Induction of Bone Matrix Protein Expression by Native Bone Matrix Proteins in C2C12 Culture

In the present study, we evaluated the possibility that the abnormal bone matrix produced during spaceflight may be associated with reduced expression of bone matrix protein genes. To test this possibility, we investigated the effects of a 14-day spaceflight (SLS-2 experiment) on steady-state mRNA levels for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), osteocalcin, osteonectin, and prepro-α(1) subunit of type I collagen in the major bone compartments of rat femur. There were pronounced site-specific differences in the steady-state levels of expression of the mRNAs for the three bone matrix proteins and GAPDH in normal weight-bearing rats, and these relationships were altered after spaceflight. Specifically, spaceflight resulted in decreases in mRNA levels for GAPDH (decreased in proximal metaphysis), osteocalcin (decreased in proximal metaphysis), osteonectin (decreased in proximal and distal metaphysis), and collagen (decreased in proximal and distal metaphysis) compared with ground controls. There were no changes in mRNA levels for matrix proteins or GAPDH in the shaft and distal epiphysis. These results demonstrate that spaceflight leads to site- and gene-specific decreases in mRNA levels for bone matrix proteins. These findings are consistent with the hypothesis that spaceflight-induced decreases in bone formation are caused by concomitant decreases in expression of genes for bone matrix proteins.

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Biomembranes enriched with TGF?1 favor bone matrix protein expression by human osteoblastsin vitro

Journal of Biomedical Materials Research ◽

10.1002/jbm.10301 ◽

2002 ◽

Vol 63 (5) ◽

pp. 577-582 ◽

Cited By ~ 11

Author(s):

C. Lilli ◽

L. Marinucci ◽

G. Stabellini ◽

S. Belcastro ◽

E. Becchetti ◽

...

Keyword(s):

Protein Expression ◽

Matrix Protein ◽

Bone Matrix ◽

Bone Matrix Protein

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Osteomalacia in Hyp Mice Is Associated with Abnormal Phex Expression and with Altered Bone Matrix Protein Expression and Deposition1

Endocrinology ◽

10.1210/endo.142.2.7976 ◽

2001 ◽

Vol 142 (2) ◽

pp. 926-939 ◽

Cited By ~ 78

Author(s):

Dengshun Miao ◽

Xiuying Bai ◽

Dibyendu Panda ◽

Marc D. McKee ◽

Andrew C. Karaplis ◽

...

Keyword(s):

Protein Expression ◽

Matrix Protein ◽

Bone Matrix ◽

Bone Matrix Protein

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Promotion of a Breast Cancer Growth Within Bone by the C-Terminal Hexapeptide Enzymatically Derived From Osteocalcin, a Bone Matrix Protein

10.21236/ada419637 ◽

2003 ◽

Author(s):

Satoru K. Nishimoto

Keyword(s):

Breast Cancer ◽

Matrix Protein ◽

Bone Matrix ◽

Cancer Growth ◽

Breast Cancer Growth ◽

Bone Matrix Protein

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Interactions between the bone matrix proteins osteopontin and bone sialoprotein and the osteoclast integrin alpha v beta 3 potentiate bone resorption

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)98430-9 ◽

1993 ◽

Vol 268 (13) ◽

pp. 9901-9907

Author(s):

F.P. Ross ◽

J. Chappel ◽

J.I. Alvarez ◽

D. Sander ◽

W.T. Butler ◽

...

Keyword(s):

Bone Resorption ◽

Bone Matrix ◽

Bone Sialoprotein ◽

Matrix Proteins ◽

Bone Matrix Proteins ◽

Alpha V Beta 3

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Effect of protein restriction on the messenger RNA contents of bone-matrix proteins, insulin-like growth factors and insulin-like growth factor binding proteins in femur of ovariectomized rats

British Journal Of Nutrition ◽

10.1079/bjn19960188 ◽

1996 ◽

Vol 75 (6) ◽

pp. 811-823 ◽

Cited By ~ 10

Author(s):

Yusuke Higashi ◽

Asako Takenaka ◽

Shin-Ichiro Takahashi ◽

Tadashi Noguchi

Keyword(s):

Dietary Protein ◽

Type I Collagen ◽

Control Diet ◽

Bone Matrix ◽

Protein Restriction ◽

Matrix Proteins ◽

Type I ◽

Bone Matrix Proteins ◽

Mrna Content ◽

Insulin Like Growth Factors

It has been reported that loss of ovarian oestrogen after menopause or by ovariectomy causes osteoporosis. In order to elucidate the effect of dietary protein restriction on bone metabolism after ovariectomy, we fed ovariectomized young female rats on a casein-based diet (50g/kg diet (protein restriction) or 200g/kg diet (control)) for 3 weeks and measured mRNA contents of bone-matrix proteins such as osteocalcin, osteopontin and α1 type I collagen, insulin-like growth factors (IGF) and IGF-binding proteins (IGFBP) in femur. Ovariectomy decreased the weight of fat-free dry bone and increased urinary excretion of pyridinium cross-links significantly, although dietary protein restriction did not affect them. Neither ovariectomy nor protein restriction affected the content of mRNA of osteopontin and osteocalcin; however, ovariectomy increased and protein restriction extensively decreased the α1 type I collagen mRNA content in bone tissues. Ovariectomy increased IGF-I mRNA only in the rats fed on the control diet. Conversely, protein rest riction increased and ovariectomy decreased the IGF-II mRNA content in femur. Furthermore, the contents of IGFBP-2, IGFBP-4 and IGFBP-5 mRNA increased, but the content of IGFBP-3 mRNA decreased in femur of the rats fed on the protein-restricted diet. In particular, ovariectomy decreased the IGFBP-2 mRNA content in the protein-restricted rats and the IGFBP-6 mRNA content in the rats fed on the control diet. These results clearly show that the mRNA for some of the proteins which have been shown to be involved in bone formation are regulated by both quantity of dietary proteins and ovarian hormones.

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