In vitro propagation of the bay laurel (Laurus nobilis.L) in Morocco

2014 ◽  
Vol 4 (3) ◽  
pp. 96-103
Author(s):  
Abdelali Chourfi ◽  
Tajelmolk Alaoui ◽  
Ghizlane Echchgadda
Keyword(s):  
Seed Germination ◽  
In Vitro Culture ◽  
In Vitro Propagation ◽  
Aerial Part ◽  
Basal Medium ◽  
Axillary Buds ◽  
Laurus Nobilis

Laurus nobilis L. is among the species which are most threatened by massive degradation in Morocco. The multiplication by seed or by cuttings gives very low percentages of recovery that is insufficient to meet the demand of growing market. In vitro culture proves to be a tremendous asset to solve this problem. Our work has focused on the study of seed germination of this species and its multiplication from microcuttings. Finally, we studied the ac-climatization ability of the plantlets resulting from this germination. The study of the germination, via the further measurement of the length of the aerial part and the roots and the number of axillary buds for nine weeks, showed that the MS basal medium was more efficient than media 1/2M.S and WPM. Among the eight tested hormones, IAA yielded the best growth of the plantlets. Hormonal combination of NAA and kinetin resulted into a per-centage of the greatest success in reaching 67 % micropropagation. The study also revealed that the MS basal medium in the presence of the IAA plants can acclimate most easily in two types of substrates with improved development in the peat alone.

scholarly journals In Vitro Propagation of Apios americana

HortScience ◽  
1990 ◽  
Vol 25 (11) ◽  
pp. 1439-1440 ◽  
Author(s):  
E.R.M. Wickremesinhe ◽  
W.J. Blackmon ◽  
B.D. Reynolds
Keyword(s):  
Gibberellic Acid ◽  
In Vitro Propagation ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Axillary Buds ◽  
Ms Medium ◽  
Butanoic Acid ◽  
Apios Americana

Shoot proliferation from axillary buds of Apios americana Medikus (apios, groundnut) was obtained on a modified Murashige and Skoog (MS) medium supplemented with 2.22 μm BAP, 0.5 μm IBA, and 3.0 μm GA3. Existed shoots rooted on MS basal medium. About 60% of the rooted plants were successfully established in soil. Chemical names used: 1 H-indole-3-butanoic acid (IBA). gibberellic acid (GA3), N6-benzylaminopurine (BAP).

scholarly journals In Vitro Propagation of Heliconia psittacorum by Bud Culture

HortScience ◽  
1992 ◽  
Vol 27 (5) ◽  
pp. 450-452 ◽  
Author(s):  
M.J. Nathan ◽  
C.J. Goh ◽  
P.P. Kumar
Keyword(s):  
In Vitro Propagation ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Coconut Water ◽  
Axillary Buds ◽  
Ms Medium

A protocol was developed for in vitro propagation of Heliconia psittacorum L.f. by culture of terminal and axillary buds of rhizomes. Cultures were initiated on modified Murashige and Skoog (MS) medium containing 40 μm BA, 150 ml coconut water/liter, 30 g sucrose/liter, and 2 g Gelrite/liter. Shoot multiplication was achieved on the above medium without coconut water, but supplemented with 10 μm BA. Shoots were rooted on MS basal medium and successfully acclimated to greenhouse conditions. Chemical names used: N-(phenylmethyl)-1 H-purin-6-amine (BA).

scholarly journals Micropropagation of Pyracantha coccinea

HortScience ◽  
2017 ◽  
Vol 52 (2) ◽  
pp. 271-273
Author(s):  
Chao Dong ◽  
Xue Li ◽  
Yue Xi ◽  
Zong-Ming Cheng
Keyword(s):  
In Vitro Propagation ◽  
Basal Medium ◽  
Ornamental Plant ◽  
Axillary Buds ◽  
Nodal Segments ◽  
Ms Medium ◽  
Southeast Europe ◽  
Rooting Medium ◽  
Pyracantha Coccinea

