A complex preparations «Kahadin + OV», «Kaplaestrol + OV», «Karafest + OV» and «Karafand + OV», which have antioxidant, anti-hypoxic and membranoprotective properties thanks to nanobiomaterials.Were tested drug «Karafest + OV Zn», at doses of justification which came out of the daily needs of animals in the components of the drug, which for sheep and goats in the last 7-8 weeks of pregnancy (per animal), carotene – 0.28–0.5 mg/kg, or 12–26 mg, phytoestrogens – 0,0175 mg/kg or 0.61-1.22 mg, zinc – 1-1.08 mg/kg, or 54-70 mg.The content of substances in 1 ml «Karafest + OV Zn» according to the technical specifications are: carotene - 10 mg (1,2-2,6 ml), phytoestrogens - 1 mg (0.6-1.2 ml), zinc carbonate – 2 mg, ortovanadatе europium activated gadolinium – 0.00015 mg.The final dosage was determined by the scheme – 0.35–2.1 ml/animal оr 0.01–0.03 ml/kg body weight/day.The drug «Karafest + OV Zn» asked orally with food daily for a month to projected pregnancy (140 days of pregnancy).The positive effect of the drug «Karafest + OV Zn» at the homeostasis parameters in sheep, including normalization of serum vitamin A, zinc and total protein; prooxidative-antioxidant system containing serum – malondialdehyde, catalase, superoxide dismutase and erythrocyte – malondialdehyde, catalase, restored glutathione and oxidative-antioxidant value; system state oxygen metabolism – the number of red blood cells and hemoglobin concentration of 2.3-dyphosphoglyceratis.Thus, the use of the drug «Karafest + OV Zn» increased content in blood serum, vitamin A (by 16.2%), zinc (by 23,4%), total protein (by 11.5%), malondialdehyde (by 17.5%), catalase (by 24.7%), superoxide dismutase (by 17.9%); in erythrocytes: (by 24.4%), catalase (by 23.9 %) and reduced glutathione (by 18.2%) and hemoglobin (by 23.5 %), the actual number of red blood cells (by 21.8%), the concentration of 2,3-dyphosphoglyceratis (by 22.2%) and normalized prooxidant-antioxidant ratio.
An LC–MS Assay to Measure Superoxide Radicals and Hydrogen Peroxide in the Blood System
