Cloning and characterization of the ?-cysteine desulfhydrase gene of Fusobacterium nucleatum

2002 ◽  
Vol 215 (1) ◽  
pp. 75-80 ◽  
Author(s):  
H Fukamachi
Keyword(s):  
Fusobacterium Nucleatum ◽  
Cysteine Desulfhydrase

scholarly journals Characterization of the Subgingival Cultivable Microbiota in Patients with Different Stages of Periodontitis in Spain and Colombia. A Cross-Sectional Study

2021 ◽  
Vol 9 (9) ◽  
pp. 1940
Author(s):  
Roquelina Pianeta ◽  
Margarita Iniesta ◽  
Diana Marcela Castillo ◽  
Gloria I. Lafaurie ◽  
Mariano Sanz ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Fusobacterium Nucleatum ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Chi Square ◽  
Cross Sectional ◽  
Campylobacter Rectus ◽  
Parvimonas Micra ◽  
Final Sample

The objective was to characterize and compare the subgingival microbiota in patients diagnosed according to the World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions 2018. For this cross-sectional study, Spanish and Colombian subjects (characterized as health/gingivitis, periodontitis in stages I-II or stages III-IV) were clinically assessed, and subgingival samples were taken and processed by culture. The comparisons among patients with periodontal status (and between countries) was made using Mann–Whitney, Kruskal–Wallis, ANOVA and chi-square tests. The final sample consisted of 167 subjects. Eikenella corrodens and Parvimonas micra were more frequently detected in health/gingivitis and Porphyromonas gingivalis in periodontitis (p < 0.05). Higher total counts were observed in Colombia (p = 0.036). In Spain, significantly higher levels of P. gingivalis and Campylobacter rectus were observed, and of Tannerella forsythia, P. micra, Prevotella intermedia, Fusobacterium nucleatum, Actinomyces odontolyticus and Capnocytophaga spp. in Colombia (p < 0.001). P. micra was more prevalent in health/gingivitis and stage I-II periodontitis in Colombia, and P. gingivalis in all periodontitis groups in Spain (p < 0.05). As conclusions, significant differences were detected in the microbiota between health/gingivitis and periodontitis, with minor differences between stages of periodontitis. Differences were also relevant between countries, with Colombia showing larger counts and variability of bacterial species.

scholarly journals Identification and Characterization of Fusolisin, the Fusobacterium nucleatum Autotransporter Serine Protease

PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e111329 ◽  
Author(s):  
Lior Doron ◽  
Shunit Coppenhagen-Glazer ◽  
Yara Ibrahim ◽  
Amir Eini ◽  
Ronit Naor ◽  
...  
Keyword(s):  
Serine Protease ◽  
Fusobacterium Nucleatum ◽  
Identification And Characterization

Biochemical characterization of serine acetyltransferase and cysteine desulfhydrase from Leishmania major

2010 ◽  
Vol 173 (2) ◽  
pp. 170-174 ◽  
Author(s):  
Daniela Marciano ◽  
Marianela Santana ◽  
Brian Suárez Mantilla ◽  
Ariel Mariano Silber ◽  
Cristina Marino-Buslje ◽  
...  
Keyword(s):  
Leishmania Major ◽  
Biochemical Characterization ◽  
Serine Acetyltransferase ◽  
Cysteine Desulfhydrase

scholarly journals Patients with mesenchymal tumours and high Fusobacteriales prevalence have worse prognosis in colorectal cancer (CRC)

2021 ◽  
Author(s):  
Manuela Salvucci ◽  
Nyree T Crawford ◽  
Katie Stott ◽  
Susan Bullman ◽  
Daniel B Longley ◽  
...  
Keyword(s):  
Logistic Models ◽  
Fusobacterium Nucleatum ◽  
Molecular Subtyping ◽  
Cell Type Composition ◽  
Type Composition ◽  
Host Signaling ◽  
Context Specific ◽  
Mesenchymal Tumours

