2048 Prevention of irradiation-induced apoptosis in human esophagus sections in vitro by overexpression of the manganese superoxide dismutase transgene

1999 ◽  
Vol 45 (3) ◽  
pp. 302
Author(s):  
J.A. Bray ◽  
M.W. Epperly ◽  
S. Defillipi ◽  
G. Koe ◽  
D. Liggitt ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Manganese Superoxide Dismutase ◽  
Manganese Superoxide ◽  
Human Esophagus ◽  
Induced Apoptosis

scholarly journals Manganese superoxide dismutase as a target of autoantibodies in acute Epstein-Barr virus infection.

1994 ◽  
Vol 180 (5) ◽  
pp. 1995-1998 ◽  
Author(s):  
K Ritter ◽  
R J Kühl ◽  
F Semrau ◽  
H Eiffert ◽  
H D Kratzin ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Epstein Barr Virus ◽  
Manganese Superoxide Dismutase ◽  
Clinical Symptoms ◽  
Epstein Barr Virus Infection ◽  
Barr Virus ◽  
Ebv Infection ◽  
Manganese Superoxide ◽  
Epstein Barr

Antibodies directed against the autoantigen p26 were detected in sera from 32 patients with acute Epstein-Barr virus (EBV) infection and clinical symptoms of infectious mononucleosis. P26 has now been identified as the enzyme manganese superoxide dismutase (MnSOD) by comparison of the NH2-terminal amino acid sequence. Antibodies against MnSOD belong to the immunoglobulin class M. They are not detectable in sera of patients with other herpesvirus infections. In the 32 patients investigated, the rise and fall of the autoantibodies coincides with the clinical symptoms. In vitro, the autoantibodies were shown to inhibit the dismutation of superoxide radicals by blocking MnSOD. As presented in the discussion this effect may contribute to the pathogenesis of acute EBV infection.

Manganese Superoxide Dismutase Protects from TNF-α–Induced Apoptosis by Increasing the Steady-State Production of H2O2

2006 ◽  
Vol 8 (7-8) ◽  
pp. 1295-1305 ◽  
Author(s):  
Jaya Dasgupta ◽  
Sita Subbaram ◽  
Kip M. Connor ◽  
Ana M. Rodriguez ◽  
Oren Tirosh ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Steady State ◽  
Manganese Superoxide Dismutase ◽  
Manganese Superoxide ◽  
Tnf Α ◽  
State Production ◽  
Induced Apoptosis

scholarly journals Radioprotection In Vitro and In Vivo by Minicircle Plasmid Carrying the Human Manganese Superoxide Dismutase Transgene

2008 ◽  
Vol 19 (8) ◽  
pp. 820-826 ◽  
Author(s):  
Xichen Zhang ◽  
Michael W. Epperly ◽  
Mark A. Kay ◽  
Zhi-Ying Chen ◽  
Tracy Dixon ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Manganese Superoxide Dismutase ◽  
Manganese Superoxide

In vitro cell free synthesis of human manganese superoxide dismutase with the RTS 500 system

2003 ◽  
Vol 786 (1-2) ◽  
pp. 137-142
Author(s):  
Michael Kleines ◽  
Martin Nellessen ◽  
Lars Schaade ◽  
Klaus Ritter
Keyword(s):  
Superoxide Dismutase ◽  
Manganese Superoxide Dismutase ◽  
Manganese Superoxide

scholarly journals Antioxidant Effect of Soymilk Fermented by Lactobacillus plantarum HFY01 on D-Galactose-Induced Premature Aging Mouse Model

2021 ◽  
Vol 8 ◽  
Author(s):  
Chong Li ◽  
Yang Fan ◽  
Shuang Li ◽  
Xianrong Zhou ◽  
Kun-Young Park ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Lactobacillus Plantarum ◽  
High Performance ◽  
Manganese Superoxide Dismutase ◽  
Antioxidant Effect ◽  
Premature Aging ◽  
Lactobacillus Bulgaricus ◽  
Manganese Superoxide ◽  
Genes Encoding

The antioxidant effect of soymilk fermented by Lactobacillus plantarum HFY01 (screened from yak yogurt) was investigated on mice with premature aging induced by D-galactose. In vitro antioxidant results showed that L. plantarum HFY01-fermented soymilk (LP-HFY01-DR) had better ability to scavenge the free radicals 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS) than unfermented soymilk and Lactobacillus bulgaricus-fermented soymilk. Histopathological observation showed that LP-HFY01-DR could protect the skin, spleen and liver, reduce oxidative damage and inflammation. Biochemical results showed that LP-HFY01-DR could effectively upregulate glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) levels and decrease malondialdehyde (MDA) content in the liver, brain, and serum. Real-time quantitative reverse transcription polymerase chain reaction further showed that LP-HFY01-DR could promote the relative expression levels of the genes encoding for cuprozinc superoxide dismutase (Cu/Zn-SOD, SOD1), manganese superoxide dismutase (Mn-SOD, SOD2), CAT, GSH, and GSH-Px in the liver, spleen, and skin. High-performance liquid chromatography results revealed daidzin, glycitin, genistin, daidzein, glycitein, and genistein in LP-HFY01-DR. In conclusion, LP-HFY01-DR could improve the antioxidant capacity in mice with premature aging induced by D-galactose.

