The African trypanosome cyclophilin A homologue contains unusual conserved central and N-terminal domains and is developmentally regulated

Gene ◽  
2002 ◽  
Vol 290 (1-2) ◽  
pp. 181-191 ◽  
Author(s):  
Roger Pellé ◽  
Francis McOdimba ◽  
Francis Chuma ◽  
Delia Wasawo ◽  
Terry W Pearson ◽  
...  
Keyword(s):  
Cyclophilin A ◽  
Developmentally Regulated ◽  
African Trypanosome ◽  
Terminal Domains

scholarly journals Developmentally regulated cytokeratin gene in Xenopus laevis.

1985 ◽  
Vol 5 (10) ◽  
pp. 2575-2581 ◽  
Author(s):  
J A Winkles ◽  
T D Sargent ◽  
D A Parry ◽  
E Jonas ◽  
I B Dawid
Keyword(s):  
Amino Acid ◽  
Xenopus Laevis ◽  
Intermediate Filament ◽  
Type I ◽  
Developmentally Regulated ◽  
Amino Acid Homology ◽  
Intermediate Filament Proteins ◽  
Functional Roles ◽  
Terminal Domain ◽  
Terminal Domains

We have determined the sequence of cloned cDNAs derived from a 1,665-nucleotide mRNA which transiently accumulates during Xenopus laevis embryogenesis. Computer analysis of the deduced amino acid sequence revealed that this mRNA encodes a 47-kilodalton type I intermediate filament subunit, i.e., a cytokeratin. As is common to all intermediate filament subunits so far examined, the predicted polypeptide, named XK70, contains N- and C-terminal domains flanking a central alpha-helical rod domain. The overall amino acid homology between XK70 and a human 50-kilodalton type I keratin is 47%; homology within the alpha-helical domain is 57%. The N-terminal domain, which is not completely contained in our cDNAs, is basic, contains 42% serine plus alanine, and includes five copies of a six-amino-acid repeating unit. The C-terminal domain has a high alpha-helical content and contains a region with sequence homology to the C-terminal domains of other type I and type III intermediate filament proteins. We suggest that different keratin filament subtypes may have different functional roles during amphibian oogenesis and embryogenesis.

scholarly journals The Host-Pathogen interaction of human cyclophilin A and HIV-1 Vpr requires specific N-terminal and novel C-terminal domains

2011 ◽  
Vol 11 (1) ◽  
pp. 49 ◽  
Author(s):  
Sara MØ Solbak ◽  
Victor Wray ◽  
Ole Horvli ◽  
Arnt J Raae ◽  
Marte I Flydal ◽  
...  
Keyword(s):  
Cyclophilin A ◽  
Host Pathogen Interaction ◽  
Host Pathogen ◽  
Hiv 1 ◽  
Terminal Domains

scholarly journals The DNA damage response is developmentally regulated in the African trypanosome

DNA Repair ◽  
2019 ◽  
Vol 73 ◽  
pp. 78-90 ◽  
Author(s):  
J.P. Vieira-da-Rocha ◽  
D.G. Passos-Silva ◽  
I.C. Mendes ◽  
E.A. Rocha ◽  
D.A. Gomes ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Damage Response ◽  
Developmentally Regulated ◽  
African Trypanosome ◽  
Damage Response

scholarly journals Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Yen-Li Li ◽  
Viswanathan Chandrasekaran ◽  
Stephen D Carter ◽  
Cora L Woodward ◽  
Devin E Christensen ◽  
...  
Keyword(s):  
Pattern Recognition ◽  
Reverse Transcription ◽  
Cyclophilin A ◽  
Restriction Factors ◽  
Viral Capsids ◽  
Ring Domains ◽  
Conformational Plasticity ◽  
Retroviral Infections ◽  
Hiv 1 ◽  
Terminal Domains

TRIM5 proteins are restriction factors that block retroviral infections by binding viral capsids and preventing reverse transcription. Capsid recognition is mediated by C-terminal domains on TRIM5α (SPRY) or TRIMCyp (cyclophilin A), which interact weakly with capsids. Efficient capsid recognition also requires the conserved N-terminal tripartite motifs (TRIM), which mediate oligomerization and create avidity effects. To characterize how TRIM5 proteins recognize viral capsids, we developed methods for isolating native recombinant TRIM5 proteins and purifying stable HIV-1 capsids. Biochemical and EM analyses revealed that TRIM5 proteins assembled into hexagonal nets, both alone and on capsid surfaces. These nets comprised open hexameric rings, with the SPRY domains centered on the edges and the B-box and RING domains at the vertices. Thus, the principles of hexagonal TRIM5 assembly and capsid pattern recognition are conserved across primates, allowing TRIM5 assemblies to maintain the conformational plasticity necessary to recognize divergent and pleomorphic retroviral capsids.

