Inhibitory effects of methanol extract of Cyperus rotundus rhizomes on nitric oxide and superoxide productions by murine macrophage cell line, RAW 264.7 cells

2001 ◽  
Vol 76 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Won-Gil Seo ◽  
Hyun-Ock Pae ◽  
Gi-Su Oh ◽  
Kyu-Yun Chai ◽  
Tae-Oh Kwon ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Line ◽  
Methanol Extract ◽  
Cyperus Rotundus ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Inhibitory Effects ◽  
Macrophage Cell

Ferritin does not donate its iron for haem synthesis in macrophages

2010 ◽  
Vol 429 (3) ◽  
pp. 463-471 ◽  
Author(s):  
Marc Mikhael ◽  
Alex D. Sheftel ◽  
Prem Ponka
Keyword(s):  
Single Cell ◽  
Cell Line ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Iron Storage ◽  
Subunit Protein ◽  
Metabolic Functions

Iron is essential for all life, yet can be dangerous under certain conditions. Iron storage by the 24-subunit protein ferritin renders excess amounts of the metal non-reactive and, consequentially, ferritin is crucial for life. Although the mechanism detailing the storage of iron in ferritin has been well characterized, little is known about the fate of ferritin-stored iron and whether it can be released and reutilized for metabolic use within a single cell. Virtually nothing is known about the use of ferritin-derived iron in non-erythroid cells. We therefore attempted to answer the question of whether iron from ferritin can be used for haem synthesis in the murine macrophage cell line RAW 264.7 cells. Cells treated with ALA (5-aminolaevulinic acid; a precursor of haem synthesis) show increased haem production as determined by enhanced incorporation of transferrin-bound 59Fe into haem. However, the present study shows that, upon the addition of ALA, 59Fe from ferritin cannot be incorporated into haem. Additionally, little 59Fe is liberated from ferritin when haem synthesis is increased upon addition of ALA. In conclusion, ferritin in cultivated macrophages is not a significant source of iron for the cell's own metabolic functions.

scholarly journals Evaluation of Methods for Transient Transfection of a Murine Macrophage Cell Line, RAW 264.7

BioTechniques ◽  
1999 ◽  
Vol 27 (4) ◽  
pp. 824-832 ◽  
Author(s):  
C.D. Thompson ◽  
M.R. Frazier-Jessen ◽  
R. Rawat ◽  
R.P. Nordan ◽  
R.T. Brown
Keyword(s):  
Cell Line ◽  
Transient Transfection ◽  
Raw 264.7 ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line ◽  
Evaluation Of Methods

Raman imaging of heme metabolism in situ in macrophages and Kupffer cells

The Analyst ◽  
2018 ◽  
Vol 143 (14) ◽  
pp. 3489-3498 ◽  
Author(s):  
J. Dybas ◽  
M. Grosicki ◽  
M. Baranska ◽  
K. M. Marzec
Keyword(s):  
Cell Line ◽  
Kupffer Cells ◽  
Blood Cells ◽  
Raman Imaging ◽  
Raw 264.7 ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Imaging Results

Herein, we provide the Raman imaging results for different stages of erythrophagocytosis of senescent red blood cells executed by isolated murine primary Kupffer cells and a murine macrophage cell line (RAW 264.7).

scholarly journals Down-Regulation of Glycosylphosphatidylinositol-Specific Phospholipase D Induced by Lipopolysaccharide and Oxidative Stress in the Murine Monocyte- Macrophage Cell Line RAW 264.7

2001 ◽  
Vol 69 (5) ◽  
pp. 3214-3223 ◽  
Author(s):  
Xiaohan Du ◽  
Martin G. Low
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Response ◽  
Cell Line ◽  
Phospholipase D ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Down Regulation ◽  
Tnf Α

ABSTRACT Serum glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) activity is reduced over 75% in systemic inflammatory response syndrome. To investigate the mechanism of this response, expression of the GPI-PLD gene was studied in the mouse monocyte-macrophage cell line RAW 264.7 stimulated with lipopolysaccharide (LPS; 0.5 to 50 ng/ml). GPI-PLD mRNA was reduced approximately 60% in a time- and dose-dependent manner. Oxidative stress induced by 0.5 mM H2O2 or 50 μM menadione also caused a greater than 50% reduction in GPI-PLD mRNA. The antioxidant N-acetyl-l-cysteine attenuated the down-regulatory effect of H2O2but not of LPS. Cotreatment of the cells with actinomycin D inhibited down-regulation induced by either LPS or H2O2. The half-life of GPI-PLD mRNA was not affected by LPS, or decreased slightly with H2O2, indicating that the reduction in GPI-PLD mRNA is due primarily to transcriptional regulation. Stimulation with tumor necrosis factor alpha (TNF-α) resulted in ∼40% reduction in GPI-PLD mRNA in human A549 alveolar carcinoma cells but not RAW 264.7 cells, suggesting that alternative pathways could exist in different cell types for down-regulating GPI-PLD expression during an inflammatory response and the TNF-α autocrine signaling mechanism alone is not sufficient to recapitulate the LPS-induced reduction of GPI-PLD in macrophages. Sublines of RAW 264.7 cells with reduced GPI-PLD expression exhibited increased cell sensitivity to LPS stimulation and membrane-anchored CD14 expression on the cell surface. Our data suggest that down-regulation of GPI-PLD could play an important role in the control of proinflammatory responses.

Sesquiterpenes from Inula japonica with Inhibitory Effects on Nitric Oxide Production in Murine Macrophage RAW 264.7 Cells

2016 ◽  
Vol 79 (6) ◽  
pp. 1548-1553 ◽  
Author(s):  
Qinghao Jin ◽  
Jin Woo Lee ◽  
Hari Jang ◽  
Ji Eun Choi ◽  
Dongho Lee ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Murine Macrophage ◽  
Inhibitory Effects ◽  
Oxide Production

Inhibitory effects of Irigenin from the rhizomes of Belamcanda chinensis on nitric oxide and prostaglandin E2 production in murine macrophage RAW 264.7 cells

Life Sciences ◽  
2006 ◽  
Vol 78 (20) ◽  
pp. 2336-2342 ◽  
Author(s):  
Kwang Seok Ahn ◽  
Eun Jung Noh ◽  
Kwang-Hyun Cha ◽  
Yeong Shik Kim ◽  
Soon Sung Lim ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Prostaglandin E2 ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Murine Macrophage ◽  
Inhibitory Effects ◽  
Belamcanda Chinensis
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