Towards the enhancement of pregnancy rate: The effect of insemination time on sperm transport, fertilization rate and embryo quality in dairy cattle

2001 ◽  
Vol 26 (1) ◽  
pp. 161-174 ◽  
Author(s):  
J.C. Dalton ◽  
S. Nadir ◽  
J. Bame ◽  
M. Noftsinger ◽  
R.G. Saacke
Keyword(s):  
Dairy Cattle ◽  
Pregnancy Rate ◽  
Embryo Quality ◽  
Fertilization Rate ◽  
Pregnancy Rates ◽  
Sperm Number ◽  
Sperm Transport ◽  
Fertilization Rates ◽  
Natural Service

AbstractTo further identify factors which influence pregnancy rates, three experiments were conducted to determine the effect of insemination time on sperm transport, fertilization rate, and embryo quality. All cows were continuously monitored for behavioural oestrus by HeatWatch®, and received AI at heat onset (0 h after the first standing event), 12 h after onset, or received natural service at 0 hfrom one of three bulls (Exp. 1). In Exp. 2, cows received AI at 0 h, 12 h, or 24 h after the first standing event. On d 6 after insemination 115 embryos(ova) (Exp. 1) and 117 embryos(ova) (Exp. 2) were recovered from single-ovulating cows. For Exp. 1, median accessory sperm values were: 1 (0 h), 10 (12 h), 27 (natural service O h) (P < 0.05). For Exp. 2, median accessory sperm values were: 1 (0 h), 2 (12 h), 4 (24 h) (P < 0.05). Fertilization rates were: 67% (0 h), 79% (12 h), 98% (natural service O h) (P < 0.05)(Exp. 1); and did not differ in Exp. 2. Embryo quality was not different in Exp. 1. In Exp. 2, percentages of excellent and good fair and poor, and degenerate embryos were: 77, 15, 8 (0 h), 52, 38, 10 (12 h), 47, 19, 34 (24 h) (P < 0.05). In Exp. 3, 30 cows were superovulated and were inseminated once at either 0 h, 12 h, or 24 h after the first standing event. On d 6 after insemination, 529 embryos(ova) were recovered. Fertilization rates were: 29% (0 h); 60% (12 h); 81% (24 h)(P < 0.01). Percentages of embryos with accessory sperm were: 5 (0 h); 8 (12 h); and 41(24 h) (P < 0.01). Embryo quality was not affected by time of AI. We conclude that the time of insemination affects: 1) sperm transport as measured by median accessory sperm number (Exp. 1 and 2) and the percentage of embryos with accessory sperm (Exp. 3); 2) fertilization rate (Exp. 1 and 3); and embryo quality (Exp. 2).

scholarly journals Impact of Living in Different Altitudes on Intracytoplasmic Sperm Injection (ICSI) Outcomes for Infertile Couples in Southwestern Region of Saudi Arabia, a Retrospective Study

2022 ◽  
Vol 6 (1) ◽  
pp. 01-07
Author(s):  
Mamdoh Eskandar ◽  
Wardah Alasmari ◽  
Fawaz Idris ◽  
Huda Nadwi ◽  
Enshrah Radwan ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Retrospective Study ◽  
Pregnancy Rate ◽  
Embryo Quality ◽  
Pregnancy Rates ◽  
Clinical Pregnancy ◽  
Fertilization Rates ◽  
Infertile Couples ◽  
Icsi Outcomes ◽  
Different Altitudes

Objective: The aim of this study was to determine the effect of different altitudes in the Southwestern region of Saudi Arabia on ICSI outcomes, fertilization rate, embryo quality, pregnancy rate, and miscarriage rates for infertile couples. Materials and Methods: This is a retrospective study on 551 infertile couples carried out in the Assisted Reproductive Technology unit at the Maternity and Childern Hospital in Abha, Saudi Arabia between 2018 and 2019 to compare ICSI outcomes in different altitudes. Low altitude (205 patients), mild altitude (86 patients) and high altitude (260 patients). Main result measurements: fertilization rates, embryo quality, clinical pregnancy and miscarriage rates at different altitudes. Results: The data showed that there were no significant differences (P>0.67) in fertilization rates and the number of good-quality embryos between different altitudes. Importantly, clinical pregnancy rates were similar between groups and there was no significant difference in the miscarriage rates between high, mild and low altitudes. Conclusions: This study demonstrates that there is now increased risk of miscarriage or low pregnancy rates with different altitudes in Southwestern region of Saudi Arabia. This suggests that altitude changes has no obvious risk on pregnancy rate and pregnancy outcome.

