Characterization of the “Estrogenicity” of tamoxifen and raloxifene in HepG2 cells: Regulation of gene expression from an ERE controlled reporter vector versus regulation of the endogenous SHBG and PS2 genes

Transcriptional regulation in eukaryotes occurs within a chromatin setting, and is strongly influenced by the post-translational modification of histones, the building blocks of chromatin, such as methylation, phosphorylation and acetylation. Acetylation is probably the best understood of these modifications: hyperacetylation leads to an increase in the expression of particular genes, and hypoacetylation has the opposite effect. Many studies have identified several large, multisubunit enzyme complexes that are responsible for the targeted deacetylation of histones. The aim of this review is to give a comprehensive overview of the structure, function and tissue distribution of members of the classical histone deacetylase (HDAC) family, in order to gain insight into the regulation of gene expression through HDAC activity. SAGE (serial analysis of gene expression) data show that HDACs are generally expressed in almost all tissues investigated. Surprisingly, no major differences were observed between the expression pattern in normal and malignant tissues. However, significant variation in HDAC expression was observed within tissue types. HDAC inhibitors have been shown to induce specific changes in gene expression and to influence a variety of other processes, including growth arrest, differentiation, cytotoxicity and induction of apoptosis. This challenging field has generated many fascinating results which will ultimately lead to a better understanding of the mechanism of gene transcription as a whole.

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Phenotypic characterization of rat hepatoma cell lines and lineage-specific regulation of gene expression by differentiation agents

Differentiation ◽

10.1111/j.1432-0436.1998.00215.x ◽

1998 ◽

Vol 63 (4) ◽

pp. 215-223 ◽

Cited By ~ 21

Author(s):

Isabel Zvibel ◽

Shlomo Brill ◽

Anthony S. Fiorino ◽

Lola M. Reid

Keyword(s):

Gene Expression ◽

Cell Lines ◽

Hepatoma Cell ◽

Regulation Of Gene Expression ◽

Phenotypic Characterization ◽

Hepatoma Cell Lines ◽

Specific Regulation ◽

Rat Hepatoma

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Characterization of theDictyostelium discoideumCellulose-binding Protein CelB and Regulation of Gene Expression

Journal of Biological Chemistry ◽

10.1074/jbc.272.42.26166 ◽

1997 ◽

Vol 272 (42) ◽

pp. 26166-26172 ◽

Cited By ~ 5

Author(s):

Ramachandran Ramalingam ◽

Herbert L. Ennis

Keyword(s):

Gene Expression ◽

Binding Protein ◽

Regulation Of Gene Expression

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(296) Genetic and Molecular Characterization of Putative Ethylene Mutants in Antirrhinum majus

HortScience ◽

10.21273/hortsci.41.4.1079b ◽

2006 ◽

Vol 41 (4) ◽

pp. 1079B-1079

Author(s):

Leslie Heffron ◽

Emily Jordan ◽

Jackie Nugent ◽

Schuyler Korban

Keyword(s):

Gene Expression ◽

Culture Medium ◽

Regulation Of Gene Expression ◽

Acc Synthase ◽

Antirrhinum Majus ◽

Cdna Clones ◽

Complementation Groups ◽

Exogenous Ethylene ◽

Mutant Lines

Sixteen putative ethylene mutant Antirrhinum majus (snapdragon) lines, derived from 1-aminocyclopropane-1-carboxylic acid (ACC) screening, were crossed in a full diallel that included the wild-type line to determine allelism/complementation groups. Seeds from these crosses were screened on a tissue culture medium containing 5 μM ACC to elucidate the response to exogenous ethylene treatment. Additionally, five of the mutant lines along with an inbred control, from which the mutants were derived, were analyzed using RT-PCR to determine regulation of gene expression in vegetative (roots, shoots, leaves, and sepals) and floral (six stages of flowering, from green bud to post-pollination) tissues using six different ACC synthase (ACS) cDNA clones and two different ethylene receptor (ETR) cDNA clones, all derived from Antirrhinum majus, as probes. Differential regulation of gene expression for ACS and ETR were observed in some tissues and at different stages of floral development.

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