607: Lipid nanoparticles for inhaled delivery of mRNA therapeutics

Aim: Chemically modified mRNA offers a novel approach to treat disease. Due to susceptibility to extracellular nucleases in vivo, dosed modified mRNA therapeutics can benefit from encapsulation within novel delivery systems, such as lipid nanoparticles (LNPs). To understand the holistic effect of dosing LNP-encapsulated modified mRNA therapeutics can require bioanalysis of several components including the mRNA, protein and LNP. Methodology: These components can require bespoke preanalytical strategies to preserve analyte integrity to achieve successful analysis. Here we describe the sample collection, processing steps and bioanalytical technologies that can be used to overcome these challenges. Discussion: Understanding the biodistribution and holistic effects of the different components allow the pharmaceutical industry to evaluate safety and efficacy of modified mRNA therapeutics.

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Lipid Nanoparticles for Organ-Specific mRNA Therapeutic Delivery

Pharmaceutics ◽

10.3390/pharmaceutics13101675 ◽

2021 ◽

Vol 13 (10) ◽

pp. 1675

Author(s):

Magdalena M. Żak ◽

Lior Zangi

Keyword(s):

Lipid Nanoparticles ◽

Target Cells ◽

Therapeutic Delivery ◽

The Past ◽

Recent Success ◽

Organ Specific ◽

Mrna Therapeutics ◽

Mrna Technology ◽

Specific Mrna

Advances in the using in vitro transcribed (IVT) modRNA in the past two decades, especially the tremendous recent success of mRNA vaccines against SARS-CoV-2, have brought increased attention to IVT mRNA technology. Despite its well-known use in infectious disease vaccines, IVT modRNA technology is being investigated mainly in cancer immunotherapy and protein replacement therapy, with ongoing clinical trials in both areas. One of the main barriers to progressing mRNA therapeutics to the clinic is determining how to deliver mRNA to target cells and protect it from degradation. Over the years, many different vehicles have been developed to tackle this issue. Desirable vehicles must be safe, stable and preferably organ specific for successful mRNA delivery to clinically relevant cells and tissues. In this review we discuss various mRNA delivery platforms, with particular focus on attempts to create organ-specific vehicles for therapeutic mRNA delivery.

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Enhancement of oral bioavailability of pentoxifylline by solid lipid nanoparticles

Journal of Liposome Research ◽

10.1080/08982100903161456 ◽

2009 ◽

Vol 00 (00) ◽

pp. 090820062440031-9 ◽

Cited By ~ 1

Author(s):

Jaleh Varshosaz ◽

Mohsen Minayian ◽

Elaheh Moazen

Keyword(s):

Solid Lipid Nanoparticles ◽

Oral Bioavailability ◽

Lipid Nanoparticles ◽

Solid Lipid

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Development and optimization of solid lipid nanoparticles of amikacin by central composite design

Journal of Liposome Research ◽

10.1080/08982100903103904 ◽

2009 ◽

Vol 00 (00) ◽

pp. 090721051030036-8

Author(s):

Jaleh Varshosaz ◽

Solmaz Ghaffari ◽

Mohammad Reza Khoshayand ◽

Fatemeh Atyabi ◽

Shirzad Azarmi ◽

...

Keyword(s):

Central Composite Design ◽

Solid Lipid Nanoparticles ◽

Lipid Nanoparticles ◽

Solid Lipid ◽

Central Composite

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Solid lipid nanoparticles for oral delivery of curcumin

Planta Medica ◽

10.1055/s-0033-1352358 ◽

2013 ◽

Vol 79 (13) ◽

Author(s):

C Righeschi ◽

M Bergonzi ◽

B Isacchi ◽

A Bilia

Keyword(s):

Solid Lipid Nanoparticles ◽

Oral Delivery ◽

Lipid Nanoparticles ◽

Solid Lipid

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SOLID LIPID NANOPARTICLES FOR ORAL DELIVERY OF SILIBININ: FORMULATION, CHARACTERIZATION AND EVALUATION USING PAMPA AND CACO-2 CELL MODELS

10.1055/s-0037-1608582 ◽

2017 ◽

Author(s):

V Piazzini ◽

G Graverini ◽

G Vanti ◽

E Bigagli ◽

L Cinci ◽

...

Keyword(s):

Solid Lipid Nanoparticles ◽

Oral Delivery ◽

Lipid Nanoparticles ◽

Cell Models ◽

Solid Lipid

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Development of Solid Lipid Nanoparticles of Lamivudine for Brain Targeting

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v1i1.8837 ◽

2009 ◽

Vol 1 (1) ◽

Cited By ~ 1

Author(s):

Pravin Patil ◽

Anil Sharma ◽

Subhash Dadarwal ◽

Vijay Sharma

Keyword(s):

Drug Delivery ◽

Drug Release ◽

Solid Lipid Nanoparticles ◽

Rotation Speed ◽

Lipid Nanoparticles ◽

Brain Targeting ◽

Brain Penetration ◽

Solid Lipid ◽

Diffusion Technique

The objective of present investigation was to enhance brain penetration of Lamivudine, one of the most widely used drugs for the treatment of AIDS. This was achieved through incorporating the drug into solid lipid nanoparticles (SLN) prepared by using emulsion solvent diffusion technique. The formulations were characterized for surface morphology, size and size distribution, percent drug entrapment and drug release. The optimum rotation speed, resulting into better drug entrapment and percent yield, was in the range of 1000-1250 r/min. In vitro cumulative % drug release from optimized SLN formulation was found 40-50 % in PBS (pH-7.4) and SGF (pH-1.2) respectively for 10 h. After 24 h more than 65 % of the drug was released from all formulations in both mediums meeting the requirement for drug delivery for prolong period of time.

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