Characterization of the Subgingival Cultivable Microbiota in Patients with Different Stages of Periodontitis in Spain and Colombia. A Cross-Sectional Study

The objective was to characterize and compare the subgingival microbiota in patients diagnosed according to the World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions 2018. For this cross-sectional study, Spanish and Colombian subjects (characterized as health/gingivitis, periodontitis in stages I-II or stages III-IV) were clinically assessed, and subgingival samples were taken and processed by culture. The comparisons among patients with periodontal status (and between countries) was made using Mann–Whitney, Kruskal–Wallis, ANOVA and chi-square tests. The final sample consisted of 167 subjects. Eikenella corrodens and Parvimonas micra were more frequently detected in health/gingivitis and Porphyromonas gingivalis in periodontitis (p < 0.05). Higher total counts were observed in Colombia (p = 0.036). In Spain, significantly higher levels of P. gingivalis and Campylobacter rectus were observed, and of Tannerella forsythia, P. micra, Prevotella intermedia, Fusobacterium nucleatum, Actinomyces odontolyticus and Capnocytophaga spp. in Colombia (p < 0.001). P. micra was more prevalent in health/gingivitis and stage I-II periodontitis in Colombia, and P. gingivalis in all periodontitis groups in Spain (p < 0.05). As conclusions, significant differences were detected in the microbiota between health/gingivitis and periodontitis, with minor differences between stages of periodontitis. Differences were also relevant between countries, with Colombia showing larger counts and variability of bacterial species.

Microbiological study of periodontal disease in populations with HIV: a systematic review and meta-analysis

Background: No systematic review/meta-analysis has been conducted on the microbiological profile associated with the occurrence of periodontitis in patients with HIV. The aim of this study was to evaluate the prevalence of identified bacteria in HIV-infected patients with periodontal disease.Methods: Three English electronic databases (MEDLINE (via PubMed), SCOPUS, and Web of Science) were searched systematically from the beginning to 13 February 2021. The frequency of each identified bacteria in HIV-infected patients with periodontal disease was extracted. All meta-analysis methods were performed using STATA software. Results: Twenty-two articles met inclusion criteria and enrolled into the systematic review. This review analyzed a total of 965 HIV-infected patients with periodontitis. The prevalence of periodontitis was higher in HIV-infected male patients (83% (CI95%: 76-88%)) compared to females (28% (CI95%: 17-39%)). In our study, the pooled prevalence of necrotizing ulcerative periodontitis and necrotizing ulcerative gingivitis in patients with HIV infection was 67% (CI95%: 52-82%) and 60% (CI95%: 45-74%), while a lower prevalence of linear gingivitis erythema was reported (11% (CI95%: 5-18%)). More than 140 bacterial species were identified from HIV-infected patients with periodontal disease. High prevalence of Tannerella forsythia (51% (CI95%: 5-96%)), Fusobacterium nucleatum (50% (CI95%: 21-78%)), Prevotella intermedia (50% (CI95%: 32-68%)), Peptostreptococcus micros (44% (CI95%: 25-65%)), Campylobacter rectus (35% (CI95%: 25-45%)), and Fusobacterium spp. (35% (CI95%: 3-78%)) in HIV-infected patients with periodontal disease was found.Conclusion: Our study demonstrated that the prevalence of red and orange complex of bacteria in HIV patients with periodontal disease is relatively high.

DETECTION OF BACTERIAL SPECIES ASSOCIATED WITH PERIODONTAL DISEASE IN A GROUP OF PATIENTS

Objectives. The study aimed to detect the presence/quantification of the 12 bacterial strains more frequently involved in the occurrence and aggravation of periodontal disease in a group of patients. Material and method. The study included 55 patients of both sexes, from the portfolio of the „Dr. Vodiță“ Dental Clinic, with chronic marginal periodontitis, who had not been under regular dental control and hygiene for at least 2 years. During the first treatment session, fluid was collected from each patient from the periodontal bags using sterile paper cones from the collection putty. Subsequently, fluid-soaked paper cones from periodontal bags were sent to the Genetic Lab in Bucharest for DNA extraction and quantification of 12 bacterial species, more common in the etiology of periodontal disease. For the correctness of the results obtained, it is necessary that patients have not taken antibiotics in the last 3 weeks. The data were analyzed and statistically processed with the Microsoft Excel 2016 program. Results. The least common bacteria found were from the species: Capnocytophaga ochracea, Aggregatibacter actinomycetemcomitans, Eikanella corrodens and Campylobacter rectus, closely followed by Capnocytophaga gingivalis, Prevotella intermedia and Capnocytophaga sputigena. The rest of the bacterial species were detected much more frequently. Discussions. In the analyzed group there was no monoinfection with Aggregatibacter actinomycetemcomitans. Porphyromonas gingivalis had an important presence in the studied group, registering increased levels in 89% of cases. In our group, Treponema denticola was present in increased amounts in 76.36% of cases. Tannerella forsythia was present in 92.72% of cases in elevated concentrations. Eikanella corrodens and Campylobacter rectus were present in 18.18% of the cases studied in high quantities. Prevotella intermedia was present in significant amounts in 34.54% of cases. Fusobacterium nucleatum was present in high concentrations in 98.18% of the cases studied. Prevotella nigrescens was present in increased amounts in 56.36% of cases. Capnocytophaga ochracea was present in 9.09% of cases in high amounts, Capnocytophaga sputigena in 38.18% of cases, and Capnocytophaga gingivalis was detected in 24.45% of cases. Conclusions. From the class of bacteria with high pathogenicity, we most frequently encountered in the studied group Tannerella forsythia. From the class of bacteria with moderate pathogenicity, we encountered the most common Fusobacterium nucleatum. From the class of bacteria with low pathogenicity, we encountered the most common Capnocytophaga sputigena. In order to validate the results obtained, it is necessary to extend the study to a larger number of patients.

