P900 Performance of the GeneXpert ®system for detection of methicillin-resistant Staphylococcus aureus including the non-typeable clone from animal origin

2007 ◽  
Vol 29 ◽  
pp. S233-S234
Author(s):  
M.W.M. Wassenberg ◽  
S. Nijssen ◽  
X.W. Huijsdens ◽  
A.M.C. Bergmans ◽  
C.M.J.E. Vandenbroucke-Grauls ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Animal Origin ◽  
Methicillin Resistant

Molecular Characterization and Antimicrobial Resistance Profile of Methicillin-Resistant Staphylococcus aureus in Retail Chicken

2015 ◽  
Vol 78 (10) ◽  
pp. 1879-1884 ◽  
Author(s):  
KHALID IBRAHIM SALLAM ◽  
SAMIR MOHAMMED ABD-ELGHANY ◽  
MOHAMED ELHADIDY ◽  
TOMOHIRO TAMURA
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Meca Gene ◽  
Multidrug Resistant ◽  
Animal Origin ◽  
Contamination Rate ◽  
Methicillin Resistant ◽  
Food Of Animal Origin

The emergence of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) in food-producing animals is of increasing interest, raising questions about the presence of MRSA in food of animal origin and potential sources of transmission to humans via the food chain. In this study, the prevalence, molecular characterization, virulence factors, and antimicrobial susceptibility patterns of MRSA isolates from 200 retail raw chicken samples in Egypt were determined. MRSA was detected by positive amplification of the mecA gene in 38% (76 of 200) of chicken samples analyzed. This represents a potential public health threat in Egypt, as this contamination rate seems to be the highest among other studies reported worldwide. Furthermore, genes encoding α-hemolysin (hla) and staphylococcal enterotoxins (sea, seb, and sec) were detected in all of the 288 MRSA isolates. Nonetheless, none of the strains tested carried tst, the gene encoding toxic shock syndrome toxin 1. Antimicrobial resistance of MRSA isolates was most frequently detected against penicillin (93.4%), ampicillin (88.9%), and cloxacillin (83.3%). These results suggest that retail chicken might be a significant potential source for transmission of multidrug-resistant and toxigenic S. aureus in Egypt. This underlines the need for stricter hygienic measures in chicken production in Egypt to minimize the risk of transmission of these strains to consumers. To the best of our knowledge, this is the first study that reports the isolation and molecular characterization of MRSA in retail chicken samples in Egypt.

scholarly journals Detection of methicillin-resistant Staphylococcus aureus ST398 in food samples of animal origin in Spain

2009 ◽  
Vol 64 (6) ◽  
pp. 1325-1326 ◽  
Author(s):  
C. Lozano ◽  
M. Lopez ◽  
E. Gomez-Sanz ◽  
F. Ruiz-Larrea ◽  
C. Torres ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Food Samples ◽  
Animal Origin ◽  
Methicillin Resistant

Methicillin-resistant Staphylococcus aureus (MRSA) in foods of animal origin product in Italy

2007 ◽  
Vol 117 (2) ◽  
pp. 219-222 ◽  
Author(s):  
G. Normanno ◽  
M. Corrente ◽  
G. La Salandra ◽  
A. Dambrosio ◽  
N.C. Quaglia ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Animal Origin ◽  
Methicillin Resistant

P903 Methicillin-resistant Staphylococcus aureus strains from animal origin are recognized by IDI-MRSA PCR

2007 ◽  
Vol 29 ◽  
pp. S234
Author(s):  
R. Roosendaal ◽  
J.A.J.W. Kluytmans ◽  
J.H.C. Woudenberg ◽  
X. Huijsdens ◽  
C.M.J.E. Vandenbroucke-Grauls
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Animal Origin ◽  
Methicillin Resistant

scholarly journals Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia

Antibiotics ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 26 ◽  
Author(s):  
Jelena Asanin ◽  
Dusan Misic ◽  
Ksenija Aksentijevic ◽  
Zoran Tambur ◽  
Bojan Rakonjac ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sccmec Type ◽  
Companion Animals ◽  
Antibiotic Resistance Genes ◽  
Variable Number ◽  
Animal Origin ◽  
Methicillin Resistant ◽  
Genetic Profiling ◽  
Biocide Resistance

The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates—30 obtained from humans and six from companion animals—were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL—Panton–Valentine leukocidin, ETs—exfoliative toxins, TSST—toxic shock syndrome toxin, SEs—staphylococcal enterotoxins, and MSCRAMMs—microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6′)-Ia, erm(A), erm(B), erm(C), tet(K), tet(M), fexA, and catpC221. All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission.

