scholarly journals cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method

BIO-PROTOCOL ◽  
2019 ◽  
Vol 9 (24) ◽  
Author(s):  
Chathuni Jayathilake ◽  
Takuya Terai ◽  
Naoto Nemoto
Keyword(s):  
Detection Method ◽  
Antigen Detection

scholarly journals Challenges of SERS technology as a non-nucleic acid or -antigen detection method for SARS-CoV-2 virus and its variants

2021 ◽  
Vol 181 ◽  
pp. 113153
Author(s):  
Jaya Sitjar ◽  
Jiunn-Der Liao ◽  
Han Lee ◽  
Huey-Pin Tsai ◽  
Jen-Ren Wang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Detection Method ◽  
Antigen Detection

scholarly journals Development and Performance verification of colloidal gold labeled SARS-CoV-2 antigen detection method for routine popular screening of COVID-19 with clinical samples in Poland and China

2021 ◽  
Author(s):  
Chuanxiang Guo ◽  
Li Yao ◽  
Fengling Chen ◽  
Chao Zhang ◽  
Wei Chen
Keyword(s):  
Predictive Value ◽  
Colloidal Gold ◽  
Detection Method ◽  
Age Distribution ◽  
Antigen Detection ◽  
Lateral Flow ◽  
Rapid Screening ◽  
Clinical Samples ◽  
Lateral Flow Strip ◽  
And Performance

In this research, we have constructed and optimized the colloidal gold labeled lateral flow strip (LFS) for rapid detection of antigen of SARS-CoV-2 and rapid screening of COVID-19. Based on the constructed and optimized colloidal gold lateral flow strip, the parameters of the LFS have been well evaluated with the clinical samples in the professional labs. The screening performance have also been evaluated from the aspects including the CT values, age distribution and onset of symptoms. Finally, based on the detection results of 420 clinical samples, the LFS can achieve the screening of COVID-19 with the positive percentage agreement (PPA, sensitivity), negative percent agreement (NPA, specificity), the positive predictive value (PPV) and the negative predictive value (NPV) of 96.8%, 100%, 100% and 96.6%, respectively, indicating the powerful potential for practical screening applications in pandemic control. Of great significance, this developed SARS-CoV-2 antigen detection method has also been successfully utilized for screening of delta-variant of SARS-CoV-2.

scholarly journals Detection of Antigenemia by Enzyme-Linked Immunosorbent Assay in Horses with Experimental Ehrlichia Risticii Infection

1993 ◽  
Vol 5 (1) ◽  
pp. 33-36 ◽  
Author(s):  
Richard E. Corstvet ◽  
Stephen D. Gaunt ◽  
Phillip A. Karns ◽  
David Burgermeister ◽  
Jere W. McBride ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Detection Method ◽  
Serum Antibody ◽  
Clinical Signs ◽  
Clinical Findings ◽  
Antigen Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infected Horse ◽  
Ehrlichia Risticii

Four horses were inoculated with Ehrlichia risticii contained in either infected murine P388 D1 cells or heparinized blood from an infected horse. All 4 horses produced serum antibody, plasma antigen, and clinical signs of the disease. An enzyme-linked immunosorbent assay was used to detect antibody in the serum and was also used in conjunction with an anti- E. risticii monoclonal antibody to detect antigenemia. These laboratory and clinical findings were correlated to determine the efficiency of the antigen detection method for discerning E. risticii infection.

scholarly journals Modern understanding about the role of group A β -hemolytic streptococcus in acute tonsillitis

2021 ◽  
Vol 13 (4) ◽  
pp. 66-71
Author(s):  
N. T. Mirzoev ◽  
S. N. Sidorchuk ◽  
Yu. I. Bulan’kov ◽  
K. V. Kas’janenko
Keyword(s):  
Predictive Value ◽  
Bacterial Culture ◽  
Detection Method ◽  
Throat Swab ◽  
Diagnostic System ◽  
Antigen Detection ◽  
Acute Tonsillitis ◽  
Hemolytic Streptococcus ◽  
Streptococcal Antigen ◽  
Group A

Objective: assess the modern value of group А β-hemolytic streptococcus in patients with acute tonsillitis and the effectiveness of the rapid streptococcal antigen detection method.Materials and methods: microbial landscape assessment of acute tonsillitis was based on retrospective analysis of 902 bacterial culture results of a throat swab of patients with syndromes of acute tonsillitis treated in the Infectious Diseases Clinic of the Military Medical Academy named after S.M. Kirov during the period of 2019-2020. The effectiveness of the rapid streptococcal antigen detection method in the oropharynx was determined by a prospective study involving 35 patients with acute tonsillitis.Results: in the study, we have found that bacterial culture results of a throat swab, the following were more common: Nesseria species (39 %), Streptococcus viridans (23 %), and Staphylococcus aureus (17 %). The frequency of detection of β-hemolytic streptococcus was 1 %. The rapid diagnostic system «Streptatest» in patients with acute tonsillitis has demonstrated efficiency, under which that sensitivity of test was 80 %, specificity – 90 %, positive predictive value – 57,14 %, negative predictive value – 96,43 %.Conclusions: the frequency of group A β-hemolytic streptococcus in patients with lesion of lymphoid tissues of the oropharynx has declined significantly nowadays. The rapid diagnostic system «Streptatest» is a highly effective medical product that can be used in both hospital and pre-hospital stage. 