Objective: Transcriptomic-based subtyping, Consensus Molecular Subtyping (CMS) and CRC Intrinsic Subtyping (CRIS), identify a patient subpopulation with mesenchymal traits (CMS4/CRIS-B) and poorer outcome. Here, we investigated the relationship between prevalence of Fusobacterium nucleatum (Fn) and Fusobacteriales, CMS/CRIS subtyping, cell type composition, immune infiltrates and host contexture to refine patients stratification and identify druggable context-specific vulnerabilities. Design: We coupled cell culture experiments with characterization of Fn/Fusobacteriales prevalence and host biology/microenviroment in tumours from 2 independent CRC patient cohorts (Taxonomy: n=140; TCGA-COAD-READ: n=605). Results: In vitro, Fn infection induced inflammation via NFκB/TNFα in HCT116 and HT29 cancer cell lines. In patients, high Fn/Fusobacteriales were found in CMS1, MSI tumours, with infiltration of macrophages M1, reduced macrophages M2, and high IL6/IL8/IL1β signaling. Analysis of the Taxonomy cohort suggested that Fn was prognostic for CMS4/CRIS-B patients, despite having lower Fn load than CMS1 patients. In the TCGA-COAD-READ cohort, we likewise identified a differential association between Fusobacteriales relative abundance and outcome when stratifying patients in mesenchymal (either CMS4 and/or CRIS-B) vs. non-mesenchymal (neither CMS4 nor CRIS-B). Patients with mesenchymal tumours and high Fusobacteriales had approximately 2-fold higher risk of worse outcome. These associations were null in non-mesenchymal patients. Modelling the 3-way association between Fusobacteriales prevalence, molecular subtyping, and host contexture with logistic models with an interaction term disentangled the pathogen/host-signaling relationship and identified aberrations (including EMT/WNT/NOTCH) as candidate targets. Conclusion: This study identifies CMS4/CRIS-B patients with high Fn/Fusobacteriales prevalence as a high-risk subpopulation that may benefit from therapeutics targeting mesenchymal biology.

scholarly journals Genetic and Biochemical Characterization of OXA-63, a New Class D β-Lactamase from Brachyspira pilosicoli BM4442

2008 ◽  
Vol 52 (4) ◽  
pp. 1264-1268 ◽  
Author(s):  
Djalal Meziane-Cherif ◽  
Thierry Lambert ◽  
Marine Dupêchez ◽  
Patrice Courvalin ◽  
Marc Galimand
Keyword(s):  
Escherichia Coli ◽  
Clavulanic Acid ◽  
Inhibitory Concentration ◽  
Biochemical Characterization ◽  
Fusobacterium Nucleatum ◽  
Cloning And Sequencing ◽  
Brachyspira Pilosicoli ◽  
New Class ◽  
Class D

ABSTRACT Brachyspira pilosicoli BM4442, isolated from the feces of a patient with diarrhea at the Hospital Saint-Michel in Paris, was resistant to oxacillin (MIC > 256 μg/ml) but remained susceptible to cephalosporins and to the combination of amoxicillin and clavulanic acid. Cloning and sequencing of the corresponding resistance determinant revealed a coding sequence of 807 bp encoding a new class D β-lactamase named OXA-63. The bla OXA-63 gene was chromosomally located and not part of a transposon or of an integron. OXA-63 shared 54% identity with FUS-1 (OXA-85), an oxacillinase from Fusobacterium nucleatum subsp. polymorphum, and 25 to 44% identity with other class D β-lactamases (DBLs) and contained all the conserved structural motifs of DBLs. Escherichia coli carrying the bla OXA-63 gene exhibited resistance to benzylpenicillin and amoxicillin but remained susceptible to amoxicillin in combination with clavulanic acid. Mature OXA-63 consisted of a 31.5-kDa polypeptide and appeared to be dimeric. Kinetic analysis revealed that OXA-63 exhibited a narrow substrate profile, hydrolyzing oxacillin, benzylpenicillin, and ampicillin with catalytic efficiencies of 980, 250, and 150 mM−1 s−1, respectively. The enzyme did not apparently interact with oxyimino-cephalosporins, imipenem, or aztreonam. Unlike FUS-1 and other DBLs, OXA-63 was strongly inhibited by clavulanic acid (50% inhibitory concentration [IC50] of 2 μM) and tazobactam (IC50 of 0.16 μM) and exhibited low susceptibility to NaCl (IC50 of >2 M). OXA-63 is the first DBL described for the anaerobic spirochete B. pilosicoli.

Sign in / Sign up

Export Citation Format

Share Document