scholarly journals Autologous platelet-rich plasma promotes proliferation and chondrogenic differentiation of adipose-derived stem cells

2014 ◽  
Vol 11 (2) ◽  
pp. 1298-1303 ◽  
Author(s):  
JI SHEN ◽  
QINGFENG GAO ◽  
YAO ZHANG ◽  
YAOHUA HE
Keyword(s):  
Stem Cells ◽  
Chondrogenic Differentiation ◽  
Platelet Rich Plasma ◽  
Adipose Derived Stem Cells ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet

scholarly journals The Effect of Autologous Platelet-Rich Plasma on Bone Regeneration by Autologous Mesenchymal Stem Cells Loaded onto Allogeneic Cancellous Bone Granules

2017 ◽  
Vol 203 (6) ◽  
pp. 327-338 ◽  
Author(s):  
Min Wook Joo ◽  
Seung Jae Chung ◽  
Seung Han Shin ◽  
Yang-Guk Chung
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Cancellous Bone ◽  
Platelet Rich Plasma ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Control Group ◽  
Micro Ct ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet

To develop a clinically effective bone regeneration strategy, we compared bone regeneration using allogeneic cancellous bone granule scaffolds loaded with autologous bone marrow-derived mesenchymal stem cells (BM-MSC) with or without autologous platelet-rich plasma (PRP). Critical-sized segmental bone defects were made at the mid-shaft of both radiuses in 41 New Zealand White rabbits. Small-sized allogeneic cancellous bone granules (300-700 μm in diameter) loaded with BM-MSC were implanted on one side, and PRP was added. On the other side, autologous BM-MSC loaded onto allogeneic cancellous granules were grafted as a control. Bone regeneration was assessed by radiographic evaluation at 4, 8, and 16 weeks postimplantation and by micro-computed tomography (micro-CT) and histological evaluation of the retrieved specimens at 8 and 16 weeks. The experimental group did not show significantly higher bone quantity indices than the control group at any time point. Micro-CT analysis revealed that both groups had similar mean total volumes, surface areas, and other parameters at 8 and 16 weeks. Histological evaluation of 8- and 16-week specimens also showed a similar progression of new bone formation and maturation. In this experiment using a contralateral control group in the same individual, an initial single addition of PRP in allogeneic cancellous bone granules loaded with BM-MSC for critical-sized bone defects in the weight-bearing area did not induce a consequent difference in bone healing. Further research into the optimal preparation and application of PRP is necessary. Furthermore, studies involving a greater number of subjects and larger experimental animals could determine the clinical relevance of PRP treatment.

scholarly journals Comparative impact of platelet rich plasma and transforming growth factor-β on chondrogenic differentiation of human adipose derived stem cells

Bioimpacts ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Roya Hesari ◽  
Mina Keshvarinia ◽  
Mahboubeh Kabiri ◽  
Iman Rad ◽  
Kazem Parivar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Side Effects ◽  
Transforming Growth Factor Beta ◽  
Chondrogenic Differentiation ◽  
Transforming Growth Factor ◽  
Platelet Rich Plasma ◽  
Adipose Derived Stem Cells ◽  
Necrosis Factor Alpha

<span style="color: #1f497d;">Introduction: Transforming growth factor-beta (TGF-β) is known as standard chondrogenic differentiation agent, even though it comes with undesirable side effects such as early hypertrophic maturation, mineralization, and secretion of inflammatory/angiogenic factors. On the other hand, platelet-rich plasma (PRP) is found to have a chondrogenic impact on mesenchymal stem cell proliferation and differentiation, with no considerable side effects. Therefore, we compared chondrogenic impact of TGF-β and PRP on adipose-derived stem cells (ADSCs), to see if PRP could be introduced as an alternative to TGF-β. <br /> <span style="color: #1f497d;">Methods: Differentiation of ADSCs was monitored using a couple of methods including glycosaminoglycan production, miRNAs expression, vascular endothelial growth factor (VEGF)/tumor necrosis factor alpha (TNFα) secretion, alkaline phosphatase (ALP) and calcium content assays.<br /> <span style="color: #1f497d;">Results: Accordingly, the treatment of differentiating cells with 5% (v/v) PRP resulted in higher glycosaminoglycan production, enhanced SOX9 transcription, and lowered TNFα and VEGF secretion compared to the control and TGF-β groups. Besides, the application of PRP to the media up-regulated miR-146a and miR-199a in early and late stages of chondrogenesis, respectively. <span style="color: #1f497d;">Conclusion: PRP induces in vitro chondrogenesis, as well as TGF-β with lesser inflammatory and hypertrophic side effects.

scholarly journals Autologous Adipose-Derived Stem Cells, Platelet-Rich Plasma, and Fibrin Enhance Healing of Mandibular Bone Defects in Swine

2020 ◽  
pp. 1-9
Author(s):  
Matthew B. Wheeler ◽  
Aaron J. Maki ◽  
Anna C. Ercolin ◽  
Jamey J. Cooper ◽  
Kelly S. Roballo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Volume Fraction ◽  
Critical Size ◽  
Platelet Rich Plasma ◽  
Therapeutic Effects ◽  
Mineral Density ◽  
Bone Volume Fraction ◽  
Critical Size Defects ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet

The primary aim of this study was to assess the therapeutic effects of autologous platelet-rich plasma and fibrin scaffolds combined with autologous adipose stem cells (ASCs) for critical-size defects in the pig mandible. Fibrin scaffolds supplemented with calcium hydrogen phosphate and platelet-derived growth factors were hypothesized to accelerate healing in porcine mandible bone compared to ASC-only injections and untreated controls. Three treatments included the use of autologous ASCs from liposuction with the addition of platelet-rich plasma, fibrin scaffolds, or as cell-only controls. All three treatments using ASCs were determined to increase bone mineral density and bone volume fraction compared to untreated controls. In general, the addition of both platelet-rich plasma and fibrin scaffolds to autologous ASCs from liposuction improved bone healing of critical-size defects.

scholarly journals A novel treatment of radiation induced xerostomia by autologous platelet-rich-plasma and peripheral stem cells

2016 ◽  
Vol 27 ◽  
pp. vi348
Author(s):  
A. Taghizadeh Kermani ◽  
P. Izadpanahi ◽  
K. Khazaeni ◽  
M. Pezeshki Rad M ◽  
R. Asadi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Platelet Rich Plasma ◽  
Novel Treatment ◽  
Radiation Induced ◽  
Autologous Platelet Rich Plasma ◽  
Peripheral Stem Cells ◽  
Autologous Platelet
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