Effect of autologous platelet-rich plasma on the chondrogenic differentiation of rabbit adipose-derived stem cells in vitro

Introduction: Transforming growth factor-beta (TGF-β) is known as standard chondrogenic differentiation agent, even though it comes with undesirable side effects such as early hypertrophic maturation, mineralization, and secretion of inflammatory/angiogenic factors. On the other hand, platelet-rich plasma (PRP) is found to have a chondrogenic impact on mesenchymal stem cell proliferation and differentiation, with no considerable side effects. Therefore, we compared chondrogenic impact of TGF-β and PRP on adipose-derived stem cells (ADSCs), to see if PRP could be introduced as an alternative to TGF-β. Methods: Differentiation of ADSCs was monitored using a couple of methods including glycosaminoglycan production, miRNAs expression, vascular endothelial growth factor (VEGF)/tumor necrosis factor alpha (TNFα) secretion, alkaline phosphatase (ALP) and calcium content assays. Results: Accordingly, the treatment of differentiating cells with 5% (v/v) PRP resulted in higher glycosaminoglycan production, enhanced SOX9 transcription, and lowered TNFα and VEGF secretion compared to the control and TGF-β groups. Besides, the application of PRP to the media up-regulated miR-146a and miR-199a in early and late stages of chondrogenesis, respectively. Conclusion: PRP induces in vitro chondrogenesis, as well as TGF-β with lesser inflammatory and hypertrophic side effects.

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Autologous Platelet-Rich Plasma Treatment Enables Pregnancy for a Woman in Premature Menopause

Journal of Clinical Medicine ◽

10.3390/jcm8010001 ◽

2018 ◽

Vol 8 (1) ◽

pp. 1 ◽

Cited By ~ 10

Author(s):

Konstantinos Sfakianoudis ◽

Mara Simopoulou ◽

Nikolaos Nitsos ◽

Anna Rapani ◽

Athanasios Pappas ◽

...

Keyword(s):

In Vitro Fertilization ◽

Plasma Treatment ◽

Platelet Rich Plasma ◽

Ovarian Tissue ◽

Medical Knowledge ◽

Premature Menopause ◽

Vitro Fertilization ◽

Autologous Platelet Rich Plasma ◽

Autologous Platelet

This report presents the case of a woman aged 40 who has experienced premature menopause from the age of 35. Having rejected oocyte donation, she opted for intraovarian injection of autologous platelet-rich plasma with the aim to rejuvenate the ovarian tissue and enable the employment of her own gametes through in-vitro fertilization. Six weeks following the autologous platelet-rich plasma treatment, a significant reduction in the patient’s follicle-stimulating hormone (FSH) levels were noted. A natural in-vitro fertilization cycle led to a biochemical pregnancy, resulting in a spontaneous abortion at the 5th week of pregnancy. This is the first report of a successful autologous platelet-rich plasma application leading to pregnancy in menopause. This report uniquely contributes to the medical knowledge and challenges current practice in the context of infertility. The efficiency and safety of this treatment with regard to the reproductive system merits further investigation.

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Can Activated Platelet Rich Plasma Combined with Adipose-Derived Stem Cells Be Used to Treat Skin Wrinkles?

Medical Advancements in Aging and Regenerative Technologies - Advances in Medical Technologies and Clinical Practice ◽

10.4018/978-1-4666-2506-8.ch014 ◽

2013 ◽

pp. 313-329

Author(s):

Phuc Van Pham ◽

Loan Thi-Tung Dang ◽

Nhung Hai Truong ◽

Ngoc Kim Phan

Keyword(s):

Stem Cells ◽

Platelet Rich Plasma ◽

Adipose Derived Stem Cells ◽

Fibroblast Proliferation ◽

Skin Rejuvenation ◽

Collagen Production ◽

Dermal Layer ◽

Skin Wrinkles

In recent years, Platelet Rich Plasma (PRP) and Adipose-Derived Stem Cells (ADSCs) have been used separately for many clinical applications, especially skin rejuvenation. A combined injection of PRP and ADSCs could therefore be used to treat skin wrinkles. However, there are controversies and reports with conflicting results regarding the efficacy of this treatment. The authors aimed to determine the anti-wrinkle and skin rejuvenation mechanism of combined PRP and ADSCs treatment. The effects of PRP and ADSCs isolated from the same consenting donors were evaluated using in vitro and in vivo models. The in vitro effects of PRP and ADSCs on dermal fibroblast proliferation, collagen production, and inhibition of Matrix Metalloproteinase-1 (MMP-1) production were investigated using a co-culture model. Fibroblasts and ADSCs were cultured within the same dish, but in two separate cavities (using an insert plate), in the presence of the same PRP-supplemented medium. In vivo, the authors evaluated the effects of combined PRP and ADSCs on skin histochemistry, including changes in the dermal layer and collagen production in photo-aged skin (mice). They also determined the survival and differentiation of grafted ADSCs. The results show that combined PRP and ADSCs strongly stimulate in vitro fibroblast proliferation, collagen production, and inhibition of MMP-1 synthesis. Intra-dermal co-injection of PRP and ADSCs was observed to stimulate increased dermal layer thickness and collagen production compared with the untreated group. These results indicate that a combined PRP and ADSC injection can reduce wrinkles more effectively than either PRP or ADSC alone, and provide insight into the clinical use of PRP combined with ADSCs for dermal applications, particularly skin rejuvenation.

