Relationship of seminal traits and insemination time to fertilization rate and embryo quality

Summary To determine the effects of sperm DNA fragmentation (SDF) on embryo morphokinetic parameters, cleavage patterns and embryo quality, this retrospective study analyzed 151 intracytoplasmic sperm injection (ICSI) cycles (1152 embryos collected) between November 2016 and June 2019. SDF was assessed using sperm chromatin dispersion. The cycles were divided into two groups based on the SDF rate: SDF < 15% (n = 114) and SDF ≥ 15% (n = 37). The embryo morphokinetic parameters, cleavage patterns, and embryo quality were compared between the two groups. The morphokinetic parameters tPNf, t2, t3, t4, t5, t6, and t8 were achieved significantly earlier in the SDF < 15% group compared with in the SDF ≥ 15% group. The fertilization and 2PN rates seemed to be significantly higher in the SDF < 15% group compared with in the SDF ≥ 15% group, while the abnormal cleavage rates were similar. However, a significantly higher rate of chaotic cleavage (CC) was observed in the SDF ≥ 15% group. The D3 high-quality embryo and available embryo rates were similar between the two groups. The blastocyst formation, high-quality blastocyst, and available blastocyst rates in the SDF < 15% group were significantly higher than those in the SDF ≥ 15% group. With an increase in SDF level, the chemical pregnancy, clinical pregnancy and implantation rates tended to decrease, while the miscarriage rate increased. This study demonstrated that SDF ≥ 15% reduces the fertilization rate of ICSI cycles and affects certain morphokinetic parameters. A higher SDF level can also induce a higher rate of CC, with subsequent decreases in the blastocyst formation rate and blastocyst quality.

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Prediction of fertilization rate and embryo quality based on oocyte morphological characteristics

Fertility and Sterility ◽

10.1016/j.fertnstert.2009.07.1505 ◽

2009 ◽

Vol 92 (3) ◽

pp. S216

Author(s):

M.D. Salazar ◽

P. Diaz ◽

G. Garcia ◽

J. Sepulveda ◽

R. Santos ◽

...

Keyword(s):

Embryo Quality ◽

Morphological Characteristics ◽

Fertilization Rate

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PR0P0F0L VS. THIOPENTAL NA

The Professional Medical Journal ◽

10.29309/tpmj/2009.16.04.2741 ◽

2009 ◽

Vol 16 (04) ◽

pp. 542-549

Author(s):

FARNOUSH FARZI ◽

Mona Oudi ◽

MARZIEH MEHRAFZA ◽

Zahra Mohammad Tabar ◽

ALI MIRMANSOURI ◽

...

Keyword(s):

Pregnancy Rate ◽

Embryo Quality ◽

Postoperative Nausea And Vomiting ◽

Oocyte Retrieval ◽

Good Alternative ◽

Fertilization Rate ◽

Nausea And Vomiting ◽

Informed Consent Form ◽

The Mean ◽

Post Operative Nausea

O b j e c t i v e : The aim of this study was to compare the side effects, fertilization rate and pregnancy rate (PR) and duration ofrecovery between Propofol and Thiopental Na after ICSI-vaginal retrieval of oocyte in ART cycle. D e s i g n : This study was a prospective,randomized clinical trial Materials and M e t h o d s : Ninety eight ASA(American Society of Anesthesiologist) physical status I and II womenparticipating in an intracytoplasmic sperm injection) ICSI) program were assessed. All of the patients underwent general anesthesia inductionwith Propofol and Thiopental Na. The first group (49cycles) received 2-2.5mg/kg of Propofol, and the second group (49cycles) received 5mg/kgThiopental during transvaginal oocyte retrieval. An informed consent form was obtained for each patients treatment. Variables under studyincluded: female age, cause and duration of infertiIity^postoperative nausea and vomiting(PONV), heamodynamic changes, mean number ofoocyte retrieved, oocyte metaphase II, embryo cleaved, embryo transferred, embryo quality and pregnancy rate(PR)and duration recovery.Statistical analysis was carried out by using SPSS.10 software and statistical test of T-test and chi-square. R e s u l t s : The PR in Propofol groupwas 18(36.7%) and in Thiopental Na group was 19(38.8%) with no significant differences the mean duration of infertility and weight weren'tstatistically significant. The mean number of oocyte retrieved (metaphase II), embryo cleaved, embryo transferred and embryo quality weren'tsignificant between the two groups. The incidence of nausea in Propofol group in comparison with Thiopental Na group was lower withsignificant differences. The incidence of vomiting between two groups was statistically significant (46.9% vs.28.6% respectively)(P<0.05).between two groups. Duration of recovery in Propofol group was 15+/_3min and in Thiopental Na group was 25+/_5 min that was statisticallysignificant(P<0.05). C o n c l u s i o n s : Propofol offered lower incidence of post operative nausea and vomiting and a quick recovery from anesthesia without any adverse effect on pregnancy outcome. These findings showed that Propofol was a good alternative for Thiopental Na in short timeoperation, like ICSI -vaginal retrieval of oocytes.

