Vérification en portée A d’une méthode de PCR triplexe pour la détection de Chlamydia trachomatis, Neisseria gonorrhoeae et Mycoplasma genitalium

2018 ◽  
Vol 2018 (503) ◽  
pp. 22-28 ◽  
Author(s):  
Flora Bochereau ◽  
Hélène Séraphin ◽  
Claire Plassart
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Mycoplasma Genitalium

scholarly journals Prevalence of urogenital, anal, and pharyngeal infections with Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycoplasma genitalium: a cross-sectional study in Reunion island

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
A. Calas ◽  
N. Zemali ◽  
G. Camuset ◽  
J. Jaubert ◽  
R. Manaquin ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Mycoplasma Genitalium ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Transmitted Infection ◽  
Cross Sectional ◽  
Sexually Transmitted ◽  
Urogenital Infection ◽  
High Prevalence

Abstract Background Recommendations for sexually transmitted infection (STI) screening vary significantly across countries. This study evaluated the prevalence of urogenital and extragenital infections with Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Mycoplasma genitalium (MG) in patients visiting a French STI clinic in the Indian Ocean region to determine whether current STI screening practices should be updated. Methods This cross-sectional study examined all patients who visited the STI clinic between 2014 and 2015. Triplex polymerase chain reaction screening for CT, NG, and MG was performed on urine, vaginal, pharyngeal, and anal specimens (FTD Urethritis Basic Kit, Fast Track Diagnostics, Luxembourg). Results Of the 851 patients enrolled in the study, 367 were women (367/851, 43.2%) and 484 were men (484/851, 56.0%). Overall, 826 urogenital specimens (826/851, 97.1%), 606 pharyngeal specimens (606/851, 71.2%), and 127 anal specimens (127/851, 14.9%) were taken from enrolled patients. The prevalence of urogenital CT and MG was high in women ≤25 years (19/186, 10.21%; 5/186, 2.69%) and in men who have sex with women ≤30 years (16/212, 7.54%; 5/212, 2.36%). Among patients with urogenital CT infection, 13.7% (7/51) had urethritis. All patients with urogenital MG infection were asymptomatic. Men who have sex with men had a high prevalence of pharyngeal CT (2/45, 4.44%) and NG (3/44, 6.81%) and a high prevalence of anal CT (2/27, 7.41%), NG (2/27, 7.40%), and MG (1/27, 3.70%). After excluding patients with concomitant urogenital infection, extragenital infections with at least 1 of the 3 pathogens were found in 20 swabs (20/91, 21.9%) taken from 16 patients (16/81, 19.7%), all of them asymptomatic. Conclusions Routine multisite screening for CT, NG, and MG should be performed to mitigate the transmission of STIs in high-risk sexually active populations.

Urethritis

2020 ◽  
pp. 1606-1609
Author(s):  
Patrick Horner
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Partner Notification ◽  
Mycoplasma Genitalium ◽  
Urethral Discharge ◽  
Gonococcal Urethritis

Urethritis is defined as detectable urethral inflammation in the presence of symptoms or an observable urethral discharge. It is conventionally classified into gonococcal urethritis (GU, caused by Neisseria gonorrhoeae) and non-gonococcal urethritis (NGU, caused by Chlamydia trachomatis, Mycoplasma genitalium, and other causes, but with no known pathogen detected in over 30% of cases). Diagnosis is by urethral smear and microbiological investigations. Treatment with appropriate antibiotics should be given only to those with proven urethritis, and the diagnosis and its implications should be discussed with the patient. Partner notification is essential, not only to prevent re-infection but also to prevent onward transmission from partner(s) and the development of complications if left untreated.

scholarly journals Microbiological Characteristics of Chlamydia trachomatis and Neisseria gonorrhoeae Infections in South African Women

2015 ◽  
Vol 54 (1) ◽  
pp. 200-203 ◽  
Author(s):  
Jan Henk Dubbink ◽  
Dewi J. de Waaij ◽  
Myrte Bos ◽  
Lisette van der Eem ◽  
Cécile Bébéar ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Trichomonas Vaginalis ◽  
Mycoplasma Genitalium ◽  
Cross Sectional Study ◽  
African Women ◽  
Transmitted Infection ◽  
Cross Sectional ◽  
Content Type ◽  
South African Women

We analyzed data of 263 women with at least one genital or anorectal sexually transmitted infection from a cross-sectional study conducted in rural South Africa. We provide new insights concerning the concurrence ofChlamydia trachomatis,Neisseria gonorrhoeae,Mycoplasma genitalium, andTrichomonas vaginalisinfections as well as the characteristics of bacterial loads.

scholarly journals Cervical Cytology of Samples with Ureaplasma urealyticum, Ureaplasma parvum, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma hominis, and Neisseria gonorrhoeae Detected by Multiplex PCR