scholarly journals PR0P0F0L VS. THIOPENTAL NA

2009 ◽  
Vol 16 (04) ◽  
pp. 542-549
Author(s):  
FARNOUSH FARZI ◽  
Mona Oudi ◽  
MARZIEH MEHRAFZA ◽  
Zahra Mohammad Tabar ◽  
ALI MIRMANSOURI ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Embryo Quality ◽  
Postoperative Nausea And Vomiting ◽  
Oocyte Retrieval ◽  
Good Alternative ◽  
Fertilization Rate ◽  
Nausea And Vomiting ◽  
Informed Consent Form ◽  
The Mean ◽  
Post Operative Nausea

O b j e c t i v e : The aim of this study was to compare the side effects, fertilization rate and pregnancy rate (PR) and duration ofrecovery between Propofol and Thiopental Na after ICSI-vaginal retrieval of oocyte in ART cycle. D e s i g n : This study was a prospective,randomized clinical trial Materials and M e t h o d s : Ninety eight ASA(American Society of Anesthesiologist) physical status I and II womenparticipating in an intracytoplasmic sperm injection) ICSI) program were assessed. All of the patients underwent general anesthesia inductionwith Propofol and Thiopental Na. The first group (49cycles) received 2-2.5mg/kg of Propofol, and the second group (49cycles) received 5mg/kgThiopental during transvaginal oocyte retrieval. An informed consent form was obtained for each patients treatment. Variables under studyincluded: female age, cause and duration of infertiIity^postoperative nausea and vomiting(PONV), heamodynamic changes, mean number ofoocyte retrieved, oocyte metaphase II, embryo cleaved, embryo transferred, embryo quality and pregnancy rate(PR)and duration recovery.Statistical analysis was carried out by using SPSS.10 software and statistical test of T-test and chi-square. R e s u l t s : The PR in Propofol groupwas 18(36.7%) and in Thiopental Na group was 19(38.8%) with no significant differences the mean duration of infertility and weight weren'tstatistically significant. The mean number of oocyte retrieved (metaphase II), embryo cleaved, embryo transferred and embryo quality weren'tsignificant between the two groups. The incidence of nausea in Propofol group in comparison with Thiopental Na group was lower withsignificant differences. The incidence of vomiting between two groups was statistically significant (46.9% vs.28.6% respectively)(P<0.05).between two groups. Duration of recovery in Propofol group was 15+/_3min and in Thiopental Na group was 25+/_5 min that was statisticallysignificant(P<0.05). C o n c l u s i o n s : Propofol offered lower incidence of post operative nausea and vomiting and a quick recovery from anesthesia without any adverse effect on pregnancy outcome. These findings showed that Propofol was a good alternative for Thiopental Na in short timeoperation, like ICSI -vaginal retrieval of oocytes.

P–708 Comparison of reproductive outcomes after progestin-primed ovarian stimulation with dydrogesterone versus cetrorelix to inhibit spontaneous ovulation in oocyte donation

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
J A Moreno ◽  
P Masoli ◽  
C Sferrazza ◽  
H Leiva ◽  
O Espinosa ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Live Birth ◽  
Ovarian Stimulation ◽  
Fertilization Rate ◽  
Oocyte Donation ◽  
Pregnancy Rates ◽  
Clinical Pregnancy ◽  
Reproductive Outcomes ◽  
Ongoing Pregnancy ◽  
Live Births

