Autologous platelet-rich plasma (PRP) is more useful than fetal calf serum (FCS) in adipose-derived stem cells (ADSCS) culture for bone regeneration

2005 ◽  
Vol 34 ◽  
pp. 123 ◽  
Author(s):  
S. Watatani ◽  
H. Terashi ◽  
K. Saigo ◽  
M. Yokoyama ◽  
M. Yamamoto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Fetal Calf Serum ◽  
Platelet Rich Plasma ◽  
Calf Serum ◽  
Adipose Derived Stem Cells ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet

scholarly journals The Effect of Autologous Platelet-Rich Plasma on Bone Regeneration by Autologous Mesenchymal Stem Cells Loaded onto Allogeneic Cancellous Bone Granules

2017 ◽  
Vol 203 (6) ◽  
pp. 327-338 ◽  
Author(s):  
Min Wook Joo ◽  
Seung Jae Chung ◽  
Seung Han Shin ◽  
Yang-Guk Chung
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Cancellous Bone ◽  
Platelet Rich Plasma ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Control Group ◽  
Micro Ct ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet

To develop a clinically effective bone regeneration strategy, we compared bone regeneration using allogeneic cancellous bone granule scaffolds loaded with autologous bone marrow-derived mesenchymal stem cells (BM-MSC) with or without autologous platelet-rich plasma (PRP). Critical-sized segmental bone defects were made at the mid-shaft of both radiuses in 41 New Zealand White rabbits. Small-sized allogeneic cancellous bone granules (300-700 μm in diameter) loaded with BM-MSC were implanted on one side, and PRP was added. On the other side, autologous BM-MSC loaded onto allogeneic cancellous granules were grafted as a control. Bone regeneration was assessed by radiographic evaluation at 4, 8, and 16 weeks postimplantation and by micro-computed tomography (micro-CT) and histological evaluation of the retrieved specimens at 8 and 16 weeks. The experimental group did not show significantly higher bone quantity indices than the control group at any time point. Micro-CT analysis revealed that both groups had similar mean total volumes, surface areas, and other parameters at 8 and 16 weeks. Histological evaluation of 8- and 16-week specimens also showed a similar progression of new bone formation and maturation. In this experiment using a contralateral control group in the same individual, an initial single addition of PRP in allogeneic cancellous bone granules loaded with BM-MSC for critical-sized bone defects in the weight-bearing area did not induce a consequent difference in bone healing. Further research into the optimal preparation and application of PRP is necessary. Furthermore, studies involving a greater number of subjects and larger experimental animals could determine the clinical relevance of PRP treatment.

Direct and Indirect Effects of a Combination of Adipose-Derived Stem Cells and Platelet-Rich Plasma on Bone Regeneration

2015 ◽  
Vol 21 (5-6) ◽  
pp. 895-905 ◽  
Author(s):  
Satoshi Tajima ◽  
Morikuni Tobita ◽  
Hakan Orbay ◽  
Hiko Hyakusoku ◽  
Hiroshi Mizuno
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Indirect Effects ◽  
Platelet Rich Plasma ◽  
Adipose Derived Stem Cells ◽  
Direct And Indirect Effects

scholarly journals Bone Regeneration Using Adipose-Derived Stem Cells in Injectable Thermo-Gelling Hydrogel Scaffold Containing Platelet-Rich Plasma and Biphasic Calcium Phosphate

2018 ◽  
Vol 19 (9) ◽  
pp. 2537 ◽  
Author(s):  
Han Liao ◽  
Ming-Jin Tsai ◽  
Manuri Brahmayya ◽  
Jyh-Ping Chen
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Calcium Phosphate ◽  
Bone Regeneration ◽  
Biphasic Calcium Phosphate ◽  
Platelet Rich Plasma ◽  
Bone Matrix ◽  
Calcium Deposition ◽  
Adipose Derived Stem Cells ◽  
Hydrogel Scaffold

For bone regeneration, a biocompatible thermo-gelling hydrogel, hyaluronic acid-g-chitosan-g-poly(N-isopropylacrylamide) (HA-CPN) was used as a three-dimensional organic gel matrix for entrapping rabbit adipose-derived stem cells (rASCs). Biphasic calcium phosphate (BCP) ceramic microparticles were embedded within the gel matrix as a mineralized bone matrix, which was further fortified with platelet-rich plasma (PRP) with osteo-inductive properties. In vitro culture of rASCs in HA-CPN and HA-CPN/PRP/BCP was compared for cell proliferation and osteogenic differentiation. Overall, HA-CPN/PRP/BCP was a better injectable cell carrier for osteogenesis of rASCs with increased cell proliferation rate and alkaline phosphatase activity, enhanced calcium deposition and mineralization of extracellular matrix, and up-regulated expression of genetic markers of osteogenesis. By implanting HA-CPN/PRP/BCP/rASCs constructs in rabbit critical size calvarial bone defects, new bone formation at the defect site was successfully demonstrated from computed tomography, and histological and immunohistochemical analysis. Taken together, by combining PRP and BCP as the osteo-inductive and osteo-conductive factor with HA-CPN, we successfully demonstrated the thermo-gelling composite hydrogel scaffold could promote the osteogenesis of rASCs for bone tissue engineering applications.

Adipose-derived stem cells incorporated into platelet-rich plasma improved bone regeneration and maturationin vivo

2014 ◽  
Vol 31 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Ariadne Cristiane Cabral Cruz ◽  
Thiago Caon ◽  
Álvaro Menin ◽  
Rodrigo Granato ◽  
Fernanda Boabaid ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Platelet Rich Plasma ◽  
Adipose Derived Stem Cells
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