Effect of lipopolysaccharide on the gene expression of the enzymes involved in tetrahydrobiopterin de novo biosynthesis in murine neuroblastoma cell line N1E-115

1997 ◽  
Vol 238 (1-2) ◽  
pp. 21-24 ◽  
Author(s):  
Keiji Mori ◽  
Akira Nakashima ◽  
Toshiharu Nagatsu ◽  
Akira Ota
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
De Novo ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line ◽  
De Novo Biosynthesis ◽  
Murine Neuroblastoma

Isolation and structural analysis of a 1.2-megabase N-myc amplicon from a human neuroblastoma

1992 ◽  
Vol 12 (12) ◽  
pp. 5563-5570
Author(s):  
S S Schneider ◽  
J L Hiemstra ◽  
B A Zehnbauer ◽  
P Taillon-Miller ◽  
D L Le Paslier ◽  
...  
Keyword(s):  
Cell Line ◽  
Gene Amplification ◽  
De Novo ◽  
Physical Map ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line ◽  
Chromosome 2 ◽  
Human Neuroblastoma ◽  
Artificial Chromosomes ◽  
Yeast Artificial Chromosomes

Oncogene amplification is observed frequently in human cancers, but little is known about the mechanism of gene amplification or the structure of amplified DNA in tumor cells. We have studied the N-myc amplified domain from a representative neuroblastoma cell line, SMS-KAN, and compared the map of the amplicon in this cell line with that seen in normal DNA. The SMS-KAN cell line DNA was cloned into yeast artificial chromosomes (YACs), and clones were identified by screening the YAC library with amplified DNA probes that were obtained previously (B. Zehnbauer, D. Small, G. M. Brodeur, R. Seeger, and B. Vogelstein, Mol. Cell. Biol. 8:522-530, 1988). In addition, YAC clones corresponding to the normal N-myc locus on chromosome 2 were obtained by screening two normal human YAC libraries with these probes, and the restriction maps of the two sets of overlapping YACs were compared. Our results suggest that the amplified domain in this cell line is a approximately 1.2-Mb circular molecule with a head-to-tail configuration, and the physical map of the normal N-myc locus generally is conserved in the amplicon. These results provide a physical map of the amplified domain of a neuroblastoma cell line that has de novo amplification of an oncogene. The head-to-tail organization, the general conservation of the normal physical map in the amplicon, and the extrachromosomal location of the amplified DNA are most consistent with the episome formation-plus-segregation mechanism of gene amplification in these tumors.

Tetrahydrobiopterin Biosynthesis Enhanced by Lipopolysaccharide Stimulation in Murine Neuroblastoma Cell Line N1E-115

2002 ◽  
Vol 67 (6) ◽  
pp. 2540-2548 ◽  
Author(s):  
Akira Ota ◽  
Satoshi Yoshida ◽  
Takahide Nomura ◽  
Shigeru Matsui ◽  
Yasumichi Hagino ◽  
...  
Keyword(s):  
Cell Line ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line ◽  
Murine Neuroblastoma

scholarly journals HLXB9 Gene Expression, and Nuclear Location during In Vitro Neuronal Differentiation in the SK-N-BE Neuroblastoma Cell Line

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e105481 ◽  
Author(s):  
Claudia Giovanna Leotta ◽  
Concetta Federico ◽  
Maria Violetta Brundo ◽  
Sabrina Tosi ◽  
Salvatore Saccone
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Neuronal Differentiation ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line ◽  
Nuclear Location

scholarly journals Viologen-phosphorus dendrimers exhibit minor toxicity against a murine neuroblastoma cell line

2013 ◽  
Vol 18 (3) ◽  
Author(s):  
Joanna Lazniewska ◽  
Katarzyna Milowska ◽  
Nadia Katir ◽  
Abdelkim Kadib ◽  
Maria Bryszewska ◽  
...  
Keyword(s):  
Cell Line ◽  
Cellular Response ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cells ◽  
Neuroblastoma Cell Line ◽  
Water Soluble ◽  
Mitochondrial Activity ◽  
Murine Neuroblastoma ◽  
Biomedical Investigation ◽  
The Impact

AbstractDendrimers containing viologen (derivatives of 4,4′-bipyridyl) units in their structure have been demonstrated to exhibit antiviral activity against human immunodeficiency virus (HIV-1). It has also recently been revealed that novel dendrimers with both viologen units and phosphorus groups in their structure show different antimicrobial, cytotoxic and hemotoxic properties, and have the ability to influence the activity of cholinesterases and to inhibit α-synuclein fibrillation. Since the influence of viologen-phosphorus structures on basic cellular processes had not been investigated, we examined the impact of such macromolecules on the murine neuroblastoma cell line (N2a). We selected three water-soluble viologen-phosphorus (VPD) dendrimers, which differ in their core structure, number of viologen units and number and type of surface groups, and analyzed several aspects of the cellular response. These included cell viability, generation of reactive oxygen species (ROS), alterations in mitochondrial activity, morphological modifications, and the induction of apoptosis and necrosis. The MTT assay results suggest that all of the tested dendrimers are only slightly cytotoxic. Although some changes in ROS formation and mitochondrial function were detected, the three compounds did not induce apoptosis or necrosis. In light of these results, we can assume that the tested VPD are relatively safe for mouse neuroblastoma cells. Although more research on their safety is needed, VPD seem to be promising nanoparticles for further biomedical investigation.

Agonist selective modulation of tyrosine hydroxylase expression by cannabinoid ligands in a murine neuroblastoma cell line

2007 ◽  
Vol 102 (6) ◽  
pp. 1996-2007 ◽  
Author(s):  
Barbara Bosier ◽  
Sébastien Tilleux ◽  
Mustapha Najimi ◽  
Didier M. Lambert ◽  
Emmanuel Hermans
Keyword(s):  
Tyrosine Hydroxylase ◽  
Cell Line ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line ◽  
Murine Neuroblastoma ◽  
Selective Modulation
Sign in / Sign up

Export Citation Format

Share Document