Abstract Study question Is dydrogesterone (DYG) equivalent compared to cetrorelix with respect to clinical pregnancy rate, ongoing pregnancy rate and live birth rate in oocyte donation (OD) cycles? Summary answer DYG is comparable to cetrorelix in terms of clinical pregnancy, but higher rates of ongoing pregnancy and live birth were observed in the DYG group What is known already Progestin-primed ovarian stimulation (PPOS) is an ovarian stimulation regimen based on a freeze-all strategy using progestin as an alternative to GnRH analog for suppressing a premature LH surge. DYG is an oral progestin that has been studied in PPOS protocols. Published reports indicate that length of ovarian stimulation, dose of gonadotrophin needed and number of MII retrieved from PPOS cycles are comparable to short protocol of GnRH agonists during OD cycles. However, while some studies noted no differences in terms of live births, worse pregnancy rates have been reported in recipients of oocytes from PPOS cycles compared to GnRH antagonists. Study design, size, duration Prospective controlled study to assess the reproductive outcomes of OD recipients in which the donors were subjected to the DYG protocol (20mg/day) compared with those subjected to the short protocol with cetrorelix (0.25 mg/day) from Day 7 or since a leading follicle reached 14 mm. The OD cycles were triggered with triptoreline acetate and the trigger criterion was ≥3 follicles of diameter &gt;18mm. Participants/materials, setting, methods 202 oocyte donors were included, 92 under DYG and 110 under cetrorelix. The study was performed in a private infertility center between January 2017 and December 2020. The main outcome included the rates of clinical pregnancy, ongoing pregnancy and live births. Secondary outcomes included the number of oocytes retrieved, number of MII, fertilization rate, length of stimulation and total gonadotropin dose. Differences were tested using a Student’s t-test or a Chi2 test, as appropriate. Main results and the role of chance Compared to antagonist cycles, cycles under DYG had fewer days of stimulation (9.9 ± 0.9 vs. 10.8 ± 1.1, p&lt;.001) and a lower total gonadotropin dose (1654 ± 402.4 IU vs. 1844 ± 422 IU, p&lt;.001). The number of MII retrieved was no different: 16.9 (SD 6.2) with DYG and 15.4 (SD 5.8) with cetrorelix (p = 0.072). Recipients and embryo transfer (ET) characteristics were also similar between groups. The mean number of MII assigned to each recipients was 6.7 (SD 1.8) in DYG and 6.6 (SD 1.7) in cetrorelix (P = 0.446). The fertilization rate was 66.2% in DYG versus 67.6% in cetrorelix (P = 0.68). Regarding the reproductive outcomes, the overall clinical pregnancy rate in DYG group (65/87: 74.7%) and cetrorelix group (66/104: 63.4%) (p = 0.118) was similar. Meanwhile, the DYG group compared to cetrorelix group had higher rates of ongoing pregnancy (63.2% vs 45.1%; p = 0.014) and live births (54,9% vs 37.8%; p = 0.040). Limitations, reasons for caution These results should be evaluated with caution. The limitations of this study include the limited number of participants enrolled and the limited data on pregnancy outcomes. A randomized controlled trial is necessary to provide more evidence on the efficacy of the DYG protocol. Wider implications of the findings: The efficacy of PPOS protocol compared to GnRH-antagonist protocol in terms of reproductive outcomes has been little studied. PPOS using DYG yields comparable clinical pregnancy rates compared to cetrorelix in OD cycles. The differences found regarding the rates of ongoing pregnancy and live births should be further investigated. Trial registration number Not applicable

scholarly journals Cytoplasmic Transfer Improves Human Egg Fertilization and Embryo Quality: an Evaluation of Sibling Oocytes in Women with Low Oocyte Quality

2020 ◽  
Author(s):  
Ales Sobek ◽  
Emil Tkadlec ◽  
Eva Klaskova ◽  
Martin Prochazka
Keyword(s):  
Embryo Quality ◽  
Oocyte Quality ◽  
Fertilization Rate ◽  
Early Embryo ◽  
Vitro Fertilization ◽  
Sibling Oocytes ◽  
Fertilization Rates ◽  
Group B ◽  
Cytoplasmic Transfer

Abstract The aim of this study was to evaluate if cytoplasmic transfer can improve fertilization and embryo quality of women with oocytes of low quality. During ICSI, 10–15% of the cytoplasm from a fresh or frozen young donor oocyte was added to the recipient oocyte. According to the embryo quality, we defined group A as patients in which the best embryo was evident after cytoplasmic transfer and group B as patients in which the best embryo was evident after a simple ICSI. We investigated in the period of 2002–2018, 125 in vitro fertilization cycles involving 1011 fertilized oocytes. Five hundred fifty-seven sibling oocytes were fertilized using ICSI only and 454 oocytes with cytoplasmic transfer. Fertilization rates of oocytes were 67.2% in the cytoplasmic transfer and 53.5% in the ICSI groups (P < 0.001). A reduction in fertilization rate was observed with increased women age in the ICSI but not in the cytoplasmic transfer groups. The best embryo quality was found after cytoplasmic transfer in 78 cycles (62.4%) and without cytoplasmic transfer in 40 cycles (32%, P < 0.001). No significant differences were detected between the age, hormonal levels, dose of stimulation drugs, number of transferred embryos, pregnancy rate and abortion rate between A and B groups. Cytoplasmic transfer improves fertilization rates and early embryo development in humans with low oocyte quality. All 28 children resulting from cytoplasmic transfer are healthy.

