Rare earth separations using cation-exchange column chromatography

1973 ◽  
Vol 79 ◽  
pp. 287-291 ◽  
Author(s):  
Paul J. Karol
Keyword(s):  
Rare Earth ◽  
Cation Exchange ◽  
Column Chromatography ◽  
Exchange Column ◽  
Cation Exchange Column

Lysozyme Separation from Egg White by Cation Exchange Column Chromatography

1986 ◽  
Vol 51 (4) ◽  
pp. 1032-1036 ◽  
Author(s):  
E. LI-CHAN ◽  
S. NAKAI ◽  
J. SIM ◽  
D.B. BRAGG ◽  
K.V. LO
Keyword(s):  
Cation Exchange ◽  
Column Chromatography ◽  
Egg White ◽  
Exchange Column ◽  
Cation Exchange Column

scholarly journals Structural similarity of chymopapain forms as indicated by circular dichroism

1989 ◽  
Vol 257 (1) ◽  
pp. 183-186 ◽  
Author(s):  
S Solis-Mendiola ◽  
R Zubillaga-Luna ◽  
A Rojo-Dominguez ◽  
A Hernandez-Arana
Keyword(s):  
Liquid Chromatography ◽  
High Resolution ◽  
Cation Exchange ◽  
Proteolytic Activity ◽  
Structural Similarity ◽  
Tertiary Structures ◽  
Exchange Column ◽  
Cation Exchange Column ◽  
Modified Proteins ◽  
Circular Dichroïsm

Four chymopapain forms were isolated by high-resolution liquid chromatography on a cation-exchange column. The three major forms possess nearly identical secondary and tertiary structures, as judged from their c.d. spectra; these components showed similar proteolytic activity and Mr values close to that of papain. The fourth isolated component seems to be a mixture of modified proteins.

Hemoglobin A1C by Hplc with the Pharmacia Mono S HR 5/N Cation-Exchange Column: Influence of Sample Protein Load on Optimal Chromatographic Conditions

1992 ◽  
Vol 38 (8) ◽  
pp. 1488-1490 ◽  
Author(s):  
J C Philcox ◽  
M R Haywood ◽  
A M Rofe
Keyword(s):  
Cation Exchange ◽  
Flow Rate ◽  
Chromatographic Separation ◽  
Hemoglobin A1c ◽  
Analysis Time ◽  
Exchange Column ◽  
Cation Exchange Column ◽  
Protein Load

Abstract The Pharmacia Mono S HR 5/5 column has been optimized for hemoglobin A1c analysis by HPLC by using a much smaller column load, decreased buffer flow rate, and a steeper gradient than was used in previously described methods. Superior chromatographic separation, shorter analysis time, and a greatly extended column life have resulted.

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