Lipopolysaccharide-induced expression of inducible nitric oxide synthase in the guinea pig organ of Corti

2000 ◽  
Vol 140 (1-2) ◽  
pp. 91-98 ◽  
Author(s):  
Masaya Takumida ◽  
Matti Anniko ◽  
Raul Popa ◽  
De Ming Zhang
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Guinea Pig ◽  
Inducible Nitric Oxide Synthase ◽  
Organ Of Corti ◽  
Induced Expression ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

High-risk human papillomavirus-induced expression of endothelial and inducible nitric oxide synthase in human uterine cervix

2012 ◽  
Vol 45 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Päivi Rahkola-Soisalo ◽  
Hanna Savolainen-Peltonen ◽  
Mervi Väisänen-Tommiska ◽  
Ralf Butzow ◽  
Olavi Ylikorkala ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Human Papillomavirus ◽  
High Risk ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Uterine Cervix ◽  
Induced Expression ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Molecular cloning and characterization of Ca2+-dependent inducible nitric oxide synthase from guinea-pig lung

1998 ◽  
Vol 333 (3) ◽  
pp. 795-799 ◽  
Author(s):  
Manabu SHIRATO ◽  
Tohru SAKAMOTO ◽  
Yoshiyuki UCHIDA ◽  
Akihiro NOMURA ◽  
Yukio ISHII ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Amino Acid ◽  
Nitric Oxide Synthase ◽  
Guinea Pig ◽  
Inducible Nitric Oxide Synthase ◽  
Inos Mrna ◽  
Dependent Manner ◽  
Calmodulin Antagonist ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

We have isolated a full-length cDNA for an inducible nitric oxide synthase (iNOS) from guinea-pig lung. The cDNA has a 3447 bp open reading frame encoding 1149 amino acid residues. The deduced amino acid sequence is approx. 80% identical with iNOS of human epithelial cells and murine macrophages. Consensus recognition sites for cofactors are highly conserved. COS cell lysate transfected with the guinea-pig iNOS shows significant levels of nitric oxide synthase (NOS) activity, and this is inhibited by 79% by chelation of Ca2+ ions. The NOS activity is restored in a concentration-dependent manner by increasing the free Ca2+ level. The NOS activity is also inhibited by trifluoperazine, a calmodulin antagonist, which suggests that the Ca2+ dependence is due to Ca2+-dependent calmodulin binding to the enzyme. Northern blot analysis reveals that the cloned iNOS mRNA is expressed in the lung and the colon in normal guinea pigs. Stimulation in vivo by lipopolysaccharide induces the expression of iNOS in the kidney, the spleen and the colon, but in the lung the same stimulation decreases its expression. These results suggest that the cloned guinea-pig iNOS is distinct in characteristics and expression from previously described iNOS forms.

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