scholarly journals A Valuable Antigen Detection Method for Diagnosis of Acute Hepatitis E

2014 ◽  
Vol 53 (3) ◽  
pp. 782-788 ◽  
Author(s):  
Gui-Ping Wen ◽  
Zi-Min Tang ◽  
Fan Yang ◽  
Ke Zhang ◽  
Wen-Fang Ji ◽  
...  
Keyword(s):  
Acute Hepatitis ◽  
Detection Method ◽  
Public Health Problem ◽  
Hepatitis E ◽  
Antigen Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Detection Rates ◽  
Acute Hepatitis E ◽  
Capture Antibody

Hepatitis E virus (HEV) is a serious public health problem. The commonly used tests that are specific for current HEV infection diagnosis include the detection of anti-HEV IgM and HEV RNA. Here, we report an improved enzyme-linked immunosorbent assay (ELISA) method for HEV antigen detection with a linear range equivalent to 6.3 × 103to 9.2 × 105RNA copies per ml. The monoclonal antibody (MAb) 12F12, a high-ability MAb that binds HEV virus, was selected as the capture antibody from a panel of 95 MAbs. The positive period of HEV antigenemia in infected monkeys using this test was, on average, 3 weeks longer than previously reported and covered the majority of the acute phase. The positive detection rates of IgM, RNA, and new antigen from the first serum samples collected from 16 confirmed acute hepatitis E patients were 81% (13/16), 81% (13/16), and 100% (16/16), respectively. In three patients, the initial serum specimens that tested negative for IgM, despite the presence of symptoms of acute hepatitis and elevated alanine aminotransferase (ALT) levels, were positive for HEV antigen and HEV RNA. In contrast, the serum samples of the three RNA-negative patients were antigen positive (and IgM positive), possibly due to the degradation of HEV nucleic acids. Our results suggest that this new antigen detection method has acceptable concordance with RNA detection and could serve as an important tool for diagnosing acute hepatitis E.

scholarly journals Can a ‘3-tier Screening’ Strategy be Adopted during COVID-19 Pandemic for Safer Air Travel and to Help Curb its Spread Across Borders?

2021 ◽  
Vol 1 (1) ◽  
pp. 62-64
Author(s):  
Mohammad Shahid
Keyword(s):  
Detection Method ◽  
Screening Strategy ◽  
Air Travel ◽  
Antigen Detection ◽  
Destination Country ◽  
Rt Pcr ◽  
Screening Protocol ◽  
Entire World ◽  
Pcr Test

Since the appearance of SARS-CoV-2 in 2019, it spread quickly crossing geographical borders and thus affected almost the entire world. It was alarming to note its quick spread, which obviously was due to the increased frequency and ease of air travel in this era. Currently, many airlines (and countries too) have a prerequisite to have a negative COVID-19 RT-PCR test within 72 hrs. prior to boarding the flight. Although all the necessary precautions are strictly enforced during air travel, there is still a possibility that a person with a negative COVID-19 test (RT-PCR) around 72hrs prior to boarding the flight would have an infection and that the person would pass it on to fellow passengers on board and thus can further spread SARS-CoV-2 infection into the community if robust action is not initiated. There is also a subconscious apprehension among the passengers that co-passengers may have an infection on board. This is especially worrisome seeing the appearance of new variants recently. Here I present the logistics for a ‘3-tier screening’ protocol (1st test by RT-PCR within 72hrs of the flight schedule, 2nd test by rapid antigen detection method 1-5hrs prior to flight schedule, 3rd test post-arrival or to follow the destination country post-arrival protocol), which would at least provide an extra filter to separate the recently identified positive cases and thus prevent the spread of this threatening disease across the borders.

Comparison of a nested polymerase chain reaction – restriction fragment length polymorphism method, the PATH antigen detection method, and microscopy for the detection and identification of malaria parasites

2001 ◽  
Vol 47 (10) ◽  
pp. 903-907
Author(s):  
Richard Parkes ◽  
Teresa Lo ◽  
Quantine Wong ◽  
Judith L Isaac-Renton ◽  
Sean K Byrne
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Fragment Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Detection Method ◽  
Antigen Detection ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Restriction Fragment Length

A nested polymerase chain reaction – restriction fragment length polymorphism (PCR–RFLP) method, the PATH antigen detection method, and light microscopy were compared for their capacity to detect and identify Plasmodium species. One hundred and thirty-six blood specimens obtained from patients suspected of having malaria were examined by each of the three methods. Forty-four specimens were positive for malaria using microscopy as the "gold standard". The sensitivity for nested PCR was 100%, and the specificity was 98%. For the detection of Plasmodium falciparum, the antigen detection method had a sensitivity of 100% and a specificity of 97%. Species identification obtained using PCR–RFLP was identical or superior to light microscopy in 42 cases (96%). Although the nested PCR–RFLP method was more sensitive and specific, the rapid turnaround time and high sensitivity of the antigen detection method makes it a useful adjunct to standard microscopy.Key words: malaria, PCR–RFLP, antigen detection.

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