P165 DOSE-RESPONSE CYTOTOXICITY OF HUMAN COLORECTAL ADENOCARCINOMA HT-29 CELLS EXPOSED TO X-RAYS AFTER PRETREATMENT WITH DOCOSAHEXAENOIC ACID

2008 ◽  
Vol 3 ◽  
pp. 98-99
Author(s):  
L. Thadikkaran ◽  
L. Belabed ◽  
P. Nouet ◽  
A. Allal ◽  
F. Buchegger ◽  
...  
Keyword(s):  
Docosahexaenoic Acid ◽  
Dose Response ◽  
Colorectal Adenocarcinoma ◽  
X Rays ◽  
Ht 29

scholarly journals Docosahexaenoic acid accumulates in cardiolipin and enhances HT-29 cell oxidant production

1998 ◽  
Vol 39 (8) ◽  
pp. 1583-1588 ◽  
Author(s):  
Steven M. Watkins ◽  
Lynne C. Carter ◽  
J. Bruce German
Keyword(s):  
Docosahexaenoic Acid ◽  
Oxidant Production ◽  
Ht 29

scholarly journals Differential Effects of Gold Nanoparticles and Ionizing Radiation on Cell Motility between Primary Human Colonic and Melanocytic Cells and Their Cancerous Counterparts

2021 ◽  
Vol 22 (3) ◽  
pp. 1418
Author(s):  
Elham Shahhoseini ◽  
Masao Nakayama ◽  
Terrence J. Piva ◽  
Moshi Geso
Keyword(s):  
Gold Nanoparticles ◽  
Ionizing Radiation ◽  
Cancer Cells ◽  
Cell Lines ◽  
Colorectal Adenocarcinoma ◽  
X Rays ◽  
Cell Adherence ◽  
Cancerous Cell ◽  
Primary Colon ◽  
Radiation Induced

This study examined the effects of gold nanoparticles (AuNPs) and/or ionizing radiation (IR) on the viability and motility of human primary colon epithelial (CCD841) and colorectal adenocarcinoma (SW48) cells as well as human primary epidermal melanocytes (HEM) and melanoma (MM418-C1) cells. AuNPs up to 4 mM had no effect on the viability of these cell lines. The viability of the cancer cells was ~60% following exposure to 5 Gy. Exposure to 5 Gy X-rays or 1 mM AuNPs showed the migration of the cancer cells ~85% that of untreated controls, while co-treatment with AuNPs and IR decreased migration to ~60%. In the non-cancerous cell lines gap closure was enhanced by ~15% following 1 mM AuNPs or 5 Gy treatment, while for co-treatment it was ~22% greater than that for the untreated controls. AuNPs had no effect on cell re-adhesion, while IR enhanced only the re-adhesion of the cancer cell lines but not their non-cancerous counterparts. The addition of AuNPs did not enhance cell adherence. This different reaction to AuNPs and IR in the cancer and normal cells can be attributed to radiation-induced adhesiveness and metabolic differences between tumour cells and their non-cancerous counterparts.

scholarly journals Aqueous Fraction of Nephelium ramboutan-ake Rind Induces Mitochondrial-Mediated Apoptosis in HT-29 Human Colorectal Adenocarcinoma Cells

Molecules ◽  
2012 ◽  
Vol 17 (6) ◽  
pp. 6633-6657 ◽  
Author(s):  
Chim Kei Chan ◽  
Bey Hing Goh ◽  
Muhamad Noor Alfarizal Kamarudin ◽  
Habsah Abdul Kadir
Keyword(s):  
Colorectal Adenocarcinoma ◽  
Aqueous Fraction ◽  
Adenocarcinoma Cells ◽  
Ht 29

Study on the Toxicity of Calix[4]- and [6]-Arenes and their Sulfated Derivatives in Two- and Three-Dimensional Cell Cultures of Colorectal Adenocarcinoma

2021 ◽  
Vol 37 (1) ◽  
pp. 87-94
Author(s):  
S.V. Nikulin ◽  
B.Ya. Alekseev ◽  
A.N Gorbunov ◽  
I.M. Vatsuro ◽  
V.V. Kovalev ◽  
...  
Keyword(s):  
Cell Culture ◽  
Colorectal Adenocarcinoma ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Russian Science ◽  
Adenocarcinoma Cells ◽  
Therapeutic Molecules ◽  
Derivatives Of ◽  
Ht 29 ◽  
Dimensional Cell

A comparative study of the toxicity of two unsubstituted calixarenes consisting of 4 and 6 phenolic fragments, as well as their p-sulfated derivatives, was carried out on the HT-29 colorectal adenocarcinoma cells cultured in two-dimensional (2D) and three-dimensional (3D) formats. It was shown that both unsubstituted calixarenes decrease the viability of tumor cells; calix[4]arene and calix[6]arene exhibited a cytostatic and a cytotoxic effect, respectively. Sulfated derivatives of calixarenes did not have a pronounced toxic effect on HT-29 cells. However, due to their high hydrophilicity and the ability to form adducts with various therapeutic molecules, they can be used for delivery of anticancer drugs. calixarenes, cytotoxicity, HT-29 cells, 2D cell culture, 3D cell culture The work was financially supported by the Russian Science Foundation (project no. 19-15-00397).

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