal Fluid ◽

Streptococcus Pneumoniae ◽

Haemophilus Influenzae ◽

Neisseria Meningitidis ◽

Detection Method ◽

Antigen Detection ◽

Haemophilus Influenzae Type ◽

Haemophilus Influenzae Type B ◽

Type B ◽

Pyogenic Meningitis

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Rapid diagnosis of pneumococcal pneumonia among HIV-infected adults with urine antigen detection

Journal of Infection ◽

10.1016/j.jinf.2007.06.014 ◽

2007 ◽

Vol 55 (4) ◽

pp. 300-309 ◽

Cited By ~ 50

Author(s):

David R. Boulware ◽

Charles L. Daley ◽

Cynthia Merrifield ◽

Philip C. Hopewell ◽

Edward N. Janoff

Keyword(s):

Pneumococcal Pneumonia ◽

Rapid Diagnosis ◽

Antigen Detection ◽

Urine Antigen

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cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method

BIO-PROTOCOL ◽

10.21769/bioprotoc.3457 ◽

2019 ◽

Vol 9 (24) ◽

Author(s):

Chathuni Jayathilake ◽

Takuya Terai ◽

Naoto Nemoto

Keyword(s):

Detection Method ◽

Antigen Detection

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Modern understanding about the role of group A β -hemolytic streptococcus in acute tonsillitis

Journal Infectology ◽

10.22625/2072-6732-2021-13-4-66-71 ◽

2021 ◽

Vol 13 (4) ◽

pp. 66-71

Author(s):

N. T. Mirzoev ◽

S. N. Sidorchuk ◽

Yu. I. Bulan’kov ◽

K. V. Kas’janenko

Keyword(s):

Predictive Value ◽

Bacterial Culture ◽

Detection Method ◽

Throat Swab ◽

Diagnostic System ◽

Antigen Detection ◽

Acute Tonsillitis ◽

Hemolytic Streptococcus ◽

Streptococcal Antigen ◽

Group A

Objective: assess the modern value of group А β-hemolytic streptococcus in patients with acute tonsillitis and the effectiveness of the rapid streptococcal antigen detection method.Materials and methods: microbial landscape assessment of acute tonsillitis was based on retrospective analysis of 902 bacterial culture results of a throat swab of patients with syndromes of acute tonsillitis treated in the Infectious Diseases Clinic of the Military Medical Academy named after S.M. Kirov during the period of 2019-2020. The effectiveness of the rapid streptococcal antigen detection method in the oropharynx was determined by a prospective study involving 35 patients with acute tonsillitis.Results: in the study, we have found that bacterial culture results of a throat swab, the following were more common: Nesseria species (39 %), Streptococcus viridans (23 %), and Staphylococcus aureus (17 %). The frequency of detection of β-hemolytic streptococcus was 1 %. The rapid diagnostic system «Streptatest» in patients with acute tonsillitis has demonstrated efficiency, under which that sensitivity of test was 80 %, specificity – 90 %, positive predictive value – 57,14 %, negative predictive value – 96,43 %.Conclusions: the frequency of group A β-hemolytic streptococcus in patients with lesion of lymphoid tissues of the oropharynx has declined significantly nowadays. The rapid diagnostic system «Streptatest» is a highly effective medical product that can be used in both hospital and pre-hospital stage.

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A Valuable Antigen Detection Method for Diagnosis of Acute Hepatitis E

Journal of Clinical Microbiology ◽

10.1128/jcm.01853-14 ◽

2014 ◽

Vol 53 (3) ◽

pp. 782-788 ◽

Cited By ~ 30

Author(s):

Gui-Ping Wen ◽

Zi-Min Tang ◽

Fan Yang ◽

Ke Zhang ◽

Wen-Fang Ji ◽

...

Keyword(s):

Acute Hepatitis ◽

Detection Method ◽

Public Health Problem ◽

Hepatitis E ◽

Antigen Detection ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

Detection Rates ◽

Acute Hepatitis E ◽

Capture Antibody

Hepatitis E virus (HEV) is a serious public health problem. The commonly used tests that are specific for current HEV infection diagnosis include the detection of anti-HEV IgM and HEV RNA. Here, we report an improved enzyme-linked immunosorbent assay (ELISA) method for HEV antigen detection with a linear range equivalent to 6.3 × 103to 9.2 × 105RNA copies per ml. The monoclonal antibody (MAb) 12F12, a high-ability MAb that binds HEV virus, was selected as the capture antibody from a panel of 95 MAbs. The positive period of HEV antigenemia in infected monkeys using this test was, on average, 3 weeks longer than previously reported and covered the majority of the acute phase. The positive detection rates of IgM, RNA, and new antigen from the first serum samples collected from 16 confirmed acute hepatitis E patients were 81% (13/16), 81% (13/16), and 100% (16/16), respectively. In three patients, the initial serum specimens that tested negative for IgM, despite the presence of symptoms of acute hepatitis and elevated alanine aminotransferase (ALT) levels, were positive for HEV antigen and HEV RNA. In contrast, the serum samples of the three RNA-negative patients were antigen positive (and IgM positive), possibly due to the degradation of HEV nucleic acids. Our results suggest that this new antigen detection method has acceptable concordance with RNA detection and could serve as an important tool for diagnosing acute hepatitis E.

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