The duration and quality of positive direct immunofluorescence in skin biopsies using michel's fixative on a case of equine pemphigus foliaceus

1983 ◽  
Vol 3 (1) ◽  
pp. 14-17 ◽  
Author(s):  
Edmund J. Rosser ◽  
Peter J. Ihrke ◽  
Stephen D. White ◽  
Anthony A. Stannard ◽  
Alex Ardans
Keyword(s):  
Direct Immunofluorescence ◽  
Pemphigus Foliaceus ◽  
Skin Biopsies ◽  
Positive Direct

scholarly journals Clinical, histopathological and immunofluorescent study of vesicobullous lesions of skin

2019 ◽  
Vol 7 (4) ◽  
pp. 1288
Author(s):  
Seeram Satish Kumar ◽  
Bhagyalakshmi Atla ◽  
Guru Prasad Patnala ◽  
Kukkala Saraswati Sarat Srinivas ◽  
Saraswathi Samantra ◽  
...  
Keyword(s):  
Bullous Pemphigoid ◽  
Histopathological Examination ◽  
Pemphigus Vulgaris ◽  
Direct Immunofluorescence ◽  
Pemphigus Foliaceus ◽  
Female Ratio ◽  
Medical College ◽  
Intensive Investigation ◽  
Male To Female

Background: Vesiculobullous diseases have been the focus of intensive investigation in recent years. However, these disorders are still associated with substantial morbidity, considerable mortality and impaired quality of life. Accurate diagnosis of vesiculobullous lesions of skin entails evaluation of clinical, histopathologic and immunofluorescence findings.Methods: Hospital based prospective study for a period of 24 months from August 2014 to July 2016 in the Department of Pathology at Andhra Medical College, Visakhapatnam, India. Total of 50 patients aged 3-70 years with vesiculobullous lesions of both sexes attending the Department of Dermatology were selected and analysed clinically, histopathological examination and direct immunofluorescence (DIF).Results: In the present study, majority of patients presented between 51-60 yrs of age (32%) with male to female ratio of 1.08:1 and mean age of 46.02 years. Pemphigus vulgaris constituted the most common vesiculobullous disorder (32%) followed by bullous pemphigoid and pemphigus foliaceous, 18% each. Bullae were located intra epidermally in 68% and sub epidermally in 32% of the patients. DIF was positive in 80% of the cases. Overall clinicopathological correlation was established in 74%. Overall histopathological and direct immunofluorescence correlation was established in 78%. Out of 50 cases, 35 cases (70%) correlated clinically and histo-pathologically with direct immunofluorescence.Conclusions: In the present study, on histopathological examination alone pemphigus foliaceus and pemphigus vulgaris could be differentiated. Direct immunofluorescence was useful in differentiating epidermolysis bullosa acquisita from bullous pemphigoid which have similar histopathological picture. This study proves that direct immunofluorescence is confirmatory as well as diagnostic for vesiculobullous disorders.

scholarly journals An open-label study to evaluate biomarkers and safety in systemic sclerosis patients treated with paquinimod

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Roger Hesselstrand ◽  
Jörg H. W. Distler ◽  
Gabriela Riemekasten ◽  
Dirk M. Wuttge ◽  
Marie Törngren ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Type I ◽  
Type I Ifn ◽  
Open Label ◽  
Reactive Protein ◽  
Before And After ◽  
Modified Rodnan Skin Score ◽  
Skin Biopsies ◽  
Rank 2