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Cytoplasmic Transfer Improves Human Egg Fertilization and Embryo Quality: an Evaluation of Sibling Oocytes in Women with Low Oocyte Quality

Reproductive Sciences ◽

10.1007/s43032-020-00371-8 ◽

2020 ◽

Author(s):

Ales Sobek ◽

Emil Tkadlec ◽

Eva Klaskova ◽

Martin Prochazka

Keyword(s):

Embryo Quality ◽

Oocyte Quality ◽

Fertilization Rate ◽

Early Embryo ◽

Vitro Fertilization ◽

Sibling Oocytes ◽

Fertilization Rates ◽

Group B ◽

Cytoplasmic Transfer

Abstract The aim of this study was to evaluate if cytoplasmic transfer can improve fertilization and embryo quality of women with oocytes of low quality. During ICSI, 10–15% of the cytoplasm from a fresh or frozen young donor oocyte was added to the recipient oocyte. According to the embryo quality, we defined group A as patients in which the best embryo was evident after cytoplasmic transfer and group B as patients in which the best embryo was evident after a simple ICSI. We investigated in the period of 2002–2018, 125 in vitro fertilization cycles involving 1011 fertilized oocytes. Five hundred fifty-seven sibling oocytes were fertilized using ICSI only and 454 oocytes with cytoplasmic transfer. Fertilization rates of oocytes were 67.2% in the cytoplasmic transfer and 53.5% in the ICSI groups (P < 0.001). A reduction in fertilization rate was observed with increased women age in the ICSI but not in the cytoplasmic transfer groups. The best embryo quality was found after cytoplasmic transfer in 78 cycles (62.4%) and without cytoplasmic transfer in 40 cycles (32%, P < 0.001). No significant differences were detected between the age, hormonal levels, dose of stimulation drugs, number of transferred embryos, pregnancy rate and abortion rate between A and B groups. Cytoplasmic transfer improves fertilization rates and early embryo development in humans with low oocyte quality. All 28 children resulting from cytoplasmic transfer are healthy.

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Prospective Randomized Study on the Influence of Myoinositol in PCOS Women Undergoing IVF in the Improvement of Oocyte Quality, Fertilization Rate, and Embryo Quality

International Journal of Endocrinology ◽

10.1155/2016/4378507 ◽

2016 ◽

Vol 2016 ◽

pp. 1-5 ◽

Cited By ~ 8

Author(s):

Bernd Lesoine ◽

Pedro-Antonio Regidor

Keyword(s):