scholarly journals Prospective Randomized Study on the Influence of Myoinositol in PCOS Women Undergoing IVF in the Improvement of Oocyte Quality, Fertilization Rate, and Embryo Quality

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Bernd Lesoine ◽  
Pedro-Antonio Regidor
Keyword(s):  
Placebo Group ◽  
Folic Acid ◽  
Embryo Quality ◽  
Randomized Study ◽  
Oocyte Quality ◽  
Fertilization Rate ◽  
Clear Trend ◽  
Fertilization Rates ◽  
Group B

Polycystic ovarian syndrome (PCOS) is one of the pathological factors involved in the failure of in vitro fertilization (IvF). The aim of the present study was to investigate if the combination of myoinositol + folic acid was able to improve the oocyte quality, the ratio between follicles and retrieved oocytes, the fertilization rate, and the embryo quality in PCOS patients undergoing IvF treatments. 29 patients with PCOS underwent IvF protocols for infertility treatment and were randomized prospectively into two groups. Group A (placebo) with 15 patients and group B (4000 mg myoinositol + 400 μg folic acid per day) with 14 patients. The patients of group B used for two months myoinositol + folic acid before starting the IvF protocol and data were obtained concerning number of follicles, number of oocytes, quality of oocytes, fertilization rates, and embryo quality in both groups. The ratio follicle/retrieved oocyte was better in the myoinositol group (= group B). Out of the 233 oocytes collected in the myoinositol group 136 were fertilized, whereas only 128 out of 300 oocytes in the placebo group were fertilized. More metaphase II and I oocytes were retrieved in relation to the total amount of oocytes in the myoinositol. More embryos of grade I quality were obtained in the myoinositol. The duration of stimulation was 9,7 days (±3,3) in the myoinositol group and 11,2 (±1,8) days in the placebo group and the number of used FSH units was lower in the myoinositol group: 1750 FSH units (mean) versus 1850 units (mean). Our evidence suggests that myoinositol therapy in women with PCOS results in better fertilization rates and a clear trend to a better embryo quality. As the number of retrieved oocytes was smaller in the myoinositol group, the risk of hyper stimulation syndrome can be reduced in these patients.

scholarly journals 181COMPARISON OF THE PREGNANCY RATES AFTER SYNCHRONIZATION OF OVULATION USING GnRH AND PGF2± IN RECIPIENT DAIRY CATTLE

2004 ◽  
Vol 16 (2) ◽  
pp. 212 ◽  
Author(s):  
T. Nishisouzu ◽  
M. Sugawara ◽  
S. Aoki ◽  
K. Kishida ◽  
M. Moriyoshi ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Control Group ◽  
Corpora Lutea ◽  
Transfer Program ◽  
Chi Square ◽  
Follicle Diameter

Treatments with GnRH and PGF2α for synchronization of ovulation has resulted in acceptable pregnancy rates after fixed-time artificial insemination in dairy cows without estrus detection. The objective of the present study was to evaluate the practicability of ovulation synchronization (Ovsynch, Pursley JR et al. 1995 Theriogenology 44, 915–923) in dairy cattle using GnRH and PGF2α for the embryo transfer recipients. Dairy cattle (cows; n=100, heifers; n=88) were randomly allocated to one of two groups. The control group (cows; n=45, heifers; n=37) was composed of cows in natural estrus. The ovulation synchronization group (cows; n=55, heifers; n=51) was treated with an intramuscular injection of 100μg of GnRH at a random stage of the estrous cycle. Seven days later, the cattle received PGF2α (Cows; 25–30mg) or PGF2α analog (Heifers; 0.5mg) in order to regress the corpora lutea (CL). Forty-eight hours later, cows and heifers received a second injection of 100μg GnRH. Embryo transfer was carried out 7 days after the second injection of GnRH in the ovsynch group and 7 days after estrus in the control group. The cattle judged to have CL 17mm were classified as acceptable recipients. The size of the follicles and the CL were determined to be of estrus stage and embryo transfer by means of ultrasonography. The mean numbers of follicles and CL were analyzed by ANOVA, while pregnancy rates were analyzed by chi-square test. The results are presented in the Table. The proportion of cows and heifers determined to be acceptable embryo transfers was not different between the control group and the ovsynch group. There were no differences in the proportion of acceptable embryo transfers between the control group and the ovsynch group. Follicle diameter at the time of estrus in the control group (cows; 20.7±0.7mm, heifers; 16.8±0.5mm) were significantly larger than that of the ovsynch group (cows; 18.0±1.0mm, heifers; 14.7±0.2mm) (P&lt;0.05). Although CL diameter at the time of embryo transfer in heifers showed no differences between the control group and the ovsynch group (25.0±1.0mm v. 22.8±1.5mm), The CL diameter of the control cow group was larger than that of the ovsynch group (29.8±0.7mm v. 26.1±1.0mm, P&lt;0.05). However, no differences in pregnancy rate were seen between the control group and the ovsynch group. These results suggest that ovsynch can be effectively applied in an embryo transfer program for cattle. Table 1 Proportion of acceptable embryo transfer recipients and pregnancy rate in dairy cattle in the control ovsynch groups