Abstract Objectives To evaluate the changes in disease-related biomarkers and safety of paquinimod, an oral immunomodulatory compound, in patients with systemic sclerosis (SSc). Methods In this open-label, single-arm, multicenter study, SSc patients with a rapidly progressive disease received paquinimod for 8 weeks. Blood and skin biopsies were collected at baseline, during treatment, and at follow-up for the analyses of type I interferon (IFN) activity, chemokine (C-C motif) ligand 2 (CCL2), and the number of myofibroblasts. The safety of paquinimod was evaluated throughout the study. Results Nine SSc patients were enrolled and completed the study treatment with paquinimod at 3 mg/day for 8 weeks. After the treatment, a reduction of type I IFN activity in the plasma from one patient with elevated baseline IFN activity was recorded. A trend towards reduced IFN activity in the skin after treatment was also observed in patients. The serum level of CCL2 was reduced in 7 of 9 patients after paquinimod treatment. There was a median reduction of 10% of the number of myofibroblasts in skin biopsies at week 8 compared to baseline. No change in modified Rodnan skin score and quality of life was detected in the study. Reported adverse events (AEs) were mild to moderate and expected with the most common being arthralgia (n = 3) and headache (n = 3), and C-reactive protein (CRP) increase. Conclusions Analysis of biomarkers before and after treatment suggest reduced type I IFN activity and reduced number of myofibroblasts in lesional skin. Paquinimod was overall well tolerated with mild to moderate and expected AEs. Trial registration ClinicalTrials.gov, NCT01487551. Registered on 7 September 2011

scholarly journals IMPACT OF EDUCATION AND HEALTH ON THE UNEMPLOYMENT RATE AND ECONOMY OF EAST JAVA

2019 ◽  
Vol 9 (2) ◽  
Author(s):  
Suyanto Suyanto ◽  
Bambang Purnomo ◽  
Rahmawati Erma StandsyahStandsyah
Keyword(s):  
Direct Effect ◽  
Unemployment Rate ◽  
Indirect Effect ◽  
Social Problems ◽  
Total Population ◽  
Insignificant Effect ◽  
Education And Health ◽  
Positive Direct

East Java statistics indicate that the total population of East Java Province is increasing every year. The increase in the population is followed by an increase in social problems, including unemployment. The unemployment rate can affect the economy of the society. Efforts must be made to reduce it by improving the quality of society through factors related to education and health. According to data from East Java in 2017, East Java's education has a significant positive direct effect on the East Java economy of 0.343%, while indirectly through unemployment has an insignificant effect increased by 0.0021%. In addition, health has no significant direct and indirect effect on the East Java economy with coefficients of 0.078% and -0.0023%, respectively.

scholarly journals The Significance of Scalp Involvement in Pemphigus: A Literature Review

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Marta Sar-Pomian ◽  
Lidia Rudnicka ◽  
Malgorzata Olszewska
Keyword(s):  
Web Of Science ◽  
Clinical Manifestations ◽  
Pemphigus Vulgaris ◽  
Hair Follicles ◽  
Response To Treatment ◽  
Direct Immunofluorescence ◽  
Pemphigus Foliaceus ◽  
Medical Databases ◽  
Scalp Lesions ◽  
Reliable Diagnostic Method

Scalp is a unique location for pemphigus because of the abundance of desmogleins localized in hair follicles. Scalp involvement is observed in up to 60% of patients in the course of pemphigus. The lesions may occasionally lead to alopecia. Unforced removal of anagen hairs in a pull test is a sign of high disease activity. Direct immunofluorescence of plucked hair bulbs is considered a reliable diagnostic method in patients with pemphigus. Follicular acantholysis is a characteristic histopathological feature of pemphigus lesions localized on the scalp. Trichoscopy may serve as a supplementary method in the diagnosis of pemphigus. This review summarizes the most recent data concerning scalp involvement in pemphigus vulgaris and pemphigus foliaceus. A systematic literature search was conducted in three medical databases: PubMed, Embase, and Web of Science. The analysis included literature data about desmoglein distribution in hair follicles, as well as information about clinical manifestations, histopathology, immunopathology, and trichoscopy of scalp lesions in pemphigus and their response to treatment.

scholarly journals 176 Measuring quality of life with the EQ-5D in patients with pemphigus vulgaris and pemphigus foliaceus

2019 ◽  
Vol 139 (9) ◽  
pp. S244
Author(s):  
B. Tamasi ◽  
V. Brodszky ◽  
M. Pentek ◽  
L. Gulacsi ◽  
K. Hajdu ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Pemphigus Vulgaris ◽  
Pemphigus Foliaceus