Pyracantha coccinea is a thorny evergreen shrub native to southeast Europe to southeast Asia. It is a popular ornamental plant because of its showy bright red fruits and small white flowers. However, in vitro vegetative propagation of P. coccinea has not been studied. Nodal segments with one or two axillary buds (1 to 1.5 cm in length) were cut and disinfected in a solution of 0.1% (v/v) mercuric chloride (HgCl2) for 5 minutes, and proliferated on Murashige and Skoog (MS) basal medium supplemented with various concentrations 6-benzylaminopurine (6-BA). After 4 weeks, newly formed shoots were transferred to proliferation and rooting media containing various concentrations of indole-3-butyric acid (IBA). Establishment of axillary buds was significantly better with an establishing rate of 67% on basal MS medium augmented with 6.6 µm 6-BA. The best medium for proliferation of shoots was three-fourth basal MS supplemented with 1.5 µm IBA, with a proliferation rate of 3.4 axillary bud. The optimum rooting medium was one-fourth MS basal medium containing 93 µm IBA. Rooting of shoots was as much as 77%. Rooted plantlets were transferred to pots containing vermiculite:perlite:peat (6:1:2) and acclimatized to ambient greenhouse conditions with a 95% survival rate. This protocol can be used for in vitro propagation of P. coccinea.

scholarly journals Seed Germination and in vitro Plant Regeneration through Callus Culture of Two Lychnis Species

2015 ◽  
Vol 25 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Kee Hwa Bae ◽  
Eui Soo Yoon
Keyword(s):  
Plant Regeneration ◽  
Seed Germination ◽  
Callus Induction ◽  
In Vitro Propagation ◽  
Ornamental Plants ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Temperature Treatment ◽  
In Vitro Plant Regeneration

Lychnis cognate Maxim and Lychnis fulgens Fish. Ex Spreng are two valued ornamental plants in Korea. Soaking of seeds in GA3 solution remarkably promoted germination up to 60%, but the control (0 mg/l) was not effective (> 5%). To select an adequate temperature for seed germination, seeds, previously soaked in a 1000 mg/l GA3 for 24 hrs, were incubated at 15, 20, 25, and 30°C. Seed germination of over 20% was obtained at 15, 20, and 25°C, but only 10% at 30°C. These results indicate that the seeds of L. cognate and L. fulgens are in a such dormant state that they hardly germinate even by dormancy breaker (GA3) and low (15 ? 25°C) temperature treatment. The highest callus induction was observed in the leaf explants of the seedlings on MS containing specific concentrations of 3.0 mg/l BA and 1.0 mg/l NAA. The adventitious shoot was formed < 90% of calli on 1/2 WPM medium. The height of in vitro propagated plantlet was no different media used for regeneration. This in vitro propagation protocol should be useful for conservation of endangered and ornamental plant.Plant Tissue Cult. & Biotech. 25(1): 1-12, 2015 (June)

In vitro propagation of Crambe maritima

1987 ◽  
Vol 65 (1) ◽  
pp. 72-75 ◽  
Author(s):  
J. Y. Peron ◽  
E. Regnier
Keyword(s):  
In Vitro Propagation ◽  
Indoleacetic Acid ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Naphthaleneacetic Acid ◽  
Petiole Explants ◽  
Acclimatization Period

A method for rapid micropropagation of sea kale (Crambe maritima L.) was developed. Petiole explants placed in vitro on a medium containing 0.5 mg/L indoleacetic acid (IAA), 6.0 mg/L kinetin, and 1.5 mg/L benzylaminopurine developed callus within 15 days and shoots within 28 days. Nearly four adventitious shoots could be developed within 3 weeks by placing the initial shoot on media without IAA. To develop roots, the shoots were then transferred to the basal medium containing 0.1 to 1.0 mg/L indolbutyric or α-naphthaleneacetic acid. Rooted plantlets were obtained within 2 or 3 weeks. After an acclimatization period of 6 weeks in a greenhouse in unsterilized medium, the plantlets could be set outdoors.

