Hydrogen-peroxide-induced heme degradation in red blood cells: the protective roles of catalase and glutathione peroxidase

2003 ◽  
Vol 1620 (1-3) ◽  
pp. 211-217 ◽  
Author(s):  
Enika Nagababu ◽  
Francis J. Chrest ◽  
Joseph M. Rifkind
Keyword(s):  
Hydrogen Peroxide ◽  
Glutathione Peroxidase ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Heme Degradation

scholarly journals An LC–MS Assay to Measure Superoxide Radicals and Hydrogen Peroxide in the Blood System

Metabolites ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 175 ◽  
Author(s):  
Ioannis Tsamesidis ◽  
Chinedu O. Egwu ◽  
Pierre Pério ◽  
Jean-Michel Augereau ◽  
Françoise Benoit-Vical ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Hydrogen Peroxide ◽  
Red Blood Cells ◽  
Blood Cells ◽  
High Resolution Mass Spectrometry ◽  
Modern Medicine ◽  
Reactive Species ◽  
Blood System ◽  
Pathological Conditions ◽  
Depth Study

Red blood cells are constantly exposed to reactive species under physiological or pathological conditions or during administration of xenobiotics. Regardless of the source, its accurate quantification is paramount in the area of theragnostics, which had been elusive up until now. Even if there are a lot of approaches to evaluate the oxidative stress, very sensitive methods are missing for the blood system. We therefore sought to apply a highly sensitive approach, by liquid chromatography coupled to mass spectrometry (UPLC–MS), for the quantification of reactive species such as superoxide radical and hydrogen peroxide using dihydroethidium (DHE) and coumarin boronic acid (CBA) probes respectively through the detection of 2-hydroxyethidium (2OH-E+) and 7-hydroxycoumarin (COH). The use of the high-resolution mass spectrometry associated to UPLC ensured a selective detection of superoxide and hydrogen peroxide in the blood system under diverse conditions such as oxidized red blood cells (RBCs), untreated and treated parasitized RBCs. Moreover, this technique allowed the determination of reactive species in human plasma. This protocol provides a huge opportunity for in-depth study of several pathological conditions vis-a-vis their treatment in modern medicine.

Hydrogen peroxide production by red blood cells

1994 ◽  
Vol 16 (1) ◽  
pp. 123-129 ◽  
Author(s):  
Cecilia Giulivi ◽  
Paul Hochstein ◽  
Kelvin J.A. Davies
Keyword(s):  
Hydrogen Peroxide ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Hydrogen Peroxide Production

scholarly journals Hydroxyurea-Induced Expression of Glutathione Peroxidase 1 in Red Blood Cells of Individuals with Sickle Cell Anemia

2010 ◽  
Vol 13 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Chun-Seok Cho ◽  
Gregory J. Kato ◽  
Seung Ha Yang ◽  
Sung Won Bae ◽  
Jong Seo Lee ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Blood Cells ◽  
Induced Expression ◽  
Glutathione Peroxidase 1
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