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In vitro effect of autologous platelet‐rich plasma on H 2 O 2 ‐induced oxidative stress in human spermatozoa

Andrology ◽

10.1111/andr.12648 ◽

2019 ◽

Vol 8 (1) ◽

pp. 191-200 ◽

Cited By ~ 1

Author(s):

R. Bader ◽

J. N. Ibrahim ◽

M. Moussa ◽

A. Mourad ◽

J. Azoury ◽

...

Keyword(s):

Oxidative Stress ◽

Platelet Rich Plasma ◽

Human Spermatozoa ◽

Autologous Platelet Rich Plasma ◽

Vitro Effect ◽

Autologous Platelet

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Platelet rich plasma hydrogels promote in vitro and in vivo angiogenic potential of adipose-derived stem cells

Acta Biomaterialia ◽

10.1016/j.actbio.2019.01.039 ◽

2019 ◽

Vol 87 ◽

pp. 76-87 ◽

Cited By ~ 16

Author(s):

Meghan Samberg ◽

Randolph Stone ◽

Shanmugasundaram Natesan ◽

Andrew Kowalczewski ◽

Sandra Becerra ◽

...

Keyword(s):

Stem Cells ◽

Platelet Rich Plasma ◽

Adipose Derived Stem Cells ◽

Angiogenic Potential

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Plasma Components and Platelet Activation Are Essential for the Antimicrobial Properties of Autologous Platelet-Rich Plasma: An In Vitro Study

PLoS ONE ◽

10.1371/journal.pone.0107813 ◽

2014 ◽

Vol 9 (9) ◽

pp. e107813 ◽

Cited By ~ 27

Author(s):

Lorenzo Drago ◽

Monica Bortolin ◽

Christian Vassena ◽

Carlo L. Romanò ◽

Silvio Taschieri ◽

...

Keyword(s):

Platelet Activation ◽

Platelet Rich Plasma ◽

Antimicrobial Properties ◽

In Vitro Study ◽

Vitro Study ◽

Autologous Platelet Rich Plasma ◽

Autologous Platelet

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Regenerative Effect of Intraovarian Injection of Activated Autologous Platelet Rich Plasma: Serum Anti-Mullerian Hormone Levels Measured Among Poor-Prognosis In Vitro Fertilization Patients

International Journal of Regenerative Medicine ◽

10.31487/j.rgm.2020.01.02 ◽

2020 ◽

pp. 1-5 ◽

Cited By ~ 2

Author(s):

E. Scott Sills ◽

J. L. Petersen ◽

N. S. Rickers ◽

Samuel H. Wood ◽

...

Keyword(s):

Clinical Trial ◽

In Vitro Fertilization ◽

Platelet Rich Plasma ◽

Ovarian Tissue ◽

Ovarian Response ◽

Vitro Fertilization ◽

Autologous Platelet Rich Plasma ◽

Pre Treatment ◽

Autologous Platelet

This registered, prospective clinical trial assessed serum anti-Mullerian hormone (AMH) patterns after treatment with activated platelet rich plasma (PRP). Patients with low ovarian reserve and/or at least 1 prior failed in vitro fertilization (IVF) cycle (n=182) received PRP injected into ovarian tissue under ultrasound guidance. Pretreatment AMH, BMI and platelet (PLT) concentration were recorded and serum AMH, follicle stimulating hormone, and estradiol were then measured at 2-week intervals for up to three months. Mean±SD patient age was 45.4±6.1yrs. Improved serum AMH was observed in 51 patients (28%) with median increase of 167% [95%CI 91; 280] after treatment; mean interval to maximum AMH increase was 4 weeks (range 2-10 weeks). Improved post-treatment AMH was not limited to younger patients; when stratified by age (<42 vs. ≥42yrs), significant AMH improvements were seen in both groups after treatment (p=0.03 and 0.009, respectively). Among responders, mean basal PLT count was higher (274K) vs. non-responders (250K); p<0.001. This is the first clinical trial to describe an intraovarian PRP technique for low reserve and finds the treatment safe and associated with significant increases in serum AMH for some patients, usually within four weeks. The substantially different pre-treatment PLT concentrations measured across PRP response groups warrants further investigation. Additional research can characterize ovarian response better, optimize PRP protocols, and collect outcomes data from those who subsequently undergo IVF with autologous oocytes.

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