Prevalence and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus in Foods of Animal Origin, Turkey

2016 ◽  
Vol 79 (11) ◽  
pp. 1990-1994 ◽  
Author(s):  
BELGİN SİIRİKEN ◽  
TUBA YILDIRIM ◽  
AKİF KORAY GÜNEY ◽  
İRFAN EROL ◽  
BELMA DURUPINAR
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
Raw Milk ◽  
Animal Origin ◽  
Methicillin Resistant ◽  
Selection For

ABSTRACT In the present study, 175 coagulase-positive Staphylococcus (CPS) isolates recovered from samples of beef (n = 110), raw milk n = 56), and fish (n = 9) were analyzed for methicillin resistance using MIC and PCR assays. Methicillin-resistant (MR) Staphylococcus aureus (SA) isolates were then characterized using pulsed-field gel electrophoresis (PFGE). According to findings, 62 (35.4%) of the isolates (44 from beef, 9 from milk, and 9 from fish) were identified as S. aureus based on the presence of the nuc gene. MRCPS was detected in 18 (10.3%) of 175 CPS isolates based on the presence of the mecA gene. Among these isolates, 15 (24.2%) were MRSA: 4 (26.7%) from beef, 2 (13.3%) from milk, and 9 (60%) from fish. However, based on the MIC assay, 21 (12.0%) of the CPS isolates (1 from beef, 15 from milk, and 5 from fish) were MRCPS, indicating a discrepancy between the results of these two methods. The PFGE results indicated genetic heterogeneity of the isolates; six PFGE clusters were found. These results confirm that MRSA is present in foods of animal origin, which is a concern to human health, and indicate the importance of method selection for determination of methicillin resistance. The identity of MR isolates should be verified by PCR to obtain more reliable results.

Comparative analysis of Methicillin-Resistant Staphylococcus aureus (MRSA) and Borderline Oxacillin Resistant Staphylococcus aureus (BORSA) in community and food of animal origin

2020 ◽  
Vol 367 (23) ◽  
Author(s):  
Asima Zehra ◽  
Maliha Gulzar ◽  
Randhir Singh ◽  
Simranpreet Kaur ◽  
J P S Gill
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sccmec Type ◽  
Community Setting ◽  
Animal Origin ◽  
Sample Type ◽  
Community Settings ◽  
Genetic Determinants ◽  
Methicillin Resistant ◽  
Food Of Animal Origin

ABSTRACT The aim of this study was to illustrate the relative pervasiveness of Borderline Oxacillin Resistant Staphylococcus aureus (BORSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) in community and food of animal origin and their relationship with other genetic determinants. Staphylococcus aureus isolates were subjected to E-test using the antibiotics: oxacillin, ceftriaxone, cotrimoxazole, vancomycin, genotypic tests for the genes mecA, vanA, blaZ, pvl gene and SCCmec typing. The prevalence of S. aureus (MRSA) in the food of animal origin and community settings was 21% (1.8% MRSA) and 21.9% (7.4% MRSA), respectively. SCCmec type V was prevalent among the food of animal origin, while SCCmec type IVa among the community isolates. The likelihood of MRSA presence among community isolates was three times more than in isolates from chicken and milk samples. Likewise, the likelihood of detecting pvl positive MRSA (pvl+MRSA) isolates was 4-fold higher in the community setting than in the food of animal origin. The mecA negative BORSA (mecA−BORSA) was a frequently observed phenotype among S. aureus isolates. Also, co-detection of pvl and cotrimoxazol resistance was reported in this study although there was no noteworthy correlation of cotrimoxazol resistance with the type of sample. Isolates from milk and community settings exhibit higher minimum inhibitory concentration to vancomycin (Vancomycin MIC creep, 2–4 µg/mL). Significance Current study provides the information on the statistical relationship between the genetic determinants of S. aureus with respect to sample type, and additionally the correlation that exists between the pvl and MRSA, pvl and cotrimoxazol resistance, vancomycin MIC and MRSA/Methicillin-Susceptible S. aureus (MSSA).

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