scholarly journals Identification of clinically approved small molecules that inhibit growth and affect transcript levels of developmentally regulated genes in the African trypanosome

2020 ◽  
Vol 14 (3) ◽  
pp. e0007790 ◽  
Author(s):  
Madison Elle Walsh ◽  
Eleanor Mary Naudzius ◽  
Savanah Jessica Diaz ◽  
Theodore William Wismar ◽  
Mikhail Martchenko Shilman ◽  
...  
Keyword(s):  
Small Molecules ◽  
Developmentally Regulated ◽  
Transcript Levels ◽  
African Trypanosome

Developmentally regulated cytokeratin gene in Xenopus laevis

1985 ◽  
Vol 5 (10) ◽  
pp. 2575-2581
Author(s):  
J A Winkles ◽  
T D Sargent ◽  
D A Parry ◽  
E Jonas ◽  
I B Dawid
Keyword(s):  
Amino Acid ◽  
Xenopus Laevis ◽  
Intermediate Filament ◽  
Type I ◽  
Developmentally Regulated ◽  
Amino Acid Homology ◽  
Intermediate Filament Proteins ◽  
Functional Roles ◽  
Terminal Domain ◽  
Terminal Domains

We have determined the sequence of cloned cDNAs derived from a 1,665-nucleotide mRNA which transiently accumulates during Xenopus laevis embryogenesis. Computer analysis of the deduced amino acid sequence revealed that this mRNA encodes a 47-kilodalton type I intermediate filament subunit, i.e., a cytokeratin. As is common to all intermediate filament subunits so far examined, the predicted polypeptide, named XK70, contains N- and C-terminal domains flanking a central alpha-helical rod domain. The overall amino acid homology between XK70 and a human 50-kilodalton type I keratin is 47%; homology within the alpha-helical domain is 57%. The N-terminal domain, which is not completely contained in our cDNAs, is basic, contains 42% serine plus alanine, and includes five copies of a six-amino-acid repeating unit. The C-terminal domain has a high alpha-helical content and contains a region with sequence homology to the C-terminal domains of other type I and type III intermediate filament proteins. We suggest that different keratin filament subtypes may have different functional roles during amphibian oogenesis and embryogenesis.

Einzelnukleotid-Polymorphismen in der Promotorregion von Cyclophilin A haben keinen EInfluss auf den natürlichen Verlauf einer Hepatitis C Virusinfektion

2013 ◽  
Vol 51 (01) ◽  
Author(s):  
T von Hahn ◽  
B Karavul ◽  
E Steinmann ◽  
A Potthoff ◽  
CP Strassburg ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Cyclophilin A

Identification of Differentially Expressed Genes Using cDNA-AFLP During Six Days In Vitro Tuberization of Potato

Zuriat ◽  
2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Nono Carsono ◽  
Christian Bachem
Keyword(s):  
Gene Expression ◽  
Developmental Process ◽  
Expression Patterns ◽  
Induction Medium ◽  
In Vitro Tuberization ◽  
Storage Organ ◽  
Developmentally Regulated ◽  
Study Gene Expression ◽  
Differential Pattern

Tuberization in potato is a complex developmental process resulting in the differentiation of stolon into the storage organ, tuber. During tuberization, change in gene expression has been known to occur. To study gene expression during tuberization over the time, in vitro tuberization system provides a suitable tool, due to its synchronous in tuber formation. An early six days axillary bud growing on tuber induction medium is a crucial development since a large number of genes change in their expression patterns during this period. In order to identify, isolate and sequencing the genes which displaying differential pattern between tuberizing and non-tuberizing potato explants during six days in vitro tuberization, cDNA-AFLP fingerprint, method for the visualization of gene expression using cDNA as template which is amplified to generate an RNA-fingerprinting, was used in this experiment. Seventeen primer combinations were chosen based on their expression profile from cDNA-AFLP fingerprint. Forty five TDFs (transcript derived fragment), which displayed differential expressions, were obtained. Tuberizing explants had much more TDFs, which developmentally regulated, than those from non tuberizing explants. Seven TDFs were isolated, cloned and then sequenced. One TDF did not find similarity in the current databases. The nucleotide sequence of TDF F showed best similarity to invertase ezymes from the databases. The homology of six TDFs with known sequences is discussed in this paper.

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