Stable isotope measurement of fatty acid entrapment in adipose tissue

2001 ◽  
Vol 2 (2) ◽  
pp. 44
Author(s):  
K. Evans ◽  
G.C. Burdge ◽  
M.L. Clark ◽  
S.A. Wootton ◽  
K.N. Frayn
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Stable Isotope ◽  
Isotope Measurement ◽  
Stable Isotope Measurement

scholarly journals Tracing the fate of dietary fatty acids: metabolic studies of postprandial lipaemia in human subjects

2011 ◽  
Vol 70 (3) ◽  
pp. 342-350 ◽  
Author(s):  
Barbara Fielding
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid ◽  
Stable Isotope ◽  
Dietary Fat ◽  
Western Society ◽  
Plasma Nefa ◽  
Stable Isotope Tracers ◽  
Isotope Tracers

Most postprandial studies have investigated the response of a single meal, yet the ingestion of sequential meals is more typical in a Western society. The aim of this review is to explain how natural and stable isotope tracers of fatty acids have been used to investigate the metabolism of dietary fat after single and multiple meals, with a focus on in vivo measurements of adipose tissue metabolism. When stable isotope tracers are combined with arteriovenous difference measurements, very specific measurements of metabolic flux across tissues can be made. We have found that adipose tissue is a net importer of dietary fat for 5 h following a single test meal and for most of the day during a typical three-meal eating pattern. When dietary fat is cleared from plasma, some fatty acids ‘spillover’ into the plasma and contribute up to 50% of postprandial plasma NEFA concentrations. Therefore, plasma NEFA concentrations after a meal reflect the balance between intracellular and extracellular lipolysis in adipose tissue. This balance is altered after the acute ingestion of fructose. The enzyme lipoprotein lipase is a key modulator of fatty acid flux in adipose tissue and its rate of action is severely diminished in obese men. In conclusion, in vivo studies of human metabolism can quantify the way that adipose tissue fatty acid trafficking modulates plasma lipid concentrations. This has implications for the flux of fatty acids to tissues that are susceptible to ectopic fat deposition such as the liver and muscle.

Direct separation of boron from Na- and Ca-rich matrices by sublimation for stable isotope measurement by MC-ICP-MS

Talanta ◽  
2010 ◽  
Vol 82 (4) ◽  
pp. 1378-1384 ◽  
Author(s):  
Bo-Shian Wang ◽  
Chen-Feng You ◽  
Kuo-Fang Huang ◽  
Shein-Fu Wu ◽  
Suresh Kumar Aggarwal ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Icp Ms ◽  
Direct Separation ◽  
Isotope Measurement ◽  
Stable Isotope Measurement

Radiogenic and stable isotope measurement using a MC-ICP-MS

2011 ◽  
Vol 2 (Supplement A) ◽  
pp. A125-A128
Author(s):  
Min Seok Choi ◽  
Jong-Sik Ryu ◽  
Sin-Woo Lee ◽  
Chang-Sik Cheong ◽  
Hyung Seon Shin ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Icp Ms ◽  
Isotope Measurement ◽  
Stable Isotope Measurement

Platelet Aggregation in Finnish Men and Its Relation to Fatty Acids in Platelets, Plasma and Adipose Tissue

1985 ◽  
Vol 54 (03) ◽  
pp. 563-569 ◽  
Author(s):  
M K Salo ◽  
E Vartiainen ◽  
P Puska ◽  
T Nikkari
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Platelet Aggregation ◽  
Acid Composition ◽  
Saturated Fatty Acids ◽  
Free Living ◽  
Ω3 Fatty Acids ◽  
Induced Aggregation

SummaryPlatelet aggregation and its relation to fatty acid composition of platelets, plasma and adipose tissue was determined in 196 randomly selected, free-living, 40-49-year-old men in two regions of Finland (east and southwest) with a nearly twofold difference in the IHD rate.There were no significant east-southwest differences in platelet aggregation induced with ADP, thrombin or epinephrine. ADP-induced platelet secondary aggregation showed significant negative associations with all C20-C22 ω3-fatty acids in platelets (r = -0.26 - -0.40) and with the platelet 20: 5ω3/20: 4ω 6 and ω3/ ω6 ratios, but significant positive correlations with the contents of 18:2 in adipose tissue (r = 0.20) and plasma triglycerides (TG) (r = 0.29). Epinephrine-induced aggregation correlated negatively with 20: 5ω 3 in plasma cholesteryl esters (CE) (r = -0.23) and TG (r = -0.29), and positively with the total percentage of saturated fatty acids in platelets (r = 0.33), but had no significant correlations with any of the ω6-fatty acids. Thrombin-induced aggregation correlated negatively with the ω3/6ω ratio in adipose tissue (r = -0.25) and the 20: 3ω6/20: 4ω 6 ratio in plasma CE (r = -0.27) and free fatty acids (FFA) (r = -0.23), and positively with adipose tissue 18:2 (r = 0.23) and 20:4ω6 (r = 0.22) in plasma phospholipids (PL).The percentages of prostanoid precursors in platelet lipids, i. e. 20: 3ω 6, 20: 4ω 6 and 20 :5ω 3, correlated best with the same fatty acids in plasma CE (r = 0.32 - 0.77) and PL (r = 0.28 - 0.74). Platelet 20: 5ω 3 had highly significant negative correlations with the percentage of 18:2 in adipose tissue and all plasma lipid fractions (r = -0.35 - -0.44).These results suggest that, among a free-living population, relatively small changes in the fatty acid composition of plasma and platelets may be reflected in significant differences in platelet aggregation, and that an increase in linoleate-rich vegetable fat in the diet may not affect platelet function favourably unless it is accompanied by an adequate supply of ω3 fatty acids.

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