MICRO BIOLOGY OF PERIODONTAL DISEASE- A REVIEW

Periodontal diseases are inflammatory and destructive diseases of the dentogingival complex associated with specific periodontal pathogens inhabiting periodontal pockets. Periodontal diseases lead to damage of the periodontal tissues supporting the teeth (bone and connective tissue) and affect the quality of life of the affected individuals: poor alimentation, tooth loss, social and financial problems. Although it is generally considered that the disease has multifactorial etiology, data show that some specific Gram-negative microorganisms in the subgingival plaque biofilm play a major role in the initiation and progression of periodontitis. Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia form a consortium in the subgingival biofilm and are regarded as the principal periodontopathogenic bacteria. Other microorganisms that have been implicated as predominant species in the disease process are: Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Prevotella intermedia, Campylobacter rectus, Peptostreptococcus migros, Eikenella corrodens. In periodontitis, the initiation of the disease is the colonization of the tissues by these pathogenic species. The next step is bacterial invasion or invasion by pathogenic products into the periodontal tissues, interactions of bacteria or their substances with host cells, and this directly/indirectly causes degradation of the periodontium, resulting in tissue destruction. Keywords: periodontal disease, periodontal pathogens, microbiology.

Efficacy of Aloe Vera Hydrogel in Inhibition The Growth of Campylobacter rectus and Provetella intermedia Bacteria

Introduction: Campylobacter rectus and Provetella intermedia bacteria as the main etiology causes of periodontal disease. One of the locally synthesized antimicrobials that is widely used for the treatment of periodontitis is Chlorhexidine, however this drug has side effects when used continuously. To overcome this, an alternative periodontitis therapy with medicinal plants is needed. This study aims to determine the effectiveness of hydrogel Aloe vera antibacterial concentrations of 2.5%, 5%, 10% and 20% in the bacteria Campylobacter rectus and Provetella intermedia. Methods: This study was a laboratory experimental study with posttest only control group design in vitro. The samples were Campylobacter rectus and Provetella intermedia. The number of groups in this study was six. The sample size was determined by Federer's formula and obtained four replications for each group. Data collection by measuring the inhibition diameter using a slide caliper. Data were analyzed using oneway ANOVA and posthoc LSD. Results: The results showed that there was a difference in the diameter of the hydrogel Aloe vera inhibition with a concentration of 2.5%, 5%, 10%, 20% and a positive control for Campylobacter rectus and Provetella intermedia bacteria. Conclusion: This study concluded that there is an antibacterial effectiveness of hydrogel Aloe vera in concentrations of 2.5%, 5%, 10% and 20% on Campylobacter rectus and Provetella intermedia bacteria.

Efficacy of Aloe Vera Hydrogel in Inhibition The Growth of Campylobacter rectus and Provetella intermedia Bacteria

Introduction: Campylobacter rectus and Provetella intermedia bacteria as the main etiology causes of periodontal disease. One of the locally synthesized antimicrobials that is widely used for the treatment of periodontitis is Chlorhexidine, however this drug has side effects when used continuously. To overcome this, an alternative periodontitis therapy with medicinal plants is needed. This study aims to determine the effectiveness of hydrogel Aloe vera antibacterial concentrations of 2.5%, 5%, 10% and 20% in the bacteria Campylobacter rectus and Provetella intermedia. Methods: This study was a laboratory experimental study with posttest only control group design in vitro. The samples were Campylobacter rectus and Provetella intermedia. The number of groups in this study was six. The sample size was determined by Federer's formula and obtained four replications for each group. Data collection by measuring the inhibition diameter using a slide caliper. Data were analyzed using oneway ANOVA and posthoc LSD. Results: The results showed that there was a difference in the diameter of the hydrogel Aloe vera inhibition with a concentration of 2.5%, 5%, 10%, 20% and a positive control for Campylobacter rectus and Provetella intermedia bacteria. Conclusion: This study concluded that there is an antibacterial effectiveness of hydrogel Aloe vera in concentrations of 2.5%, 5%, 10% and 20% on Campylobacter rectus and Provetella intermedia bacteria.