Characterization of whole body pain in patients with interstitial cystitis/ bladder painful syndrome: Relationships between pain sites, urinary symptoms and non-urologic associated syndromes

AbstractThe renal proximal tubule is responsible for re-absorption of the majority of the glomerular filtrate and its proper function is necessary for whole-body homeostasis. Aging, certain diseases and chemical-induced toxicity are factors that contribute to proximal tubule injury and chronic kidney disease progression. To better understand these processes, it would be advantageous to generate renal tissues from human induced pluripotent stem cells (iPSC). Here, we report the differentiation and characterization of iPSC lines into proximal tubular-like cells (PTL). The protocol is a step wise exposure of small molecules and growth factors, including the GSK3 inhibitor (CHIR99021), the retinoic acid receptor activator (TTNPB), FGF9 and EGF, to drive iPSC to PTL via cell stages representing characteristics of early stages of renal development. Genome-wide RNA sequencing showed that PTL clustered within a kidney phenotype. PTL expressed proximal tubular-specific markers, including megalin (LRP2), showed a polarized phenotype, and were responsive to parathyroid hormone. PTL could take up albumin and exhibited ABCB1 transport activity. The phenotype was stable for up to 7 days and was maintained after passaging. This protocol will form the basis of an optimized strategy for molecular investigations using iPSC derived PTL.

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MP77-10 URINARY AGRIN CLEAVAGE PRODUCTS PROVIDE PHENOTYPIC CHARACTERIZATION OF INTERSTITIAL CYSTITIS PATIENTS

The Journal of Urology ◽

10.1016/j.juro.2016.02.1924 ◽

2016 ◽

Vol 195 (4S) ◽

Author(s):

Laura Lamb ◽

Peter Levanovich ◽

Andrew Vereecke ◽

Bernadette Zwaans ◽

Sarah Bartolone ◽

...

Keyword(s):

Interstitial Cystitis ◽

Phenotypic Characterization

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Silver Nanoparticles: An Instantaneous Solution for Anticancer Activity against Human Liver (HepG2) and Breast (MCF-7) Cancer Cells

Metals ◽

10.3390/met12010148 ◽

2022 ◽

Vol 12 (1) ◽

pp. 148

Author(s):

Abdulaziz A. Al-Khedhairy ◽

Rizwan Wahab

Keyword(s):

Silver Nanoparticles ◽

Cancer Cells ◽

Human Liver ◽

Quantitative Polymerase Chain Reaction ◽

Cell Types ◽

Whole Body ◽

Low Concentrations ◽

Excellent Control ◽

Mcf 7

Cancer is a cataclysmic disease that affects not only the target organ, but also the whole body. Metal-based nanoparticles (NPs) have recently emerged as a better option for the treatment of this deadly disease. Accordingly, the present work describes a means to control the growth of cancer cells by using colloidal silver nanoparticles (AgNPs) processed via homemade solutions and the characterization of these materials. The AgNPs may become an instantaneous solution for the treatment of these deadly diseases and to minimize or remove these problems. The AgNPs exhibit excellent control of the growth rate of human liver (HepG2) and breast (MCF-7) cancer cells, even at a very low concentrations. The cytotoxic effects of AgNPs on HepG2 and MCF-7 cancer cells were dose dependent (2–200 μg/mL), as evaluated using MTT and NRU assays. The production of reactive oxygen species (ROS) was increased by 136% and 142% in HepG2 and MCF-7 cells treated with AgNPs, respectively. The quantitative polymerase chain reaction (qPCR) data for both cell types (HepG2 and MCF-7) after exposure to AgNPs showed up- and downregulation of the expression of apoptotic (p53, Bax, caspase-3) and anti-apoptotic (BCl2) genes; moreover, their roles were described. This work shows that NPs were successfully prepared and controlled the growth of both types of cancer cells.

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Accurate characterization of 99mTc-MDP uptake in extraosseous neoplasm mimicking bone metastasis on whole-body bone scan: contribution of SPECT/CT

BMC Medical Imaging ◽

10.1186/s12880-019-0345-1 ◽

2019 ◽

Vol 19 (1) ◽

Author(s):

Linqi Zhang ◽

Qiao He ◽

Tao Zhou ◽

Bing Zhang ◽

Wei Li ◽

...

Keyword(s):

Bone Metastasis ◽

Bone Scan ◽

Whole Body ◽

Body Bone

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Time of symptom onset and histologic findings in bladder pain syndrome/interstitial cystitis: new findings on useful correlations

Urogynaecologia ◽

10.4081/uij.2017.201 ◽

2017 ◽

Vol 30 (1) ◽

Author(s):

Daniele Porru ◽

Valentina Bobbi ◽

Carmelo Di Franco ◽

Alessandra Viglio ◽

Mattia Novario ◽

...

Keyword(s):

Interstitial Cystitis ◽

Symptom Onset ◽

Pain Syndrome ◽

Bladder Pain Syndrome ◽

Detrusor Muscle ◽

Comorbid Conditions ◽

Bladder Pain ◽

New Findings ◽

Painful Syndrome ◽

Mast Cell Count

The aim was to find out whether a correlation exists between denudation of urothelium and time of symptom onset in patients with bladder pain syndrome/interstitial cystitis (BPS/IC), and to search a correlation between impact of symptoms evaluated with ICSI-ICPI and the presence of comorbid conditions associated with BPS/IC. Ninety-seven consecutive patients underwent cystoscopy under general anesthesia to classify those cases suspected of being affected from BPS/IC. Three cold bladder biopsies were taken including detrusor muscle. Statistical analysis showed significant correlation between IC/BPS duration and the presence of Hunner’s lesions (P<0.023). Hunner’s lesion with cystoscopy and histological evidence of urothelial denudation with bladder biopsy appear to be items related to IC/BPS duration. Thus an early diagnosis allows to start an appropriate therapeutic approach and prevent a more severe evolution of this multifaceted painful syndrome. Our study shows a correlation between time of symptom onset and evidence of urothelial denudation and with detrusor mast cell count in the whole group of patients. IC/BPS duration seem to correlate with the presence of associated diseases.

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