Cultivar differences in the influence of a short period of cold storage on ethylene biosynthesis in apples

1997 ◽  
Vol 10 (1) ◽  
pp. 21-27 ◽  
Author(s):  
C. Larrigaudiere ◽  
J. Graell ◽  
J. Salas ◽  
M. Vendrell
HortScience ◽  
2008 ◽  
Vol 43 (3) ◽  
pp. 655-660 ◽  
Author(s):  
Hiroshi Iwanami ◽  
Shigeki Moriya ◽  
Nobuhiro Kotoda ◽  
Sae Takahashi ◽  
Kazuyuki Abe

To compare changes in fruit quality during cold storage with those during shelf life conditions, flesh firmness and titratable acidity (TA) were measured during storage in 20 apple (Malus ×domestica Borkh.) cultivars. Fruit of each cultivar were divided into two groups and stored in chambers controlled at 20 ± 2 °C and 85 ± 5% relative humidity (RH) (shelf life conditions) or 0.5 ± 0.3 °C and 95 ± 5% RH (cold storage). Five of the stored fruit were removed for measurements at 5- or 10-d intervals for 40 d and at 1-month intervals until 10 months after harvest at 20 °C and 0.5 °C, respectively. Data for firmness and TA were subjected to a linear regression and a nonlinear regression, respectively. Moreover, to determine the advantages of 0.5 °C storage over 20 °C storage for retaining firmness and TA, the effect of storage type on extending the storage period was introduced as a parameter. The estimate of the effect of storage type showed that firmness and TA could be retained 8.9 and 3.7 times, respectively, longer at 0.5 °C than 20 °C, independently of the cultivar. Therefore, firmness and TA after cold storage could be predicted by the change in firmness and TA during shelf life conditions. Moreover, cultivar differences regarding quality change under cold storage could be determined in a short period after harvest because the cultivar differences under shelf life conditions were detected within 1 month after harvest.


Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 297
Author(s):  
Ana Belén García ◽  
Eleonora Longo ◽  
Mª Carmen Murillo ◽  
Ruperto Bermejo

Nowadays, there is a growing interest in finding new coloring molecules of natural origin that can increase and diversify the offer of natural food dyes already present in the market. In the present work, a B-phycoerythrin extract from the microalgae Porphyridium cruentum was tested as a food colorant in milk-based products. Using spectroscopy and colorimetry, the extract was characterized and gave evidence of good properties and good stability in the pH range between 4.0 and 9.0. Coloring studies were conducted to demonstrate that samples carrying the pink extract could be used for simulating the pink color of marketed milk-based products. The staining factors, representing the amount of pink protein to be added to reproduce the color of strawberry commercial products, ranged between 1.6 mg/L and 49.5 mg/L, being sufficiently low in all samples. Additionally, color stability during a short period of cold storage was studied: it demonstrated that the three tested types of dairy products remained stable throughout the 11-day analysis period with no significant changes. These results prove the potential of the B-phycoerythrin extract as a natural colorant and alternative ingredient to synthetic coloring molecules.


2013 ◽  
Vol 52 (3) ◽  
pp. 1634-1641 ◽  
Author(s):  
Pedro Cerezal Mezquita ◽  
Blanca E. Barragán Huerta ◽  
Jenifer C. Palma Ramírez ◽  
Claudia P. Ortíz Hinojosa

2008 ◽  
Vol 133 (2) ◽  
pp. 290-299 ◽  
Author(s):  
Ahmad Sattar Khan ◽  
Zora Singh

This research was carried out to extend the postharvest storage of japanese plum (Prunus salicina Lindl. cv. Tegan Blue), which has a short shelf life limiting its export potential. The effects of 1.0 μL·L−1 1-methylcyclopropene (1-MCP) and modified atmosphere packaging (MAP), alone or in combination, on quality of mature japanese plum fruit during storage (0 ± 1 °C and 90% ± 5% relative humidity) were investigated. The activities of enzymes of ethylene biosynthesis [1-aminocyclopropane-1-carboxylic acid synthase (ACS), 1-aminocyclopropane-1-carboxylic acid oxidase (ACO), and 1-aminocyclopropane-1-carboxylic acid (ACC) content] and those of cell wall-associated enzymes [exo-polygalacturonase (exo-PG), endo-polygalacturonase (endo-PG), pectin esterase (PE), and endo-1,4-β-D-glucanase (EGase)] were also measured. 1-MCP-treated fruit stored in normal atmosphere or in MAP had lower ACC content and inhibited ethylene production with reduced ACS and ACO activities compared with fruit stored in MAP and in normal atmosphere. Similarly, 1-MCP-treated fruit, stored either in normal atmosphere or in MAP, were firmer with reduced exo-PG, endo-PG, PE, and EGase activities compared with fruit stored in MAP and in normal atmosphere. During storage as well as during ripening, fruit stored in MAP exhibited a higher rate of respiration compared with other treatments. MAP exacerbated the effect of 1-MCP in reduction of ethylene production and fruit softening. 1-MCP application in combination with MAP after 5 and 7 weeks of storage delayed the fruit ripening by 10 and 8 days in contrast with control fruit, respectively. During storage, and as well as in ripe fruit, weight loss was reduced in fruit stored in MAP either with or without 1-MCP application. Control fruit and 1-MCP-treated fruit, stored in a normal atmosphere or in MAP, had the same values for the following parameters: chromaticity value L*, C*, and hue angle, titratable acidity, and concentrations of soluble solids, ascorbic acid, and total antioxidants. In conclusion, 1-MCP application in combination with MAP can be used effectively to reduce the ethylene biosynthesis and fruit softening during cold storage and to extend the storage life up to 7 weeks followed by 8 d of ripening without any adverse effects on the quality of ripe fruit.