scholarly journals SUPPRESSION OF MANGANESE SUPEROXIDE DISMUTASE ACTIVITY IN ROTENONE-TREATED HUMAN GLIOBLASTOMA T98G CELLS REDUCES CELL VIABILITY

2018 ◽  
Vol 11 (1) ◽  
pp. 48
Author(s):  
Septelia Inawati Wanandi ◽  
Novi Silvia Hardiany ◽  
Nurjati Chairani Siregar ◽  
Mohamad Sadikin
Keyword(s):  
Superoxide Dismutase ◽  
Cell Viability ◽  
Manganese Superoxide Dismutase ◽  
Specific Activity ◽  
High Dose ◽  
Human Glioblastoma ◽  
Manganese Superoxide ◽  
Rotenone Treatment ◽  
Intracellular Ros

  Objective: Glioma is the most common human primary brain tumor which is highly resistant to oxidative stress-based anticancer. The aim of this study was to analyze the effect of rotenone-induced reactive oxygen species (ROS) on the modulation of manganese superoxide dismutase (MnSOD) expression and cell viability in human glioblastoma (GBM) T98G cells.Methods: In this in vitro experimental study, T98G cells were treated with high-dose rotenone (0.5, 5, and 50 μM, respectively). Following rotenone treatment and intracellular ROS, both peroxide and superoxide radicals were determined. Moreover, we analyzed MnSOD mRNA expression, protein, and specific activity, as well as cell survival including viability, proliferation, apoptosis, and mitochondrial structure.Results: High-dose rotenone treatment of T98G cells significantly increased intracellular ROS and MnSOD mRNA, but its protein and specific activity definitely decreased. The treatment also led to a reduction of cell viability, enhancement of apoptosis, and disruption of mitochondrial integrity.Conclusion: Overproduction of ROS in rotenone-treated human GBM T98G cells could suppress the MnSOD protein level and activity even though mRNA synthesis has been increased. This modulation led to reduced survival of T98G cells through induction of cell death rather than inhibition of cell proliferation.

scholarly journals The suppression of manganese superoxide dismutase decreased the survival of human glioblastoma multiforme T98G cells

2017 ◽  
Vol 26 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Novi S. Hardiany ◽  
Mohamad Sadikin ◽  
Nurjati Siregar ◽  
Septelia I. Wanandi
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Glioblastoma Multiforme ◽  
Manganese Superoxide Dismutase ◽  
Superoxide Radical ◽  
Specific Activity ◽  
Human Glioblastoma Multiforme ◽  
Manganese Superoxide ◽  
In Vitro Transfection

Background: Glioblastoma multiforme (GBM) is a primary malignant brain tumor which has poor prognosis. High incidence of oxidative stress-based therapy resistance could be related to the high antioxidant status of GBM cells. Our previous study has reported that manganese superoxide dismutase (MnSOD) antioxidant expression was significantly higher in high grade glioma than in low grade. The aim of this study was to analyze the impact of MnSOD suppression toward GBM cell survival.Methods: This study is an experimental study using human glioblastoma multiforme T98G cell line. Suppression of MnSOD expression was performed using in vitro transfection MnSOD-siRNA. The MnSOD expression was analyzed by measuring the mRNA using real time RT-PCR, protein using ELISA technique, and specific activity of enzyme using inhibition of xantine oxidase. Concentration of reactive oxygen species (ROS) intracellular was determined by measuring superoxide radical and hydrogen peroxide. Cell survival was analyzed by measuring viability, proliferation, and cell apoptosis.Results: In vitro transfection of MnSOD-siRNA suppressed the mRNA, protein, and specific activity of MnSOD. This treatment significantly increased the concentration of superoxide radical; however, it did not influence the concentration of hydrogen peroxide. Moreover, viability MnSOD-suppressing cell significantly decreased, accompanied by increase of cell apoptosis without affecting cell proliferation.Conclusion: The suppression of MnSOD expression leads to decrease glioblastoma multiforme cell survival, which was associated to the increase of cell apoptotic.

Sign in / Sign up

Export Citation Format

Share Document