miRNA-expression in prostate cancer-associated fibroblasts and their extracellular vesicles

Extracellular vesicles (EVs) are membranous nanoparticles naturally released from living cells which can be found in all types of body fluids. Recent studies found that cancer cells secreted EVs containing the unique set of biomolecules, which give rise to a distinctive absorbance spectrum representing its cancer type. In this study, we aimed to detect the medium EVs (200–300 nm) from the urine of prostate cancer patients using Fourier transform infrared (FTIR) spectroscopy and determine their association with cancer progression. EVs extracted from 53 urine samples from patients suspected of prostate cancer were analyzed and their FTIR spectra were preprocessed for analysis. Characterization of morphology, particle size and marker proteins confirmed that EVs were successfully isolated from urine samples. Principal component analysis (PCA) of the EV’s spectra showed the model could discriminate prostate cancer with a sensitivity of 59% and a specificity of 81%. The area under curve (AUC) of FTIR PCA model for prostate cancer detection in the cases with 4–20 ng/mL PSA was 0.7, while the AUC for PSA alone was 0.437, suggesting the analysis of urinary EVs described in this study may offer a novel strategy for the development of a noninvasive additional test for prostate cancer screening.

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Differential miRNA expression in pleural effusions derived from extracellular vesicles of patients with lung cancer, pulmonary tuberculosis, or pneumonia

Tumor Biology ◽

10.1007/s13277-016-5410-6 ◽

2016 ◽

Vol 37 (12) ◽

pp. 15835-15845 ◽

Cited By ~ 24

Author(s):

Jin Lin ◽

Yan Wang ◽

Ye-Qing Zou ◽

Xin Chen ◽

Bo Huang ◽

...

Keyword(s):

Lung Cancer ◽

Pulmonary Tuberculosis ◽

Extracellular Vesicles ◽

Mirna Expression ◽

Pleural Effusions

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Metastatic state of parent cells influences the uptake and functionality of prostate cancer cell-derived extracellular vesicles

Journal of Extracellular Vesicles ◽

10.1080/20013078.2017.1354645 ◽

2017 ◽

Vol 6 (1) ◽

pp. 1354645 ◽

Cited By ~ 11

Author(s):

Elisa Lázaro-Ibáñez ◽

Maarit Neuvonen ◽

Maarit Takatalo ◽

Uma Thanigai Arasu ◽

Cristian Capasso ◽

...

Keyword(s):

Prostate Cancer ◽

Cancer Cell ◽

Extracellular Vesicles ◽

Prostate Cancer Cell

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Label-Free Prostate Cancer Detection by Characterization of Extracellular Vesicles Using Raman Spectroscopy

Analytical Chemistry ◽

10.1021/acs.analchem.8b01831 ◽

2018 ◽

Vol 90 (19) ◽

pp. 11290-11296 ◽

Cited By ~ 28

Author(s):

Wooje Lee ◽

Afroditi Nanou ◽

Linda Rikkert ◽

Frank A. W. Coumans ◽

Cees Otto ◽

...

Keyword(s):

Prostate Cancer ◽

Raman Spectroscopy ◽

Extracellular Vesicles ◽

Cancer Detection ◽

Label Free ◽

Prostate Cancer Detection

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The Expression of miRNAs in Human Ovaries, Oocytes, Extracellular Vesicles, and Early Embryos: A Systematic Review

Cells ◽

10.3390/cells8121564 ◽

2019 ◽

Vol 8 (12) ◽

pp. 1564 ◽

Cited By ~ 7

Author(s):

Albert Salas-Huetos ◽

Emma R. James ◽

Kenneth I. Aston ◽

Timothy G. Jenkins ◽

Douglas T. Carrell ◽

...

Keyword(s):

Systematic Review ◽

Extracellular Vesicles ◽

Mirna Expression ◽

Observational Studies ◽

Reproductive Function ◽

Human Reproduction ◽

Reproductive Tissues ◽

Early Embryos ◽

Human Reproductive

The recent discovery of microRNAs (miRNAs) in human reproductive tissues and cells indicates a possible functional role in reproductive function. However, the studies published to date in female reproductive tissues/cells and embryos are inconclusive and sometimes controversial. In order to update the knowledge of this field, the present study aimed to discuss, through a systematic review, the role of miRNAs in female human reproduction and early embryogenesis. We conducted a systematic review of the published literature in MEDLINE and EMBASE databases through June 2018 (plus a complementary search until July 2019), in accordance with the PRISMA guidelines. We have included descriptive and observational studies, in which fertile/infertile women were well-defined. The primary outcome was the miRNA expression in ovaries, oocytes, extracellular vesicles, and embryos. We identified 25,204 articles, of which 28 were selected for qualitative analysis: 18 in ovaries and extracellular vesicles, three in oocytes, and seven in embryos. The present systematic review of descriptive and observational studies demonstrates that aberrant miRNA expression in female reproductive tissues/cells and embryos is related with infertility and embryogenesis errors. The expression of specific miRNAs, particularly in extracellular vesicles, may be used in the future as biomarkers of infertility and prognostic tools of embryo development.

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Loss of exosomal miR-146a-5p from cancer-associated fibroblasts after androgen deprivation therapy contributes to prostate cancer metastasis

Journal of Experimental & Clinical Cancer Research ◽

10.1186/s13046-020-01761-1 ◽

2020 ◽

Vol 39 (1) ◽

Author(s):

Yu Zhang ◽

Jing Zhao ◽

Mao Ding ◽

Yiming Su ◽

Di Cui ◽

...

Keyword(s):

Prostate Cancer ◽

Androgen Deprivation Therapy ◽

Cancer Metastasis ◽

Androgen Deprivation ◽

Luciferase Reporter ◽

Migration And Invasion ◽

Cancer Microenvironment ◽

Erk Pathway ◽

Cancer Associated Fibroblasts ◽

Mesenchymal Transition

Abstract Background Androgen deprivation therapy (ADT) is the backbone of therapy for advanced prostate cancer (PCa). Despite the good initial response, castration resistance and metastatic progression will inevitably occur. Cancer-associated fibroblasts (CAFs) may be implicated in promoting metastasis of PCa after ADT. Our aim is to investigate the role and mechanism of CAFs-derived exosomes involving in metastasis of PCa after ADT. Methods PCa cells were co-cultured with exosomes derived from 10 nM dihydrotestosterone (DHT)-treated (simulating the high androgen level of prostate cancer microenvironment) or ethanol (ETOH) -treated (simulating the castration level of prostate cancer microenvironment after ADT) CAFs, and their migration and invasion differences under castration condition were examined both in vitro and in vivo. The miRNA profiles of exosomes derived from DHT-treated CAFs and matched ETOH-treated CAFs were analysed via next generation sequencing. The transfer of exosomal miR-146a-5p from CAFs to PCa cells was identified by fluorescent microscopy. The function and direct target gene of exosomal miR-146a-5p in PCa cells were confirmed through Transwell assays, luciferase reporter, and western blot. Results Compared with DHT-treated CAFs, exosomes derived from ETOH-treated CAFs dramatically increased migration and invasion of PCa cells under castration condition. MiR-146a-5p level in exosomes from ETOH-treated CAFs was significantly reduced. The loss of miR-146a-5p may strengthen the epithelial-mesenchymal transition (EMT) to accelerate cancer cells metastasis by modulating epidermal growth factor receptor (EGFR)/ERK pathway. Conclusions CAFs-derived exosomal miR-146a-5p confers metastasis in PCa cells under ADT through the EGFR/ERK pathway and it may present a new treatment for PCa.

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