GROWTH FACTOR ACTION ON OVARIAN FUNCTION IN POLYCYSTIC OVARY SYNDROME

In order to explore the possible treatment mechanism of metformin on the local ovarian cell tissue of rats with polycystic ovary syndrome (PCOS), 35 female clean sterile rats were selected as the research objects in this study, and randomly divided a PCOS model group (PCOS MG) (25 rats) and a control group (CG) (10 rats). After the modelling was completed, 5 rats were randomly selected to evaluate the modelling effect. When the success rate was higher than 80%, the remaining model rats were divided into two groups randomly, namely the (PCOS MG) (10 rats) and the treatment group (TG) (10 rats). Hematoxylin-eosin (HE) staining was performed on ovarian tissue of the rat, and the ovarian tissue structure was observed under light microscope. Immunohistochemistry was used to detect the distribution and expression levels of tumour necrosis factor-α (TNF-α), insulin-like growth factor-I (IGF-I), and connective tissue growth factor (CTGF) on the ovaries of rats in each group. It was found by observing the vaginal smear under the microscope that the rats in the (PCOS MG) had lost the regular estrous cycle, suggesting that there was no ovulation. The expression levels of TNF-α and CTGF in rats in the (PCOS MG) were greatly higher than those in the CG (P < 0.05); compared with the (PCOS MG), the expression levels of TNF-α and CTGF in the TG were decreased observably (P < 0.05). IGF-I was mainly expressed in granulosa cells (GCs) and follicular membrane cells (FMCs) of the ovarian tissue. The expression level of IGF-I in ovarian GCs in rats in the (PCOS MG) was significantly higher than that in the CG (P < 0.05). The expression level of IGF-I in GCs in the TG was lower significantly than that in the (PCOS MG) (P < 0.05). By comparing with rats in the CG, the rats in the (PCOS MG) had obviously decreased Actinobacteria and Betaproteobacteria in the intestinal tract, and the proportion of Firmicutes in the intestine was significantly increased; the amount of butyric acid in the faeces of rats with aerobic exercise was obviously higher than that in the (PCOS MG), because exercise increased the proportion of intestinal butyric acid-producing bacteria. Conclusion: metformin combined with aerobic exercise can treat the PCOS by regulating serum hormone levels and the expression levels of TNF-α, IGF-I, and CTGF.

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Use of Inositols in Polycystic Ovary Syndrome

Effective Pharmacotherapy ◽

10.33978/2307-3586-2020-16-28-24-34 ◽

2020 ◽

Vol 16 (28) ◽

pp. 24-34

Author(s):

O.A. Pustotina ◽

Keyword(s):

Polycystic Ovary Syndrome ◽

Ovarian Function ◽

Polycystic Ovary ◽

The Body ◽

Metabolic Parameters ◽

Ovary Syndrome ◽

Pathogenetic Role

The article presents key data on the physiology of inositols in the body, their pathogenetic role in the development of polycystic ovary syndrome, and the possibilities of myo-inositol and D-chiro-inositol in the restoration of ovarian function, metabolic parameters, and overcoming of infertility.

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Rosiglitazone in insulin-resistant women with polycystic ovary syndrome (PCOS): effects on ovarian function and metabolism

Fertility and Sterility ◽

10.1016/s0015-0282(02)03474-x ◽

2002 ◽

Vol 78 ◽

pp. S36 ◽

Cited By ~ 1

Author(s):

Nicholas A Cataldo ◽

Fahim Abbasi ◽

Tracey L McLaughlin ◽

Patricia Y Fechner ◽

Gerald M Reaven ◽

...

Keyword(s):

Polycystic Ovary Syndrome ◽

Ovarian Function ◽

Polycystic Ovary ◽

Ovary Syndrome ◽

Insulin Resistant

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Hyperexpression of epidermal growth factor receptors in granulosa cells from women with polycystic ovary syndrome

Fertility and Sterility ◽

10.1016/s0015-0282(98)00252-0 ◽

1998 ◽

Vol 70 (4) ◽

pp. 750-758 ◽

Cited By ~ 10

Author(s):

Ghanim Almahbobi ◽

Aileen Misajon ◽

Paul Hutchinson ◽

Nicholas Lolatgis ◽

Alan O Trounson

Keyword(s):

Growth Factor ◽

Epidermal Growth Factor ◽

Polycystic Ovary Syndrome ◽

Granulosa Cells ◽

Polycystic Ovary ◽

Growth Factor Receptors ◽

Epidermal Growth Factor Receptors ◽

Ovary Syndrome ◽

Epidermal Growth

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Dysregulated miR-142, -33b and -423 in granulosa cells target TGFBR1 and SMAD7: a possible role in polycystic ovary syndrome

MHR Basic science of reproductive medicine ◽

10.1093/molehr/gaz014 ◽

2019 ◽

Vol 25 (10) ◽

pp. 638-646 ◽

Cited By ~ 11

Author(s):

Yan Li ◽

Yungai Xiang ◽

Yuxia Song ◽

Lijing Wan ◽

Guo Yu ◽

...

Keyword(s):

Cell Proliferation ◽

Growth Factor ◽

Polycystic Ovary Syndrome ◽

Granulosa Cells ◽

Transforming Growth Factor Beta ◽

Transforming Growth Factor ◽

Polycystic Ovary ◽

Ovary Syndrome ◽

Growth Factor Beta ◽

Tgfb Signaling

Abstract It is well established that microRNA (miRNA) expression profiles are altered in patients with polycystic ovary syndrome (PCOS). In addition, abnormal transforming growth factor beta (TGFB) signaling in granulosa cells is related to the pathological conditions of PCOS. However, the function of dysregulated miRNAs in PCOS is still unclear. In this study, we aimed to elucidate the roles of specific miRNAs in PCOS. We collected follicular fluid from 46 patients with PCOS and 32 healthy controls. Granulosa cells (GCs) were separated and the levels of six candidate miRNAs were determined by quantitative RT-PCR. The direct targets of three dysregulated miRNAs were predicted using bioinformatic tools and confirmed using a dual luciferase assay and immunoblotting. The biological function of three dysregulated miRNAs in primary GCs was determined using a cell proliferation assay and flow cytometry. We found that miR-423 expression was downregulated (P = 0.038), and the levels of miR-33b (P = 0.032) and miR-142 (P = 0.021) were upregulated in GCs from patients with PCOS, compared to controls. miR-423 directly repressed SMAD family member 7 (SMAD7) expression, while transforming growth factor beta receptor 1 (TGFBR1) was a direct target of both miR-33b and miR-142. An RNA oligonucleotide mixture containing miR-423 inhibitor, miR-33b mimic, and miR-142 mimic repressed TGFB signaling, promoted cell proliferation (P = 0.0098), repressed apoptosis (P = 0.027), and increased S phase cell numbers (P = 0.0036) in primary cultures of GCs, compared to the cells treated with a sequence scrambled control RNA oligonucleotide. This study unveiled the possible roles of three miRNAs in PCOS and might provide candidate biomarkers for PCOS diagnosis while in vivo functional studies, using transgenic or knockout mouse models, are expected to confirm the roles of dysregulated miRNAs in the pathogenesis of PCOS.

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