A multiplex polymerase chain reaction for a differential diagnosis of Plasmodium falciparum and Plasmodium vivax

Plasmodium falciparum and Plasmodium vivax are co-endemic in Ethiopia. This study investigated whether mixed infections were missed by microscopy from a 2017 therapeutic efficacy study at two health facilities in Ethiopia. All patients (N = 304) were initially classified as having single-species P. falciparum (n = 148 samples) or P. vivax infections (n = 156). Dried blood spots were tested for Plasmodium antigens by bead-based multiplex assay for pan-Plasmodium aldolase, pan-Plasmodium lactate dehydrogenase, P. vivax lactate dehydrogenase, and histidine-rich protein 2. Of 304 blood samples, 13 (4.3%) contained both P. falciparum and P. vivax antigens and were analyzed by polymerase chain reaction for species-specific DNA. Of these 13 samples, five were confirmed by polymerase chain reaction for P. falciparum/P. vivax co-infection. One sample, initially classified as P. vivax by microscopy, was found to only have Plasmodium ovale DNA. Plasmodium falciparum/P. vivax mixed infections can be missed by microscopy even in the context of a therapeutic efficacy study with multiple trained readers.

Download Full-text

Semi-nested, multiplex polymerase chain reaction for detection of human malaria parasites and evidence of Plasmodium vivax infection in Equatorial Guinea.

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.1999.60.183 ◽

1999 ◽

Vol 60 (2) ◽

pp. 183-187 ◽

Cited By ~ 107

Author(s):

J M Rubio ◽

P J Berzosa ◽

M L García ◽

M Micó ◽

M Edú ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Plasmodium Vivax ◽

Multiplex Polymerase Chain Reaction ◽

Vivax Infection ◽

Equatorial Guinea ◽

Chain Reaction ◽

Malaria Parasites ◽

Human Malaria ◽

Polymerase Chain

Download Full-text

Polymerase Chain Reaction Detection of Plasmodium vivax and Plasmodium falciparum DNA from Stored Serum Samples: Implications for Retrospective Diagnosis of Malaria

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.2007.77.444 ◽

2007 ◽

Vol 77 (3) ◽

pp. 444-446 ◽

Cited By ~ 28

Author(s):

Ajay R. Bharti ◽

Kailash P. Patra ◽

Raul Chuquiyauri ◽

Joseph M. Vinetz ◽

Margaret Kosek ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Plasmodium Falciparum ◽

Plasmodium Vivax ◽

Polymerase Chain Reaction Detection ◽

Chain Reaction ◽

Serum Samples ◽

Retrospective Diagnosis ◽

Polymerase Chain

Download Full-text

Unusual Presentation of Vivax Malaria with Anaemia, Thrombocytopenia, Jaundice, Renal Disturbance, and Melena: A Report from Malang, a Nonendemic Area in Indonesia

Case Reports in Infectious Diseases ◽

10.1155/2013/686348 ◽

2013 ◽

Vol 2013 ◽

pp. 1-4 ◽

Cited By ~ 1

Author(s):

Loeki Enggar Fitri ◽

Teguh Wahju Sardjono ◽

Bagus Hermansyah ◽

Didi Candradikusuma ◽

Nicole Berens-Riha

Keyword(s):

Polymerase Chain Reaction ◽

Renal Failure ◽

Plasmodium Falciparum ◽

Plasmodium Vivax ◽

Peripheral Blood ◽

Vivax Malaria ◽

18S Rrna ◽

Chain Reaction ◽

Blood Examination ◽

Polymerase Chain

Most of the complications of malaria such as anaemia, thrombocytopenia, jaundice, and renal failure are commonly found inPlasmodium falciparummalaria, but the incidence of severe and complicated vivax malaria tends to be increasing. We report two cases of severePlasmodium vivaxmalaria from Malang, a nonendemic area in Indonesia. Patients exhibited anaemia, thrombocytopenia, jaundice, renal disturbance, and melena. Microscopic peripheral blood examination and amplification of parasite 18s rRNA by polymerase chain reaction showed the presence ofP. vivaxand absence ofP. falciparum. All patients responded well to antimalarial drugs.

Download Full-text