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Fabiana Pirani Carneiro ◽  
Andersen Charles Darós ◽  
Adriana Cysneiro Milhomem Darós ◽  
Tércia Maria Mendes Lousa de Castro ◽  
Marcos de Vasconcelos Carneiro ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Multiplex Pcr ◽  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Diagnostic Methods ◽  
Pap Smears ◽  
Ureaplasma Parvum

Introduction. Despite increasing application of molecular diagnostic methods for the detection of sexually transmitted infections, the cytological findings in pap smears of patients with pathogens that can be identified only by PCR are not yet well described. The aim of this study was to describe the most common cytological features in cervical pap smears of patients with Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum detected by multiplex PCR. Methods. Cervical samples for conventional and liquid-based cytology and for multiplex PCR were collected from women ranging from 23 to 54 years old, who underwent routine screening at a gynecological Unit. Results. Multiplex PCR was positive in 36.2% of the samples: Ureaplasma parvum 14.9%, Chlamydia trachomatis 10.6%, Trichomonas vaginalis 10.6%, Mycoplasma hominis 8.5%, Ureaplasma urealyticum 4.2%, Neisseria gonorrhoeae 2.1%, and Mycoplasma genitalium (0). Multiple pathogens were observed in 12.8% of samples. Microscopic cervicitis (≥10 polymorphonuclear leukocytes/epithelial cell) and normal (predominantly lactobacillary) microbiota were the most frequent findings in the samples in which the pathogens were detected alone or in multiple infections, except for samples with Trichomonas vaginalis in which the coccobacillary microbiota was the most common. In samples with microscopic cervicitis and normal microbiota, those with at least one pathogen identified by multiplex PCR were significantly more frequent than those with no pathogen, 66.6% versus 33.3%. Conclusion. Failure to identify an inflammatory agent in pap smear with intense neutrophil exudate may suggest the presence of Ureaplasma parvum, Ureaplasma urealyticum, Chlamydia trachomatis, or Trichomonas vaginalis. A remark on the intensity of inflammation should be made in the reports of cervical pap smears so that this cytological finding can be correlated with clinical and PCR results.

Microbiota do colo uterino por Gram em pacientes com infeção por papilomavírus humano e outras infecções sexualmente transmissíveis

2021 ◽  
Author(s):  
Marina Mara Sousa de Oliveira ◽  
Hyan Staytskowy Magalhães Martins ◽  
Rafael Pereira de Vasconcelos ◽  
Renata Mirian Nunes Eleutério ◽  
José Eleutério Júnior
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium

Introdução: A microbiota vaginal é um complexo sistema com diversidade de microrganismos. A disbiose parece aumentar o risco de infecções, principalmente as sexualmente transmissíveis, entre as quais por papilomavírus humano, agente associado a lesões cervicais. Objetivo: Avaliar os diferentes tipos de microbiota cervical e as suas características no esfregaço de material residual de citologia em meio líquido, associando com o papilomavírus humano e com Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, Trichomonas vaginalis. Métodos: O estudo analisou 179 casos que tinham material residual de citologia em meio líquido. Alíquota do material foi colocado em lâmina adequada, fixado a seco e corado por método de Gram para leitura em microscópio óptico. Outra alíquota foi utilizada para estudo em reação em cadeia da polimerase - transcriptase reversa e multiplex para pesquisas dos microorganismos associados a infecções sexualmente transmissíveis. O teste exato de Fisher com intervalo de confiança foi utilizado para significância estatística. O projeto foi aprovado em comitê de ética sob número 24071519.9.0000.5049 (UniChristus). Resultados: Os casos foram divididos conforme o escore de Nugent aplicado a esfregaços corados pelo método de Gram. Em microbiota cervical normal (escores de 0 a 3), 100 casos (55,86%); em microbiota intermediária (escore de 4 a 6), 51 casos (28,5%); em sugestivo de disbiose (escore de 7 a 10), 28 casos (15,64%). Nos casos de disbiose, foram observados: Chlamydia trachomatis (1[3,57%]), Mycoplasma hominis (7[25%]), Ureaplasma urealyticum (1[3,57%]), papilomavírus humano 16/45 (1[3,57%]), papilomavírus humano de alto risco (AR) (3[10,71%]) e AR e 16/45 (1[3,57%]). No grupo normal, foi a seguinte distribuição: Ureaplasma urealyticum (1[1%]), papilomavírus humano 16 (2[2%]), papilomavírus humano 18/45 (3[3%]), AR (13[13%]). No grupo intermediário, a distribuição foi: Ureaplasma urealyticum (2[3,9%]), papilomavírus humano AR (5[9,8%]) e papilomavírus humano AR, 16 (1[3,9%]). A única diferença significativa foi de Mycoplasma hominis na disbiose (p<0,0001). Conclusão: O estudo não evidenciou uma associação maior no grupo de disbiose com a maioria das infecções sexualmente transmissíveis, no entanto, com Mycoplasma hominis, foi significativo.

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