216 THE RELATIONSHIP BETWEEN OXYGEN CONSUMPTION RATE AND PREGNANCY RATE OF BOVINE EMBRYOS

2007 ◽  
Vol 19 (1) ◽  
pp. 225 ◽  
Author(s):  
N. Sakagami ◽  
K. Akiyama ◽  
Y. Nakazawa
Keyword(s):  
Oxygen Consumption ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Embryo Quality ◽  
Calf Serum ◽  
Pregnancy Rates ◽  
Bovine Embryos ◽  
Significant Difference ◽  
Consumption Rates ◽  
The Relationship

A precise evaluation of embryo quality is important to estimate the suitability of embryo transfer to recipient animal. Recently, an objective evaluation method was reported for bovine embryos, in which the oxygen consumption of embryos can be noninvasively determined by scanning electrochemical microscopy (SECM) (Shiku et al. 2001 Anal. Chem. 73, 3751–3758). Trimarchi et al. (2000 Biol. Reprod. 62, 1866–1874) suggested that the oxygen consumption reflects the cell number and mitochondrial activity of embryos. The objectives of this study were (1) to examine the oxygen consumption of in vivo-derived embryos by SECM, (2) to investigate the relationship between oxygen consumption and morphological estimation of embryos, and (3) to assess the correlation among the oxygen consumption, embryo viability, and pregnancy rates. Fifty-six embryos were collected from Japanese Black cattle, which were superovulated with a total dose of 20 mg porcine FSH (FSH-R; Kawasaki Pharmaceutical Co., Ltd., Tokyo, Japan) followed by AI. The qualities of collected embryos at the stage of compacted morulae (CM), early blastocysts (EB), and blastocysts (BL) on Day 7 after AI were categorized as grade 1 and grade 2, according to the IETS manual (2002). The oxygen consumption rates of embryos were evaluated by SECM, as previously described by Abe et al. (2004 J. Mamm. Ova Res. 21). Embryos were frozen by programmable freezer in Dulbecco&apos;s PBS containing 1.5 M ethylene glycol, 0.1 M trehalose, and 20&percnt; calf serum. They were thawed by holding the straws in air for 8 s and then immersing them in a 30&deg;C water bath for 15 s. After thawing, the embryos were examined for oxygen consumption. Twenty-eight embryos were then cultured in TCM-199 supplemented with 20&percnt; fetal bovine serum and 0.1 mM &beta;-mercaptoethanol for 24 h to assess the viability of embryos by re-expansion of blastocole. The remaining 28 embryos were transferred to recipients. The pregnancy rates were determined by rectal palpation on Day 70. Data were analyzed by ANOVA. The consumption rates of BL embryos on Day 7 were significantly higher (P &lt; 0.05) than those of CM collected on the same day (0.84 vs. 1.29 &times; 10&minus;14 mol s&minus;1, respectively). A significant difference was also observed in consumption rates between grade 1 and 2 embryos at the BL stage (P &lt; 0.05). After freezing&ndash;thawing, the average oxygen consumption rates of embryos were 0.52 &times; 10&minus;14 mol s&minus;1 for CM (n &equals; 9), 0.67 &times; 10&minus;14 mol s&minus;1 for EB (n &equals; 8), and 0.96 &times; 10&minus;14 mol s&minus;1 for BL (n &equals; 11). The CM embryos with rates of &lt; 0.5 &times; 10&minus;14 mol s&minus;1 and the EB and BL embryos with those &lt; 0.6 &times; 10&minus;14 mol s&minus;1 did not show good morphological appearance after 24 h in culture. Pregnant animals were not obtained from embryos with rates &lt;0.5 &times; 10&minus;14 mol s&minus;1 for CM (n &equals; 5) and &lt;0.7 &times; 10&minus;14 mol s&minus;1 for EB (n &equals; 9). A high pregnancy rate (67&percnt;) was obtained from embryos with rates &gt;1.0 &times; 10&minus;14 mol s&minus;1 for BL (n &equals; 14). These results suggest that the measurement of oxygen consumption of embryos after embryo freezing and prior to embryo transfer may be useful for estimating embryo quality and suitability of embryo transfer.

