Photothermal treatment of ESBL E. coli via iron oxide nanoparticles

2021 ◽  
Vol 79 ◽  
pp. S229
Author(s):  
T-W. Chang ◽  
Y-C. Chiu ◽  
C-C. Huang
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
E Coli ◽  
Photothermal Treatment

scholarly journals Effects of Change PH on The Structural and Optical Properties of Iron Oxide Nanoparticles

2021 ◽  
Vol 32 (3) ◽  
pp. 58
Author(s):  
Raad S. Sabry ◽  
Muslim A. Abid ◽  
Sarah Q. Hussein
Keyword(s):  
Particle Size ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Sem Images ◽  
Stabilizing Agents ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Different Shapes ◽  
Inhibition Zones

Iron oxide nanoparticles were made using celery extract by chemical method with change PH. Bio-materials in celery extract synthesized the iron oxide nanoparticles by reducing iron (III) chloride (FeCl3) and then acted as both capping and stabilizing agents. The iron oxide NPs were characterized by XRD, SEM, and UV–vis techniques. The change PH affected the size, shape, and purity of iron oxide NPs. XRD results showed Crystallite size increased from 16.71nm to 21.65nm as pH was increased from 1.6 to 12. SEM images showed that the particle size of (α-Fe2O3) NPs was around 40.06 nm, while increasing PH showed different shapes in the same sample.  The particle size became approximately 45.56 and 61.22 nm. UV–vis measurements showed the energy band increased from 3.11eV to 5.11eV. The antimicrobial activity of iron oxide NPs was determined by growth inhibition zones of the negative gram bacteria E. coli, Klebsiella spp, and gram-positive bacteria S. aureus, S. epidermidis, and fungal Candida albicans. The zones for (α-Fe2O3) NPs when PH 1.6 was between (12-13) mm. The zones for (α-Fe2O3) NPs when PH 12 was a little higher between (13-15) mm.

Antibacterial Effect of Silver and Iron Oxide Nanoparticles in Combination with Antibiotics on E. coli K12

2019 ◽  
Vol 9 (3) ◽  
pp. 587-596
Author(s):  
Khachatryan Anush ◽  
Kazaryan Shushanik ◽  
Tiratsuyan Susanna ◽  
Hovhannisyan Ashkhen
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Antibacterial Effect ◽  
Oxide Nanoparticles ◽  
E Coli

scholarly journals In-Vitro and In-Vivo Tolerance and Therapeutic Investigations of Phyto-Fabricated Iron Oxide Nanoparticles against Selected Pathogens

Toxics ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 105
Author(s):  
Amreen Shah ◽  
Isfahan Tauseef ◽  
Manel Ben Ali ◽  
Muhammad Arfat Yameen ◽  
Amine Mezni ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Severe Infection ◽  
Oxide Nanoparticles ◽  
Mice Model ◽  
Desorption Process ◽  
X Ray ◽  
E Coli

The Paeonia emodi (P. emodi)-mediated iron oxide nanoparticles (Fe2O3 NPs) were screened for in-vitro and in-vivo antibacterial activity against the Staphylococcus aureus (S. aureus) (ATCC #: 6538) and Escherichia coli (E. coli) (ATCC #:15224). The synthesized Fe2O3 NPs were characterized via nitrogen adsorption-desorption process, X-ray diffractometer (XRD), transmission and scanning electron microscopies (TEM and SEM), energy dispersive X-ray (EDX) and Fourier transform infrared (FTIR) spectroscopies. The SBET was found to be 94.65 m2/g with pore size of 2.99 nm, whereas the average crystallite and particles size are 23 and 27.64 nm, respectively. The 4 μg/mL is the MIC that inhibits the growth of E. coli, whereas those for S. aureus are below the detection limit (<1.76 μg/mL). The tolerance limit of the mice model was inspected by injecting different concentration of Fe2O3 NPs and bacteria suspensions. The 14 ppm suspension was the tolerated dose and the concentration above were proved lethal. The most severe infection was induced in mice with injection of 3 × 107 CFUs of both bacteria, while the inoculation of higher concentrations of bacterial suspensions resulted in the mice’s death. The histopathological and hematological studies reveals that the no/negligible infection was found in the mice exposed to the simultaneous inoculation of Fe2O3 NPs (14 ppm) and bacterial suspensions (3 × 107 CFUs).

scholarly journals Evaluation of Antibacterial Activity of Iron Oxide Nanoparticles Against Escherichia coli

2017 ◽  
Vol 2 (4) ◽  
pp. 166-169
Author(s):  
Mehrdad Khatami ◽  
Mohammad Reza Aflatoonian ◽  
Hakim Azizi ◽  
Farideh Mosazade ◽  
Ahmad Hooshmand ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Metal Oxide Nanoparticles ◽  
Inhibitory Effect ◽  
Oxide Nanoparticles ◽  
Bacterial Cells ◽  
Inexpensive Method ◽  
E Coli

