P01.103 Activity assessments of superoxide dismutase, glutathione reductase and glutathione peroxidase in the red blood cells of Alzheimer patient's

We report the effect of cross-sex hormonal replacement on antioxidant enzymes from rat retroperitoneal fat adipocytes. Eight rats of each gender were assigned to each of the following groups: control groups were intact female or male (F and M, resp.). Experimental groups were ovariectomized F (OvxF), castrated M (CasM), OvxF plus testosterone (OvxF + T), and CasM plus estradiol (CasM + E2) groups. After sacrifice, retroperitoneal fat was dissected and processed for histology. Adipocytes were isolated and the following enzymatic activities were determined: Cu-Zn superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GR). Also, glutathione (GSH) and lipid peroxidation (LPO) were measured. In OvxF, retroperitoneal fat increased and adipocytes were enlarged, while in CasM rats a decrease in retroperitoneal fat and small adipocytes are observed. The cross-sex hormonal replacement in F rats was associated with larger adipocytes and a further decreased activity of Cu-Zn SOD, CAT, GPx, GST, GR, and GSH, in addition to an increase in LPO. CasM + E2exhibited the opposite effects showing further activation antioxidant enzymes and decreases in LPO. In conclusion, E2deficiency favors an increase in retroperitoneal fat and large adipocytes. Cross-sex hormonal replacement in F rats aggravates the condition by inhibiting antioxidant enzymes.

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Heritability of Some Important Parameters of the Antioxidant Defense System Like Glucose-6-Phosphate Dehydrogenase, Catalase, Glutathione Peroxidase and Lipid Peroxidation in Red Blood Cells by Twin Study

International Journal of Human Genetics ◽

10.1080/09723757.2001.11885764 ◽

2001 ◽

Vol 1 (3) ◽

pp. 1-4 ◽

Cited By ~ 2

Author(s):

Shila Chakraborty ◽

A.B. Das Chaudhuri

Keyword(s):

Lipid Peroxidation ◽

Glutathione Peroxidase ◽

Red Blood Cells ◽

Twin Study ◽

Blood Cells ◽

Antioxidant Defense ◽

Antioxidant Defense System ◽

Defense System ◽

Glucose 6 Phosphate Dehydrogenase

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Comparison of oxidant and antioxidant status of seminal plasma and spermatozoa of several fish species

Czech Journal of Animal Science ◽

10.17221/6861-cjas ◽

2013 ◽

Vol 58 (No. 7) ◽

pp. 313-320 ◽

Cited By ~ 21

Author(s):

A. Shaliutina-Kolešová ◽

I. Gazo ◽

J. Cosson ◽

O. Linhart

Keyword(s):

Superoxide Dismutase ◽

Glutathione Peroxidase ◽

Glutathione Reductase ◽

Seminal Plasma ◽

Brook Trout ◽

Siberian Sturgeon ◽

Russian Sturgeon ◽

Thiobarbituric Acid Reactive Substance ◽

Acipenser Baerii ◽

Carbonyl Protein

Oxidant and antioxidant activity in seminal plasma was compared with that in spermatozoa of teleost (common carp Cyprinus carpio and brook trout Salvelinus fontinalis) and chondrostean (Russian sturgeon Acipenser gueldenstaedtii, Siberian sturgeon Acipenser baerii, and sterlet Acipenser ruthenus) fishes. No differences were found between seminal plasma and spermatozoa in the level of thiobarbituric-acid-reactive substance (0.24 ± 0.08 to 0.33 ± 0.04 nmol/mg proteins) in Russian sturgeon, Siberian sturgeon, and sterlet. Carbonyl protein concentration was significantly higher in spermatozoa than in seminal plasma of all studied species. Analyzed antioxidants included superoxide dismutase, glutathione reductase, and glutathione peroxidase activity. Significant differences (P < 0.05) were detected between seminal plasma and spermatozoa in total superoxide dismutase (SOD) and glutathione reductase (GR). Total glutathione peroxidase (GPx) activity was significantly higher in brook trout (12.56 ± 3.23 mU/mg proteins) and Russian sturgeon (11.56 ± 3.12 mU/mg proteins) spermatozoa compared to seminal plasma (6.81 ± 1.56 mU/mg proteins in brook trout and 9.56 ± 3.12 mU/mg proteins in Russian sturgeon). This study provides new data on oxidant and antioxidant balance between spermatozoa and seminal plasma that may be of value in the development of methods for artificial reproduction of teleost and chondrostean species.  

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