The impact of seasonal variations on fertilization rate, embryo quality, and pregnancy rates during intracytoplasmic sperm injection

2003 ◽  
Vol 80 ◽  
pp. 170-171
Author(s):  
Ramazan Mercan ◽  
Alper Mumcu ◽  
Aycan Isiklar ◽  
Basak Balaban ◽  
Cengiz Alatas ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Seasonal Variations ◽  
Embryo Quality ◽  
Fertilization Rate ◽  
Pregnancy Rates ◽  
The Impact

scholarly journals The Effect of Vitrification of Oocytes Cumulus Complex Apoptosis of Mice (Mus musculus) to Apoptosis, Rate of Fertilization and Embryo Quality

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Widjiati Widjiati ◽  
Aulanni’am Aulanni’am ◽  
Viski Fitri Hendrawan
Keyword(s):  
Mus Musculus ◽  
Embryo Quality ◽  
Cold Shock ◽  
Molecular Level ◽  
Fertilization Rate ◽  
Apoptosis Rate ◽  
Fertilization Rates ◽  
Cumulus Oocyte Complex

The purpose of this study was to determine the quality of cumulus oocyte complex (COC) after vitrification process toward apotosis of COC, fertilization rate and embryo quality. Frozen occurs during the cold shock that can lead to changes in the molecular level COC. These changes will affect the quality of frozen thawed oocytes after COC. The study used two groups, There are COC not frozen and frozen COC. Parameter observed were apotosis of blastomere cells, fertilization rate and embryo quality. Apoptosis of COC were observed with tunnel apoptec staining, fertilization rates were measured based on number of zygotes and embryo quality were observed through number of blastocyst. The data of apotosis of blastomere cells were analyzed by Kruscal Wallis. The result showed that the apoptosis number, fertilization rate, and morula number between are significantly (p less than 0.05) between groups. The administration of frozen COC increase number of apoptotic blastomer cells, decreased fertilization rate up to 51.1% and embryo quality up to 69.2 %. In conclusion, Frozen on COC increased apotosis of COC, decreased both of fertilization rate and embryo quality. The embryo Frozen of vitrification is required in the ART technology necessary to add an antioxidant to improve the fertilization rate and embryo quality

Fertilization, sperm transport, and early embryonic loss in ewes grazed on cultivars of subterranean clover. (T. subterraneum)

1970 ◽  
Vol 21 (1) ◽  
pp. 107 ◽  
Author(s):  
cC Kaltenbach ◽  
HL Davies
Keyword(s):  
Subterranean Clover ◽  
Fertilization Rate ◽  
Reproductive Failure ◽  
Embryonic Survival ◽  
Sperm Transport ◽  
Fertilization Rates ◽  
Significant Difference ◽  
Embryonic Loss

The causes of infertility due to phyto-oestrogens were investigated in ewes which had grazed Dwalganup, Yarloop, Geraldton, Woogenellup, and Mount Barker cultivars of subterranean clover (T. subterraneum L.) for 5 years. At laparotomy, sperm transport was investigated by making counts of spermatozoa in the oviduct 22-26 hr post coitum; fertilization rates were checked 72 hr post coitum and embryonic survival at 27-31 days. A larger proportion of ewes on high oestrogen cultivars Dwalganup, Yarloop, and Geraldton) had low oviducal spermatozoa counts and the fertilization rate was significantly lower (P < 0.05) than in ewes grazed on low oestrogen cultivars (Woogenellup and Mount Barker) (44% v. 63 %). There was no statistically significant difference in embryonic survival between these groups. Merino ewes had fewer oviducal spermatozoa and a lower (P < 0.05) fertilization rate than Border Leicester x Merino ewes. Impaired sperm transport is considered to be the reason for reproductive failure in the ewes that had grazed the high oestrogen cultivars of subterranean clover.

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