2011 ◽  
Vol 59 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Max G. Villalobos-Acuña ◽  
William V. Biasi ◽  
Sylvia Flores ◽  
Cai-Zhong Jiang ◽  
Michael S. Reid ◽  
...  

2021 ◽  
Vol 22 (21) ◽  
pp. 11308
Author(s):  
Yan Wang ◽  
Li Deng ◽  
Junren Meng ◽  
Liang Niu ◽  
Lei Pan ◽  
...  

Stony hard (SH) peach (Prunus persica L. Batsch) fruit does not release ethylene and has very firm and crisp flesh at ripening, both on- and off-tree. Long-term cold storage can induce ethylene production and a serious risk of chilling injury in SH peach fruit; however, the regulatory mechanism underlying ethylene production in stony hard peach is relatively unclear. In this study, we analyzed the phytohormone levels, fruit firmness, transcriptome, and lipidome changes in SH peach ‘Zhongtao 9’ (CP9) during cold storage (4 °C). The expression level of the ethylene biosynthesis gene PpACS1 and the content of ethylene in SH peach fruit were found to be upregulated during cold storage. A peak in ABA release was observed before the release of ethylene and the genes involved in ABA biosynthesis and degradation, such as zeaxanthin epoxidase (ZEP) and 8’-hydroxylase (CYP707A) genes, were specifically induced in response to low temperatures. Fruit firmness decreased fairly slowly during the first 20 d of refrigeration, followed by a sharp decline. Furthermore, the expression level of genes encoding cell wall metabolic enzymes, such as polygalacturonase, pectin methylesterase, expansin, galactosidase, and β-galactosidase, were upregulated only upon refrigeration, as correlated with the decrease in fruit firmness. Lipids belonging to 23 sub-classes underwent differential rearrangement during cold storage, especially ceramide (Cer), monoglycosylceramide (CerG1), phosphatidic acid (PA), and diacyglyceride (DG), which may eventually lead to ethylene production. Exogenous PC treatment provoked a higher rate of ethylene production. We suspected that the abnormal metabolism of ABA and cell membrane lipids promotes the production of ethylene under low temperature conditions, causing the fruit to soften. In addition, ERF transcription factors also play an important role in regulating lipid, hormone, and cell wall metabolism during long-term cold storage. Overall, the results of this study give us a deeper understanding of the molecular mechanism of ethylene biosynthesis during the postharvest storage of SH peach fruit under low-temperature conditions.


PEDIATRICS ◽  
1972 ◽  
Vol 50 (6) ◽  
pp. 928-930
Author(s):  
N. C. Bethlenfalvay ◽  
Jose M. Louro ◽  
Harry A. Greer

The existence of two distinct embryonic hemoglobins (Hb Gower-1 and Hb Gower-2) in young fetuses up to 85 mm crown-rump length has been well established. Persistence of Hb Gower-2 to (and for a short period after) birth associated with the trisomy D1 syndrome has been well documented. With rare exceptions, however, electrophoretic documentation of this hemoprotein was not accomplished in the majority of subsequently reported cases. Marked instability of Hb Gower-2, especially on cold storage has received but one mention in the literature. The purpose of this paper is to report a case of D/D translocation trisomy in which the demonstration of Hb Gower-2 would not have been possible without this knowledge.


2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Alberto Lázaro ◽  
Blanca Humanes ◽  
Juan Carlos Jado ◽  
Marina Mojena ◽  
María Ángeles González-Nicolás ◽  
...  

Warm ischemia (WI) produces a significant deleterious effect in potential kidney grafts. Hypothermic machine perfusion (HMP) seems to improve immediate graft function after transplant. Our aim was to analyze the effect of short pretransplant periods of pulsatile HMP on histology and renal injury in warm-ischemic kidneys. Twelve minipigs were used. WI was achieved in the right kidney by applying a vascular clamp for 45 min. After nephrectomy, autotransplant was performed following one of two strategies: cold storage of the kidneys or cold storage combined with perfusion in pulsatile HMP. The graft was removed early to study renal morphology, inflammation (fibrosis), and apoptosis. Proinflammatory activity and fibrosis were less pronounced after cold storage of the kidneys with HMP than after cold storage only. The use of HMP also decreased apoptosis compared with cold storage only. The detrimental effects on cells of an initial and prolonged period of WI seem to improve with a preservation protocol that includes a short period of pulsatile HMP after cold storage and immediately before the transplant, in comparison with cold storage only.


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