Introduction: Considering the usefulness of metal oxide nanoparticles in biology and biomedicine, iron oxide nanoparticles were biosynthesized using bioresource engineering to evaluate its antibacterial activity against Escherichia coli. Methods: Macrodilution method was used for calculating the lowest concentration which prevented the growth of bacteria (minimum inhibitory concentration [MIC]), and the lowest concentration that destroyed all bacterial cells (minimum bactericidal concentration [MBC]). Results: The lowest concentration of iron oxide nanoparticles that inhibited the growth of E. coli (MIC) was recorded at 250 µg/mL. On the other hand, the MBC of iron oxide nanoparticles was calculated at 500 µg/mL. Conclusion: Iron oxide nanoparticles were produced by a green and eco-friendly, simple and inexpensive method. The results showed the inhibitory effect of iron oxide nanoparticles on E. coli at 250 µg/mL. This may suggest using these nanoparticles as potential antibacterial agents.

scholarly journals Iron Oxide Nanoparticles – Characterization and Antimicrobial Studies

2021 ◽  
Vol 6 (03) ◽  
pp. 27-32
Author(s):  
Disholin Dennison Priya ◽  
Thangavel Pichaiappa Rajesh ◽  
Rachel Syam Sundar ◽  
Chandrasekhar Narendhar
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Iron Nanoparticles ◽  
Flavonoid Glycoside ◽  
Hydroxyl Group ◽  
Oxide Nanoparticles ◽  
E Coli ◽  
Antimicrobial Studies ◽  
Uv Visible ◽  
Nanoparticles Characterization

Nanotechnology is one of the most promising technologies that give us better outcomes from biological and industrial issues. This work ismainly based on the green synthesis of iron oxide nanoparticles, assisted by the flower extract. The nanoparticles were synthesized and characterized using UV Visible spectroscopy with characteristic absorbance peaks at 300 nm and 310 nm. Prominent Fourier-Transform Infrared Spectroscopy (FTIR) peaks were obtained corresponding to phenols, amide group, aromatic ring, hydroxyl group, and carbonate ions involved in the stabilization of iron oxide nanoparticles formation. Dynamic light scattering analysis of nanoparticles showed the average sizes as 80.7 nm. Scanning electron microscope images revealed that the size of iron nanoparticles in the range of 160-300 nm. The green synthesized iron nanoparticles have promising potential to inhibit the growth of bacteria. Iron oxide nanoparticles inhibit E. coli, B. subtilis, P. aeruginosa was also enumerated as antimicrobial study. The phytochemicals alkaloid, flavonoid, glycoside, terpenoid, and saponin present in theSenna auriculata may be attributed to reducing iron oxidenanoparticles.

scholarly journals Adherence of amino acids functionalized iron oxide nanoparticles on bacterial models E. Coli and B. subtilis

2018 ◽  
Vol 987 ◽  
pp. 012044 ◽  
Author(s):  
W. Trujillo ◽  
J. Zarria ◽  
J. Pino ◽  
L. Menacho ◽  
M. Coca ◽  
...  
Keyword(s):  
Amino Acids ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
E Coli

TARGETING CYCLIN B1 WITH ANTISENSE OLIGONUCLETOTIDES- COATED SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Hosam Zaghloul ◽  
Doaa A. Shahin ◽  
Ibrahim El- Dosoky ◽  
Mahmoud E. El-awady ◽  
Fardous F. El-Senduny ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cellular Uptake ◽  
Superparamagnetic Iron Oxide ◽  
Cyclin B1 ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Mcf7 Cells ◽  
M Phase ◽  
The Impact

Antisense oligonucleotides (ASO) represent an attractive trend as specific targeting molecules but sustain poor cellular uptake meanwhile superparamagnetic iron oxide nanoparticles (SPIONs) offer stability of ASO and improved cellular uptake. In the present work we aimed to functionalize SPIONs with ASO targeting the mRNA of Cyclin B1 which represents a potential cancer target and to explore its anticancer activity. For that purpose, four different SPIONs-ASO conjugates, S-M (1–4), were designated depending on the sequence of ASO and constructed by crosslinking carboxylated SPIONs to amino labeled ASO. The impact of S-M (1–4) on the level of Cyclin B1, cell cycle, ROS and viability of the cells were assessed by flowcytometry. The results showed that S-M3 and S-M4 reduced the level of Cyclin B1 by 35 and 36%, respectively. As a consequence to downregulation of Cyclin B1, MCF7 cells were shown to be arrested at G2/M phase (60.7%). S-M (1–4) led to the induction of ROS formation in comparison to the untreated control cells. Furthermore, S-M (1–4) resulted in an increase in dead cells compared to the untreated cells and SPIONs-treated cells. In conclusion, targeting Cyclin B1 with ASO-coated SPIONs may represent a specific biocompatible anticancer strategy.

Sign in / Sign up

Export Citation Format

Share Document