Brassinolide on In Vitro Growth of Apical Meristem of Bananas

2014 ◽  
Vol 8 (1) ◽  
Author(s):  
FLORENDA C. BALLESTEROS-TEMANEL
Keyword(s):  
Plant Growth ◽  
In Vitro Propagation ◽  
Apical Meristem ◽  
Basal Medium ◽  
Tissue Growth ◽  
Shoot Length ◽  
New Class ◽  
Randomized Design ◽  
Plant Growth Substances

Brassinosteroids (BRs) are new class of hormones noted to perform multiplephysiological functions in plant growth and development and have the potentialof influencing cell and tissue growth in vitro. Many naturally occurring BRs,including brassinolide, have been discovered, their mode of action and their growthpromoting activities on plants. The use of brassinolide in in vitro propagation isnew. The Murashige and Skoog (1962) medium was used as basal medium. Plantgrowth regulators - IAA, BA and BR - were added to the medium. The study usedthe Completely Randomized Design (CRD) in factorial with three replications.The cultivar of banana and plant growth substances affected the number of budsproduced, shoot length, root length, and stem girth. The interaction of thesetwo factors (cultivar x PGR) influenced the number of buds produced in vitroand the shoot length of the meriplants. The study shows that brassinolide has aninfluence on shoot induction, proliferation, and elongation of bananas in in vitro propagation.Keywords: Agriculture, in vitro propagation, induction, proliferation, elongation, apical meristem, plant growth regulators, cultivars, Isabela, Philippines

In vitro propagation of European aspen (Populus tremula L.) from axillary buds via organogenesis

2009 ◽  
Vol 121 (1) ◽  
pp. 109-112 ◽  
Author(s):  
Špela Peternel ◽  
Karin Gabrovšek ◽  
Nada Gogala ◽  
Marjana Regvar
Keyword(s):  
In Vitro Propagation ◽  
Populus Tremula ◽  
Axillary Buds ◽  
European Aspen

scholarly journals Micropropagation of Cypripedium formosanum Hayata through Axillary Buds from Mature Plants

HortScience ◽  
2010 ◽  
Vol 45 (9) ◽  
pp. 1369-1372 ◽  
Author(s):  
Yung-I Lee
Keyword(s):  
Average Length ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Axillary Buds ◽  
Orchid Species ◽  
Optimum Result ◽  
Aseptic Culture ◽  
Slipper Orchid

A micropropagation protocol for an endangered slipper orchid species, Cypripedium formosanum Hayata, through axillary buds from adult plants has been developed. The season of explant collection is crucial for the initial success of an aseptic culture. Explants collected in the middle of January gave the highest percentage of explant survival (54.2%) and shoot-forming percentage (41.7%). Of the two cytokinins tested, N6-benzyladenine (BA) was found to be superior to thidiazuron for normal shoot formation. The optimum result was obtained in quarter-strength Murashige and Skoog medium containing 22.2 or 44.4 μM BA in which the cultures produced 6.3 and 7.1 shoots per explant with 10.6 to 11.7 mm average length after 90 d of culture. Regenerated shoots rooted for 60 d in the basal medium with 1 g·L−1 activated charcoal and 20 g·L−1 potato homogenate were ready for growth in pots. This is the first report on shoot multiplication in vitro from mature plants of Cypripedium that provides a reliable method for propagating the selected elites.

scholarly journals In vitro propagation from axillary buds of the endangered tree Dalbergia congestiflora Pittier (Fabaceae)

2021 ◽  
Author(s):  
Alejandra Hernández-García ◽  
Enrique Ambriz-Parra ◽  
Pablo López-Albarrán ◽  
José Cruz-de León ◽  
Rafael Salgado-Garciglia
Keyword(s):  
In Vitro Propagation ◽  
Axillary Buds ◽  
Endangered Tree
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