A new method for in vitro propagation of lilac (Syringa vulgaris L.): regrowth and storage conditions for axillary buds encapsulated in alginate beads, development of a pre-acclimatisation stage

1998 ◽  
Vol 74 (3) ◽  
pp. 233-241 ◽  
Author(s):  
E Refouvelet ◽  
S Le Nours ◽  
C Tallon ◽  
F Daguin
Keyword(s):  
In Vitro Propagation ◽  
Storage Conditions ◽  
Alginate Beads ◽  
Axillary Buds ◽  
New Method ◽  
And Storage

scholarly journals Botanicals to Control Soft Rot Bacteria of Potato

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
M. M. Rahman ◽  
A. A. Khan ◽  
M. E. Ali ◽  
I. H. Mian ◽  
A. M. Akanda ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Curcuma Longa ◽  
Soft Rot ◽  
Storage Condition ◽  
Storage Conditions ◽  
Leaf Extracts ◽  
Corchorus Capsularis ◽  
Rot Disease ◽  
And Storage

Extracts from eleven different plant species such as jute (Corchorus capsularisL.), cheerota (Swertia chiraitaHam.), chatim (Alstonia scholarisL.), mander (Erythrina variegata), bael (Aegle marmelosL.), marigold (Tagetes erecta), onion (Allium cepa), garlic (Allium sativumL.), neem (Azadiracta indica), lime (Citrus aurantifolia), and turmeric (Curcuma longaL.) were tested for antibacterial activity against potato soft rot bacteria,E. carotovorasubsp.carotovora (Ecc)P-138, underin vitroand storage conditions. Previously,EccP-138 was identified as the most aggressive soft rot bacterium in Bangladeshi potatoes. Of the 11 different plant extracts, only extracts from dried jute leaves and cheerota significantly inhibited growth ofEccP-138in vitro. Finally, both plant extracts were tested to control the soft rot disease of potato tuber under storage conditions. In a 22-week storage condition, the treated potatoes were significantly more protected against the soft rot infection than those of untreated samples in terms of infection rate and weight loss. The jute leaf extracts showed more pronounced inhibitory effects onEcc-138 growth both inin vitroand storage experiments.

In vitro propagation of the bay laurel (Laurus nobilis.L) in Morocco

2014 ◽  
Vol 4 (3) ◽  
pp. 96-103
Author(s):  
Abdelali Chourfi ◽  
Tajelmolk Alaoui ◽  
Ghizlane Echchgadda
Keyword(s):  
Seed Germination ◽  
In Vitro Culture ◽  
In Vitro Propagation ◽  
Aerial Part ◽  
Basal Medium ◽  
Axillary Buds ◽  
Laurus Nobilis

Laurus nobilis L. is among the species which are most threatened by massive degradation in Morocco. The multiplication by seed or by cuttings gives very low percentages of recovery that is insufficient to meet the demand of growing market. In vitro culture proves to be a tremendous asset to solve this problem. Our work has focused on the study of seed germination of this species and its multiplication from microcuttings. Finally, we studied the ac-climatization ability of the plantlets resulting from this germination. The study of the germination, via the further measurement of the length of the aerial part and the roots and the number of axillary buds for nine weeks, showed that the MS basal medium was more efficient than media 1/2M.S and WPM. Among the eight tested hormones, IAA yielded the best growth of the plantlets. Hormonal combination of NAA and kinetin resulted into a per-centage of the greatest success in reaching 67 % micropropagation. The study also revealed that the MS basal medium in the presence of the IAA plants can acclimate most easily in two types of substrates with improved development in the peat alone.

In vitro propagation of European aspen (Populus tremula L.) from axillary buds via organogenesis

2009 ◽  
Vol 121 (1) ◽  
pp. 109-112 ◽  
Author(s):  
Špela Peternel ◽  
Karin Gabrovšek ◽  
Nada Gogala ◽  
Marjana Regvar
Keyword(s):  
In Vitro Propagation ◽  
Populus Tremula ◽  
Axillary Buds ◽  
European Aspen

scholarly journals Research on the reproductive biological characteristics of Amomum villosum Lour. and Amomum longiligulare T. L. Wu

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0250335
Author(s):  
Ruipei Yang ◽  
Jie Wang ◽  
Wei Gao ◽  
Ye Jiang ◽  
Jing Su ◽  
...  
Keyword(s):  
Evaluation Method ◽  
Pollen Viability ◽  
Storage Conditions ◽  
Biological Characteristics ◽  
High Humidity ◽  
Artificial Fruit ◽  
Natural Pollination ◽  
Amomum Villosum ◽  
And Storage