Positive direct immunofluorescence and autoantibody profiles in psoriasis patients

2008 ◽  
Vol 35 (8) ◽  
pp. 508-513 ◽  
Author(s):  
Patcharin JANJUMRATSANG ◽  
Daraka PHAINUPONG ◽  
Somchai CHANJANAKIJSKUL ◽  
Paisal ROONGPHIBULSOPIT
Keyword(s):  
Direct Immunofluorescence ◽  
Positive Direct ◽  
Autoantibody Profiles

scholarly journals Exfoliative dermatitis causing diagnostic dilemma: Sibling provides the missing link

2020 ◽  
Vol 2 ◽  
pp. 40-42
Author(s):  
Soumya Jagadeesan ◽  
Malini Eapen ◽  
Aditi Karunakaran ◽  
Vinitha Panicker ◽  
Gopikrishnan Anjaneyan ◽  
...  
Keyword(s):  
Skin Biopsy ◽  
Index Case ◽  
Direct Immunofluorescence ◽  
Pemphigus Foliaceus ◽  
Pathological Features ◽  
Immunofluorescence Test ◽  
Diagnostic Dilemma ◽  
Histopathological Features ◽  
Exfoliative Dermatitis ◽  
Mucosal Lesions

A 49-year-old male presented with erythroderma of 3-month duration with a flexural onset. No mucosal lesions were present, and skin biopsy showed features of acantholysis and suprabasal clefting. Direct immunofluorescence test was negative in repeated examinations. The patient was presumptively managed as pemphigus foliaceus with immunomodulators though the level of split was suprabasal rather than subcorneal, and the patient responded well to the treatment. However, his elder sister presented to the department with clinical and histopathological features of classical Hailey–Hailey disease (HHD) which resembled the pathological features found in the index case. This prompted us to review the diagnosis and retrospectively diagnose the index case as HHD presenting with erythroderma.

scholarly journals HISTOPATHOLOGICAL AND IMMUNOFLUORESCENT STUDIES IN CERTAIN SKIN DISORDERS

2019 ◽  
Vol 3 (8) ◽  
Author(s):  
Rekha S ◽  
Vineet Kumar ◽  
Vanita Kumar ◽  
R D Mehta
Keyword(s):  
Lichen Planus ◽  
Dermatitis Herpetiformis ◽  
Direct Immunofluorescence ◽  
Skin Disorders ◽  
Immunological Mechanism ◽  
Medical College ◽  
Skin Biopsy Specimen ◽  
Immunofluorescent Technique ◽  
Skin Biopsies

Background: This study was an attempt to evaluate the role of direct immunofluorescent technique to demonstrate the immunoglobulins in certain skin disorders, which are likely to have immunological mechanism in their pathogenesis. Methods: This study was carried out in department of Pathology, Sardar Patel Medical College & Associated group of Hospitals, Bikaner. This study was hospital based study on skin biopsy specimen recieved in the department of Pathology during the study period. Results: On immunofluorescent studies of these skin biopsies, 12 out of 16 cases of pemphigus were positive for fluorescence was intracellular area in epidermis. In lichen planus  5 out of 7 cases were positive for fluorescence and commonest site was dermo-epideremal junction. In dermatis-herpatiformis 2 out of 3cases were positive for fluorescence and commonest site was dermo-epideremal junction. IgG was commonest type of immunoglobulin’s demonstrate in 12 out of 16 cases of pemphigus followed by IgM (5 cases), IgA(1cases). In lichen planus  IgM was commonest type of immunoglobulin’s demonstrate in 5 out of 7 cases of pemphigus followed by IgG (3 cases), IgA(1cases). In dermatitis herpatiformis IgA was commonest type of immunoglobulin’s demonstrate in 2 out of 3 cases of pemphigus followed by IgM (1 cases). Conclusion: We conclude that the demonstration of immunoglobulins in skin biopsies by direct fluorescent technique is a quite useful adjunct in diagnostic confirmation of pemphigus, lichen planus and dermatitis herpetiformis. Keywords: Direct immunofluorescence, Pemphigus, Histopathology.

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