Determination of nimesulide in pharmaceutical dosage forms by second order derivative UV spectrophotometry

A rapid and simple method for simultaneous determination of Chlorpheniramine Maleate (CPM) and Phenylpropanolamine Hydrochloride (PPM) by first derivative UV spectrophotometry has been developed in combined pharmaceutical dosage forms. The proposed method was successfully applied for the determination of drugs in physical mixture and commercial formulations and results showed good linearity, precision and reproducibility. DOI: http://dx.doi.org/10.3329/icpj.v2i5.14436 International Current Pharmaceutical Journal, April 2013, 2(5): 97-100

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Estimation of Tadalafil Using Derivative Spectrophotometry in Bulk Material and in Pharmaceutical Formulation

International Journal of Spectroscopy ◽

10.1155/2014/392421 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 5

Author(s):

Zamir G. Khan ◽

Amod S. Patil ◽

Atul A. Shirkhedkar

Keyword(s):

Wavelength Range ◽

Area Under Curve ◽

Pharmaceutical Formulation ◽

Second Order ◽

Order Derivative ◽

Uv Spectrophotometry ◽

First Order ◽

Beer's Law ◽

Beer’S Law ◽

Derivative Uv Spectrophotometry

Four simple, rapid, accurate, precise, reliable, and economical UV-spectrophotometric methods have been proposed for the determination of tadalafil in bulk and in pharmaceutical formulation. “Method A” is first order derivative UV spectrophotometry using amplitude, “method B” is first order derivative UV spectrophotometry using area under curve technique, “method C” is second order derivative UV spectrophotometry using amplitude, and “method D” is second order derivative UV spectrophotometry using area under curve technique. The developed methods have shown best results in terms of linearity, accuracy, precision, and LOD and LOQ for bulk drug and marketed formulation as well. In N,N-dimethylformamide, tadalafil showed maximum absorbance at 284 nm. For “method A” amplitude was recorded at 297 nm while for “method B” area under curve was integrated in the wavelength range of 290.60–304.40 nm. For “method C” amplitude was measured at 284 nm while for “method D” area under curve was selected in the wavelength range of 280.80–286.20 nm. For methods A and B, tadalafil obeyed Lambert-Beer’s law in the range of 05–50 μg/mL while for “methods C and D”, tadalafil obeyed Lambert-Beer’s law in the range of 20–70 μg/mL, and-for “methods A, B, C, and D” the correlation coefficients were found to be > than 0.999.

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SIMULTANEOUS ESTIMATION AND VALIDATION OF RAMIPRIL AND TELMISARTAN BY UV SPECTROPHOTOMETRY

INDIAN DRUGS ◽

10.53879/id.51.09.10156 ◽

2014 ◽

Vol 51 (09) ◽

pp. 31-35

Author(s):

R Rambabu ◽

◽

S Vidyadhara ◽

J Subbarao

Keyword(s):

Spectrophotometric Method ◽

Correlation Coefficients ◽

Simultaneous Equation ◽

Dosage Forms ◽

Simultaneous Estimation ◽

Uv Spectrophotometry ◽

Intermediate Precision ◽

Pharmaceutical Dosage Forms ◽

Sensitive Spectrophotometric Method

A simple and sensitive spectrophotometric method for the determination of ramipril and telmisartan in pharmaceutical dosage forms has been developed. The absorption maxima were found at 220nm for ramipril and 297nm for telmisartan using 0.1N NaOH as solvent. Beer’s law was obeyed for both the drugs in the concentration range of 2-10μg/ml with correlation coefficients 0.999 for both ramipril and telmisartan. The limits of detection for ramipril and telmisartan were found to be 0.142 and 0.405μg/mL respectively and the limits of quantitation were 0.43 and 1.22μg/mL. Accuracy of the method was verified by performing recovery studies using simultaneous equation method and found to be 98.33 to 99.54%w/w for ramipril and 99.36 to 99.82 %w/w for telmisartan. %RSD of repeatability and intermediate precision studies were found to be <2 for both the drugs. Ruggedness of the method was checked by changing analyst worked and instrument used. In both the cases, the %RSD was found to be less than 2.

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Estimation of Tramadol Hydrochloride in Bulk and Formulation by Second Order Derivative Area Under Curve UV-Spectrophotometric Methods.

International Journal of Advances in Scientific Research ◽

10.7439/ijasr.v1i7.1989 ◽

2015 ◽

Vol 1 (7) ◽

pp. 308

Author(s):

Rekha Sudam Kharat

Keyword(s):

Area Under Curve ◽

Pharmaceutical Formulations ◽

Second Order ◽

Order Derivative ◽

Tramadol Hydrochloride ◽

Pharmaceutical Dosage Forms ◽

Mean Values ◽

Spectrophotometric Methods ◽

Significant Difference

Simple, fast and reliable spectrophotometric methods were developed for determination of Tramadol Hydrochloride in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Distilled Water. The quantitative determination of the drug was carried out using the second order Derivative Area under Curve method values measured at 272-280nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Tramadol Hydrochloride using 2-10?g/ml (r=0.9925) for second order Derivative Area under Curve spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The developed methods were successfully applied to estimate the amount of Tramadol Hydrochloride in pharmaceutical formulations.

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Estimation of Ofloxacin in Bulk and Formulation by Second Order DerivativeUV-Spectrophotometric Methods

International Journal of Advances in Scientific Research ◽

10.7439/ijasr.v1i5.1977 ◽

2015 ◽

Vol 1 (5) ◽

pp. 217

Author(s):

Shivaji Shinde ◽

Santosh Jadhav ◽

Rekha Kharat ◽

Afaque Ansari ◽

Ashpak Tamboli

Keyword(s):

Area Under Curve ◽

Pharmaceutical Formulations ◽

Second Order ◽

Order Derivative ◽

Pharmaceutical Dosage Forms ◽

Mean Values ◽

Spectrophotometric Methods ◽

Ich Guidelines ◽

Significant Difference

Simple, fast and reliable spectrophotometric methods were developed for determination of Ofloxacin in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Methanol. The quantitative determination of the drug was carried out using the second order Derivative Area under Curve method values measured at 295-301nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Ofloxacin using 2-10?g/ml (r=0.9947) for second order Derivative Area under Curve spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The developed methods were successfully applied to estimate the amount of Ofloxacin in pharmaceutical formulations.

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