Placebo Group ◽

Folic Acid ◽

Embryo Quality ◽

Randomized Study ◽

Oocyte Quality ◽

Fertilization Rate ◽

Clear Trend ◽

Fertilization Rates ◽

Group B

Polycystic ovarian syndrome (PCOS) is one of the pathological factors involved in the failure of in vitro fertilization (IvF). The aim of the present study was to investigate if the combination of myoinositol + folic acid was able to improve the oocyte quality, the ratio between follicles and retrieved oocytes, the fertilization rate, and the embryo quality in PCOS patients undergoing IvF treatments. 29 patients with PCOS underwent IvF protocols for infertility treatment and were randomized prospectively into two groups. Group A (placebo) with 15 patients and group B (4000 mg myoinositol + 400 μg folic acid per day) with 14 patients. The patients of group B used for two months myoinositol + folic acid before starting the IvF protocol and data were obtained concerning number of follicles, number of oocytes, quality of oocytes, fertilization rates, and embryo quality in both groups. The ratio follicle/retrieved oocyte was better in the myoinositol group (= group B). Out of the 233 oocytes collected in the myoinositol group 136 were fertilized, whereas only 128 out of 300 oocytes in the placebo group were fertilized. More metaphase II and I oocytes were retrieved in relation to the total amount of oocytes in the myoinositol. More embryos of grade I quality were obtained in the myoinositol. The duration of stimulation was 9,7 days (±3,3) in the myoinositol group and 11,2 (±1,8) days in the placebo group and the number of used FSH units was lower in the myoinositol group: 1750 FSH units (mean) versus 1850 units (mean). Our evidence suggests that myoinositol therapy in women with PCOS results in better fertilization rates and a clear trend to a better embryo quality. As the number of retrieved oocytes was smaller in the myoinositol group, the risk of hyper stimulation syndrome can be reduced in these patients.

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Towards the enhancement of pregnancy rate: The effect of insemination time on sperm transport, fertilization rate and embryo quality in dairy cattle

BSAP Occasional Publication ◽

10.1017/s0263967x00033668 ◽

2001 ◽

Vol 26 (1) ◽

pp. 161-174 ◽

Cited By ~ 1

Author(s):

J.C. Dalton ◽

S. Nadir ◽

J. Bame ◽

M. Noftsinger ◽

R.G. Saacke

Keyword(s):

Dairy Cattle ◽

Pregnancy Rate ◽

Embryo Quality ◽

Fertilization Rate ◽

Pregnancy Rates ◽

Sperm Number ◽

Sperm Transport ◽

Fertilization Rates ◽

Natural Service

AbstractTo further identify factors which influence pregnancy rates, three experiments were conducted to determine the effect of insemination time on sperm transport, fertilization rate, and embryo quality. All cows were continuously monitored for behavioural oestrus by HeatWatch®, and received AI at heat onset (0 h after the first standing event), 12 h after onset, or received natural service at 0 hfrom one of three bulls (Exp. 1). In Exp. 2, cows received AI at 0 h, 12 h, or 24 h after the first standing event. On d 6 after insemination 115 embryos(ova) (Exp. 1) and 117 embryos(ova) (Exp. 2) were recovered from single-ovulating cows. For Exp. 1, median accessory sperm values were: 1 (0 h), 10 (12 h), 27 (natural service O h) (P < 0.05). For Exp. 2, median accessory sperm values were: 1 (0 h), 2 (12 h), 4 (24 h) (P < 0.05). Fertilization rates were: 67% (0 h), 79% (12 h), 98% (natural service O h) (P < 0.05)(Exp. 1); and did not differ in Exp. 2. Embryo quality was not different in Exp. 1. In Exp. 2, percentages of excellent and good fair and poor, and degenerate embryos were: 77, 15, 8 (0 h), 52, 38, 10 (12 h), 47, 19, 34 (24 h) (P < 0.05). In Exp. 3, 30 cows were superovulated and were inseminated once at either 0 h, 12 h, or 24 h after the first standing event. On d 6 after insemination, 529 embryos(ova) were recovered. Fertilization rates were: 29% (0 h); 60% (12 h); 81% (24 h)(P < 0.01). Percentages of embryos with accessory sperm were: 5 (0 h); 8 (12 h); and 41(24 h) (P < 0.01). Embryo quality was not affected by time of AI. We conclude that the time of insemination affects: 1) sperm transport as measured by median accessory sperm number (Exp. 1 and 2) and the percentage of embryos with accessory sperm (Exp. 3); 2) fertilization rate (Exp. 1 and 3); and embryo quality (Exp. 2).

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