Metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide] at 1 × 10−5and 1 × 10−4M increased the leakage of previously absorbed,32P-labeled orthophosphate from the roots of onion (Allium cepaL.), a susceptible species, by 14 and 41 times the control values, respectively. A significant amount of32P leaked from the roots of the moderately susceptible species, cotton (Gossypium hirsutumL. ‘DPL 61′) and cucumber (Cucumis sativusL. ‘Ashley′), whereas no significant loss of32P occurred from two tolerant species, soybean [Glycine max(L.) Merr. ‘Bragg′] and corn (Zea maysL. ‘Pioneer 3369A′). At either 1 × 10−7or 1 × 10−6M, 1,8-naphthalic anhydride (NA) prevented32P leakage from onion roots in the presence of 1 × 10−5M metolachlor. High concentrations of NA [0.1% (w/v) suspensions], however, promoted32P leakage and did not protect onion roots from the leakage induced by high concentrations (1 × 10−4M) of metolachlor. Neither metolachlor nor alachlor [2-chloro-2′,6′-diethyl-N-(methoxymethyl)acetanilide], at 1 × 10−4M, inhibited the uptake of acetate-2-14C or malonic acid-2-14C into excised cotton root tips or the incorporation of the precursors into lipids. Similarly, neither herbicide inhibited phospholipid synthesis by cotton root tips. Incorporation of14C-choline chloride into phosphatidylcholine was not significantly inhibited by metolachlor.
Genomic Analysis of the DNA Replication Timing Program during Mitotic S Phase in Maize (Zea mays) Root Tips