Objective To explore the influence of biological characteristics on the yield of Amomum villosum Lour. and Amomum longiligulare T. L. Wu, to find an effective pollen viability evaluation method and storage method to solve the problem of the low yield of Amomum plants. Methods Five germplasm of Amomum plants were used to investigate the effects of the phenological phase, pollen viability, and stigma receptivity on natural and artificial fruit set. Results Amomum longiligulare T. L. Wu showed late flowering, and its natural pollination rate is higher than that of Amomum villosum Lour. In all germplasm, the artificial pollination rate and fruit setting rate are more than 3 times higher than that under natural conditions. Fruits begin to drop seven days after successful pollination, and the fruit drop is basically stable after one month. The hybridization verification showed that TTC method was simpler and more accurate than in vitro germination method. Optimal storage conditions for pollen are 4°C and high humidity. After 36 h of storage, pollen can still be used for artificial cross-pollination or as hybrid parents. Conclusion The special biological characteristics are the fundamental reason for the low natural pollination rate of Amomum plants. The accurate measurement method of Amomum plants pollen is the TTC method, and storage at 4°C and high humidity can increase the yield, which was six times that of the natural yield.

scholarly journals In vitro propagation from axillary buds of the endangered tree Dalbergia congestiflora Pittier (Fabaceae)

2021 ◽  
Author(s):  
Alejandra Hernández-García ◽  
Enrique Ambriz-Parra ◽  
Pablo López-Albarrán ◽  
José Cruz-de León ◽  
Rafael Salgado-Garciglia
Keyword(s):  
In Vitro Propagation ◽  
Axillary Buds ◽  
Endangered Tree

PLATELET TRANSFUSION THERAPY - FUTURE TRENDS AND FEASIBLE GOALS

1987 ◽  
Author(s):  
C Th Smit Sibinga
Keyword(s):  
Platelet Transfusion ◽  
Storage Conditions ◽  
Future Trends ◽  
Transfusion Therapy ◽  
Life Saving ◽  
Immunological Effects ◽  
Alternative Approaches ◽  
And Storage ◽  
The Devil

It is better to know than not to know the devil. Platelet transfusion therapy is one such devil. While the therapy initially is a benefit and might even be life-saving, it often implicates future problems which prohibit optimal efficacy.The factors determining both the beneficial and the prohibitive effects are complex and not seldom masked. Problem areas include the logistics of availability, the prevention of immunological effects and incompatibilities, the prevention of trans- missable diseases and the prediction of clinical efficacy.Efforts for optimising platelet transfusion therapy should focus on safety, purity, potency and clinical efficacy. However, each of these premises has to be outlined with precision in order to allow targetted development.Major areas of attention will be the selection and utilisation of available resources, the options for purification, efforts to optimise processing, preservation and storage conditions, innovation of the in vitro viability concept and alternative approaches to selection in order to increase the predictive value of compatibility testing.

Generation of Kinins during Preparation and Storage of Leukoreduced Platelet Concentrates.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 947-947
Author(s):  
Marie Eve Moreau ◽  
Louis Thibault ◽  
Anik Désormeaux ◽  
François Marceau ◽  
Marie-Joëlle de Grandmont ◽  
...  
Keyword(s):  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Storage System ◽  
Reference Population ◽  
Storage Conditions ◽  
Blood Collection ◽  
Converting Enzyme Inhibitors ◽  
Pharmacological Characterization ◽  
And Storage

Abstract BACKGROUND. Severe hypotensive reactions can occur following transfusion of blood components, particularly with platelets concentrates (PCs) leukoreduced with negatively- charged filters. Bradykinin (BK)-related peptides were proposed as a possible cause of this side-effect. This study evaluated the effects of leukoreduction and storage conditions on the levels of two kinins (BK and des-Arg9-BK) in PCs. METHODS. Whole blood donations (n=35) were processed using current PRP (platelet-rich plasma) procedure to prepare leukoreduced and unfiltered components by Leukotrap® RC-PL Whole Blood Collection, Filtration and Storage System. PCs and plasma were stored for 7 days at 20–24°C with agitation. Levels of BK and des-Arg9-BK were measured by specific enzyme immunoassays and HPLC at day 0, 2, 5 and 7 of storage. Mechanisms potentially responsible for accumulation of BK and des-Arg9-BK were studied. RESULTS. On day 0, kinins were measured in significantly higher concentrations in leukoreduced (BK: 101 ± 157 pg/mL; des-Arg9-BK: 194 ± 191 pg/mL) vs unfiltered PCs (BK: 71 ± 121 pg/mL; des-Arg9-BK: 98 ± 114 pg/mL). During storage, both kinins peaked on day 5, with concentrations higher than 1 ng/mL in 22% of leukoreduced as well as unfiltered PCs. Physicochemical and pharmacological characterization of immunoreactive kinins confirmed their nature. In vitro activation of the contact system of the corresponding platelet-poor plasma (PPP) showed that a high kinin concentration on day 5 of the storage corresponded to a low kinin-forming capacity of plasma. On day 7, BK was no longer elevated presumably due to its degradation and the depletion of kinin-forming capacity of the plasma in stored PCs. The activity of metallopeptidases that metabolize BK-related peptides in plasma from PCs were at levels similar to those recorded in the plasma of a normal reference population and were unaffected by storage. CONCLUSIONS. Our results indicate that filtration and storage conditions of PCs contribute to generation of pharmacologically relevant bradykinin levels that might pose a risk in susceptible patients. The clinical relevance of such high concentrations of kinins in PCs remains to be established but could potentially be significant especially in patients treated with angiotensin I-converting enzyme-inhibitors that affect the pathway of BK degradation.

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